Cell therapies with T cells or stem cells hold the potential for treatments for a myriad of pathologies including cancer, cardiovascular, neurodegenerative diseases, among others. The integration of endogenous lymphocytes that can infiltrate tumors and the genetically modified T cells enhance the efficacy and applicability of cell therapy treatments for cancer or other diseases. Moreover, the unique properties of self-renewal and differentiation of stem cells offer novel therapeutic approaches for tissue repair and regeneration. Unlocking the cellular mechanisms of reprogramming and differentiation holds promise for new cellular therapies, regenerative medicine, and improving model systems to assess drug efficacy and toxicity.
Regardless of your cell therapy study path, we provide innovative tools to help you realize greater efficiency with our strong manufacturing capabilities coupled with our supply base. For over 45 years, Enzo has been collaborating with researchers worldwide, offering unique solutions to advance cell therapy research and accelerate the development and manufacturing of next-generation CAR-T and stem cell therapies. With our comprehensive catalog of unique products, we are committed to providing the tools and support you need to drive innovation and advancement in groundbreaking discovery in personalized medicine.
At this final step of cellular therapy development and manufacturing, it is crucial to reduce cell damage following cryopreservation and to assess cellular viability
Cells change their functional and phenotypical type for specific tissue repair and regeneration
T Cells Isolation and Selection
Isolation and selection of T cells to be genetically engineered are essential steps in the development of cell-based immunotherapies. One of the main challenges is the heterogeneity of T cells populations as not all T cells are suitable for cell therapy.
Enzo offers an extensive repertoire of high-specificity antibodies for the identification, analysis, selection, and isolation of specific T cell populations from complex mixtures by flow cytometry. These antibodies are instrumental and can be used for positive selection purposes, for example to enrich T cells population, or as negative selection purposes to remove contaminating and unwanted cells from the target population.
Chimeric antigen receptors (CAR) are engineered T cell receptors to give T cells the ability to target a specifc antigen. T cell activation is a critical step for the expression of CAR T cells, wich can be done in the presence of activated B cells, acting as antigen presenting cells (APCs). One of the main challenges is maintaining an adequate level of B cell stimulation to activate the T cells.
MEGACD40L® Protein (soluble)(human) (recombinant)
MEGACD40L® Protein is an optimized, oligomeric construct which mimics in vivo membrane-assisted CD40L aggregation and stimulation without the need for enhancers.
Enzo’s expertise in the production of challenging protein complexes, such as MEGACD40L® protein, results in a product you can rely on for the duration of your study.
Highly purified protein minimizes unwanted immune activation
Guarantees reliable results through consistent lot-to-lot performance
Optimized formulation for improved stability and enhanced activity
Improved Stability and Enhanced
Activity Compared to CD40L and
CD40L with enhancers.
PBMCs were incubated with serum free test media with serially diluted MEGACD40L® Protein, CD40L (ALX-522-015), or CD40L+ Enhancer (ALX-804-034). After treatment the cells were washed and dual stained with mouse anti-human CD19 (PE conjugate) and mouse anti-human CD86 (APC conjugate).The data is presented as the percent of CD86 positive cells per CD19 positive B cells at each concentration. Samples were analyzed by flow cytometry.
T Cells Expansion
In many cases, the number of target T cells in peripheral blood is relatively low. The key at this phase is to develop efficient methods for stimulating the expansion of these cells ex vivo to obtain a sufficient quantity for therapy. Enzo offer a wide range of solutions to scale-up T-cell expansion with our quality, highly tested and cited cytokines and growth factors.
Cytokines and Growth Factors
Validated for high purity, low endotoxin content, and high enzyme kinetics
Consistent and dependable lot-to-lot performances
Superb performance documented in thousands of peer-reviewed publications
Serial dilutions of Human IL-15 (starting at 1 ng/mL) were added to CTLL-2 cells, a mouse T-cell line, with other needed cytokines and stimuli. After 65 hours, cell viability was measured and the linear portion of the curve can be used to calculate the ED50
T cell exhaustion arise from overstimulation, resulting in dysfunctional cells. Exhausted T cells lose their ability to respond effectively to antigens with significant implications for immunotherapy efficacy. Exhausted T cells often upregulate immune checkpoint receptors, such as PD-1 (programmed cell death protein 1) and Lymphocyte-Activation Gene 3 (LAG-3), and exhibit reduced pro-inflammatory cytokines production such as interferon-gamma, (IFN-γ) and interleukin-2 (IL-2), impacting cytotoxicity, and proliferative capacity.
ELISA Kits and Antibodies
Enzo offers hundreds of ELISA kits in both immunometric and competitive assay formats.
High specificity and sensitivity
Rigorously tested for reproducible results, day-after day and lot-after-lot
Highly cited in thousands of scientific publications
TNF-α (human) ELISA kit can detect as little as 8.4 pg/ml.
Stem Cells Isolation and Reprogramming
Stem cells are widely studied and used in multiple applications in regenerative medicine for their ability to self-renew and differentiate in various cell types. MSCs (Mesenchymal Stem Cells) have the potential to differentiate into a limited range of cell types, while iPSCs (induced Pluripotent Stem Cells) have the ability to differentiate into virtually any cell type. Typically derived from adult somatic cells, iPSCs are created through a process called reprogramming. One of the primary challenges at the reprogramming step is the low efficiency of the process, resulting in an incomplete shift from differentiated cells into iPSC.
Reprogramming Factors for Pluripotent Stem Cells
High biological activity
State-of-the-art production ensure lot-to-lot consistency
Highly cited in thousands of scientific publications
Stem cells can be genetically engineered to express edited target gene and therefore correcting disease-causing mutation. This gene modification process enhances the regenerative or immunomodulatory properties of stem cells therapies. The challenge at this stage is to have a controlled replication of stem cells in vitro, to increase their numbers, while maintaining their undifferentiated and self-renewal capabilities. Small molecules and growth factors play a pivotal role in this process by regulating stem cell proliferation and maintaining their pluripotency or multipotency.
Leupeptin
Leupeptin is a competitive and reversible protease inhibitor serine and cysteine proteinases like calpain, trypsin, and papain. Leupeptin is also known to inhibit activation-induced programmed cell death maintaining viability and functionality of stem cells.
Highly cited and validated in several applications
Active in cell cultures and/or protein homogenase for inhibition of proteolysis
Stem cell self-renewal and differentiation involves complex events which lead to the generation of various progeny cells and tissues. For example, iPSCs are capable of differentiating into virtually any cell type in the body. The efficiency of differentiation is essential to achieve full cell maturation and functionality. Optimizing solutions to enhance the efficiency and scale-up of differentiation is an ongoing challenge. Enzo offer an extensive collection of small molecules that can be used to modulate differentiation, biochemical activity, and cell growth.
ALK5 (TGF-ß receptor 1) plays a crucial role in cell differentiation processes. Alk5 inhibitor is designed to block the phosphorylation and activation of ALK5 therefore modulating cell fate determination and differentiation.
Highly validated under a wide range of conditions (highly cited)
Active in vitro and in vivo for cellular differentiation and the culture of human pluripotent stem cells
GMP version available
Chemical structure of ALK5 Inhibitor II (CAS 446859-33-2)
This is the last stage of T cells and stem cells therapy development, and it is criticall and challenging the preservation of cellular viability, functionality, and genetic stability during longterm storage. It is also essential to identify any delayed or cumulative effects of cryopreservation-related toxicity.
Enzo as a long and successful track record in identifying, synthesizing, and commercializing valuable known small molecules to efficiently accelerate the development and scale-up the production of cell-based therapies.
Y-27632 is a highly potent, cell permeable, selective and ATP competitive inhibitor of ROCK1 and ROCK2 (IC50=800nM). This small molecule blocks apoptosis of isolated cells, increases their cloning efficiency, and improves the survival rate of T cells and stem cells undergoing cryopreservation.
Selective inhibitor of ROCK1 and ROCK2
Used in the cryopreservation media of stem cells
Highly cited and validate in several applications in cancer and stem cell studies
Brighter and More Stable Dye for Cell Cycle Analysis Compared to Leading Competitor's
HeLa cells were stained at three different concentrations with NUCLEAR-ID Red Cell Cycle Kit (A, C, D) and with leading competitor's red dye kit (B, D, F), for 15 minutes at 37°C. A-B Cells were stained with 2.5 μM dye; C-D cells were stained with 5.0 μM dye; E-F cells were stained with 10.0 μM dye. Results show that NUCLEAR-ID® Red DNA stain requires lower concentration than competitor's dye to visualize dsDNA.
This is a multiplex assay that distinguises between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP andother fluorescent probes (blue or cyan).
Assess effects of cell isolation and preservation techniques, drug treatment effects, reprogramming modulators
Optimized for both fluorescence microscopy and flow cytometry applications
Multiplex Healthy, Early Apoptotic, Late Apoptotic and Necrotic Cells
GFP-CERTIFIED Apoptosis/Necrosis Detection Kit (ENZ-51002) detects four distinct cell states. Mitochondrial GFP-expressing HeLa cells were treated with 2μM Staurosporine for 4 hours. The Apoptosis Detection Reagent (Gold) and Necrosis Detection Reagent (Red) specifically detect cell states with clear spectral separation from mitochondria-associated GFP signal. Healthy cells (A), cells undergoing apoptosis (B), cells undergoing late-stage apoptosis (C), and necrotic cells (D).
Enabling Cell & Gene Therapy Research
Leverage over 45 years of innovation and technical expertise supporting drug development, discovery, research, and diagnostics through our core technology platforms. With over 150,000 citations, GMP and ISO certifications, our highly-sensitive, quality products consistently deliver trusted results. We maintain product integrity and reliability with our in-house U.S. manufacturing facility. We deploy our bench of expert PhD scientists to provide technical, tailored, support for our customers and their projects.
Rely on our extensive experience in innovation, technology development, and manufacturing to support your cell and gene therapy research and development. Our comprehensive Life Sciences Contract Services support the development of customizable, unique, and efficient solutions for all your research needs.