A Data-Driven Approach

The drug development industry has undertaken significant efforts to identify toxic events at the earliest opportunity during the development process, moving from a predominantly observational science at the level of disease-specific models to a more predictive model focused on target-specific, mechanism-based biological observations. The growth in such Early Safety Assessment initiatives has driven the need for more reliable, cost-effective, high throughput in vitro toxicity assays capable of predicting toxic liabilities prior to investment in more costly pre-clinical and clinical trials.

Cell Based Assays & Biomarker Detection: Comparison of lead compound results with those from compounds of known toxicity allows early elimination of toxic candidates.

CELLESTIAL® Assay Kits, Cell Based Assays for Predictive Toxicology

Our panel of fluorescence-based live cell assays are designed to help assess the impact of toxic agents on overall cell function. Our assays and probes have been developed to produce dyes that are sensitive, specific, and convenient, offering increased photostability, eliminating non-specific dye-associated artifacts to reduce false positives, and compatibility with common dyes and fluorescent markers for multiplex analysis. Our dyes are optimized for the most demanding imaging applications, including microplate-based high content screening, flow cytometry, confocal microscopy, and wide-field fluorescence microscopy, where consistency and reproducibility are essential.

SCREEN-WELL® Toxicity Libraries

SCREEN-WELL® libraries from Enzo Life Sciences for nephrotoxicity, cardiotoxicity, hepatotoxicity, and hematopoietic toxicity contain compounds with defined and diverse organ-associated toxicity profiles. A variety of structurally and mechanistically different compound classes are included, as well as non-toxic controls. Compounds are dissolved in DMSO at 10mM and aliquoted into deepwell plates at 100 or 500μl per well. The libraries are useful for predictive toxicology screening, including high-content protocols.

• Better understand mechanisms of toxicity with your small molecule or candidate-drug of interest.
• Compare your compound with known references for investigative toxicity
• Optimize formulation efficacy and safety

Assessment of Kidney Injury and Nephrotoxicity

Enzo provides a variety of tools to detect markers of acute kidney injury, renal failure, and renal metabolism. These thoroughly validated, high-sensitivity kits are validated for a variety of species and samples types, and are supported by antibodies and proteins to support biomarker detection and characterization.

• KIM-1 ELISA Kit
• NGAL (lipocalin-2) ELISA Kits
• Cystatin C ELISA Kits
• Creatinine Detection Kit
• SCREEN-WELL® Nephrotoxicity library