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PDGF-BB (human), (recombinant)

ENZ-PRT257-0100 100 µg 1,115.00 USD
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Product Details

Alternative Name:Platelet-derived growth factor-BB, GDGF, ODGF
Source:Produced in E. coli. Contains 110/220 amino acids.
UniProt ID:P01127
Formulation:Lyophilized from a sterile (0.2 micron) filtered solution containing 10 mM sodium phosphate, pH 7.5.
Purity:≥95% (SDS-PAGE)
Endotoxin Content:≤1 EU/µg protein (LAL test)
Reconstitution:Reconstitute in sterile water at 0.1 mg/mL.
Shipping:Ambient Temperature
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. For long term storage, it is recommended to dilute to working aliquots in a 0.1% BSA solution.
Scientific Background:Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-a, TGF-b and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b.
Regulatory Status:RUO - Research Use Only
PDGF-BB (human), (recombinant) image
Figure 1: Serial dilutions of Human PDGF-BB, starting at 500 ng/mL, were added to NR-6-R 3T3 cells. Cell proliferation was measured and the linear portion of the curve can be used to calculate the ED50.
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PDGF-BB (human), (recombinant) image

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