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SOD activity kit

ELS offers a large collection of superoxide dismutase products for oxidative stress research.
ADI-900-157 5x96 wells 757.00 USD
Do you need bulk/larger quantities?
  • Versatile - differentiate between activity of three SOD isoenzymes: MnSOD, FeSOD, and Cu/ZnSOD
  • Higher throughput - colorimetric 96 well plate format
  • Fast - results in just 10 minutes
This is a versatile assay for the determination of SOD activity. Differentiate between the activity of three SOD isoenzymes: MnSOD, FeSOD, and Cu/ZnSOD. Obtain higher throughput sample analysis with a convenient colorimetric 96 well plate format and fast results in just 10 minutes.

Product Details

Alternative Name:Superoxide dismutase
Applications:Colorimetric detection
Activity assay
Application Notes:For the measurement of total SOD activity in cell lysates and tissue from any species. Cited sample type includes serum and gastric mucosa homogenates (ref.).
Use/Stability:Store all components at 4°C, except SOD standard and Xanthine Oxidase at -20°C.
Shipping:Blue Ice
Contents:Microtiter plate, Standard, SOD buffer concentrate, Xanthine oxidase, Xanthine solution, Triton x-100, WST-1 reagent
Scientific Background:SOD (Superoxide dismutase) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells, they are: EC SOD (extracellular SOD), Cu/Zn SOD (copper and zinc-containing SOD) and Mn SOD (manganese-containing SOD).
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Evaluation of the Antioxidant Activity of Levetiracetam in a Temporal Lobe Epilepsy Model: I.I. Mejía, et al.; Biomedicines 11, 848 (2023), Abstract;
Mitochondria-targeted anti-oxidant AntiOxCIN4 improved liver steatosis in Western diet-fed mice by preventing lipid accumulation due to upregulation of fatty acid oxidation, quality control mechanism and antioxidant defense systems: R. Amorim, et al.; Redox Biol. 55, 102400 (2022), Abstract;
A mitochondria-targeted caffeic acid derivative reverts cellular and mitochondrial defects in human skin fibroblasts from male sporadic Parkinson's disease patients: C.M. Deus, et al.; Redox Biol. 45, 102037 (2021), Abstract; Full Text
Increased oxidative stress contributes to enhance brain amyloidogenesis and blunts energy metabolism in sucrose-fed rat: effect of AMPK activation: L. Camacho-Castillo, et al.; Sci. Rep. 11, 19547 (2021), Abstract;
Cardiovascular Protection Variables Based on Exercise Intensity in Stable Coronary Heart Disease Patients After Coronary Stenting: A Comparative Study: D. Sarvasti, et al.; Vasc. Health Risk Manag. 16, 257 (2020), Abstract; Full Text
Bacillus subtilis CBR05 induces Vitamin B6 biosynthesis in tomato through the de novo pathway in contributing disease resistance against Xanthomonas campestris pv. Vesicatoria: M. Chandrasekaran, et al.; Sci. Rep. 9, 6495 (2019), Abstract; Full Text
Hypoxia-induced PGC-1α Regulates Mitochondrial Function and Tumorigenesis of Colorectal Cancer Cells: C.W. Yun, et al.; Anticancer Res. 39, 4865 (2019), Abstract;
Oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress: A. Stier, et al.; Sci. Rep. 9, 8545 (2019), Application(s): Penguin plasma and red blood cell lysate, Abstract; Full Text
Acylated ghrelin protects aorta damage post-MI via activation of eNOS and inhibition of angiotensin-converting enzyme induced activation of NAD(P)H-dependent oxidase: R.A. Eid, et al.; Ultrastruct. Pathol. 42, 416 (2018), Abstract;
Anti-Diabetic Effect of Cotreatment with Quercetin and Resveratrol in Streptozotocin-Induced Diabetic Rats: D.K. Yang & H.S. Kang; Biomol. Ther. (Seoul) 26, 130 (2018), Application(s): Rat serum, Abstract; Full Text
Benfotiamine treatment activates the Nrf2/ARE pathway and is neuroprotective in a transgenic mouse model of tauopathy: V. Tapias, et al.; Hum. Mol. Genet. 27, 2874 (2018), Abstract;
Rutin hydrate ameliorates cadmium chloride-induced spatial memory loss and neural apoptosis in rats by enhancing levels of acetylcholine, inhibiting JNK and ERK1/2 activation and activating mTOR signalling: G.A. Abdel-Aleem, et al.; Arch. Physiol. Biochem. 124, 367 (2018), Abstract;
Vitamin B6 biosynthetic genes expression and antioxidant enzyme properties in tomato against, Erwinia carotovora subsp. carotovora: M. Chandrasekaran, et al.; Int. J. Biol. Macromol. 116, 31 (2018), Abstract;
Nigella sativa seed extract attenuates the fatigue induced by exhaustive swimming in rats: M. Rahman, et al.; Biomed. Rep. 6, 468 (2017), Abstract; Full Text
Role of NADPH Oxidase as a Mediator of Oxidative Damage in Low-Dose Irradiated and Hindlimb-Unloaded Mice: X.W. Mao, et al.; Radiat. Res. 188, 392 (2017), Application(s): mouse brain homogenates, Abstract;
Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?: S. Emamgholipour, et al.; Biochem. Res. Int. 2016, Article ID 5857940 (2016), Application(s): Measurement of MnSOD Activity in PBMCs, Abstract; Full Text
Cellular Redox Systems Impact the Aggregation of Cu,Zn Superoxide Dismutase Linked to Familial Amyotrophic Lateral Sclerosis: C. Álvarez-Zaldiernas, et al.; J. Biol. Chem. 291, 17197 (2016), Application(s): Activity Assay of wt andG93A mutant SOD1, Abstract; Full Text
Effect of complete hilar versus only renal artery clamping on renal histomorphology following ischemia/reperfusion injury in an experimental model: M. Umul, et al.; Eur. Rev. Med. Pharmacol. Sci. 20, 589 (2016), Application(s): Measurement of SOD activity, Abstract; Full Text
Effects of Resveratrol on Crosstalk between Canonical Β-Catenin/Wnt and FOXO Pathways in Coronary Artery Disease Patients with Metabolic Syndrome: A Case Control Study: M. Shanaki, et al.; Iran. J. Pharm. Res. 15, 547 (2016), Abstract; Full Text
Evidence for possible role of melatonin in reducing oxidative stress in multiple sclerosis through its effect on SIRT1 and antioxidant enzymes: S. Emamgholipour, et al. ; Life Sci. 145, 34 (2016), Application(s): MnSOD activity in PBMCs, Abstract;
Protection of Chinese olive fruit extract and its fractions against advanced glycation endproduct-induced oxidative stress and pro-inflammatory factors in cultured vascular endothelial and human monocytic cells: C.T. Kuo, et al.; J. Funct. Foods 27, 526 (2016), Application(s): EA.hy926 cells ,
Simulated Microgravity and Low-Dose/Low-Dose-Rate Radiation Induces Oxidative Damage in the Mouse Brain: X.W. Mao, et al.; Radiat. Res. 185, 647 (2016), Application(s): mouse brain homogenates, Abstract;
Zinc Is Indispensable in Exercise-Induced Cardioprotection against Intermittent Hypoxia-Induced Left Ventricular Function Impairment in Rats: T.I. Chen, et al.; PLoS One 11, e0168600 (2016), Abstract;
Antidiabetic Effects of Yam (Dioscorea batatas) and Its Active Constituent, Allantoin, in a Rat Model of Streptozotocin-Induced Diabetes: H.-K. Go, et al.; Nutrients 7, 8532 (2015), Application(s): Rat serum, Abstract; Full Text
Antioxidative Activity of Onion Peel Extract in Obese Women: A Randomized, Double-blind, Placebo Controlled Study: K. Kim, et al.; J. Cancer Prev. 20, 202 (2015), Application(s): Determination of superoxide dismutase activity, Abstract; Full Text
Expression and enzyme activity of MnSOD and catalase in peripheral blood mononuclear cells isolated from multiple sclerosis patients: S. Emamgholipour, et al.; Archives of Medical Laboratory Sciences 1, 23 (2015), Application(s): Assay using human cell lysate, Full Text
Mitochondrial Modulation by Epigallocatechin 3-Gallate Ameliorates Cisplatin Induced Renal Injury through Decreasing Oxidative/Nitrative Stress, Inflammation and NF-kB in Mice: H. Pan, et al.; PLoS One 10, e0124775 (2015), Application(s): Assay using mouse mitochondrial fraction , Abstract; Full Text
Altered Thiol Chemistry in Human Amyotrophic Lateral Sclerosis-linked Mutants of Superoxide Dismutase 1: C. Solsona, et al.; J. Biol. Chem. 289, 26722 (2014), Application(s): SOD activity of several SOD mutants, Abstract; Full Text
The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death: Y.T. Tseng, et al.; Phytomedicine 724, 21 (2014), Application(s): SOD activity measured in rat primary mesencephalic neuron lysates, Abstract;
Atorvastatin protects against deleterious cardiovascular consequences induced by chronic intermittent hypoxia: P. Totoson, et al.; Exp. Biol. Med. 238, 223 (2013), Abstract;
Cystine dimethylester loading promotes oxidative stress and a reduction in ATP independent of lysosomal cystine accumulation in a human proximal tubular epithelial cell line: R. Sumayao, et al.; Exp. Physiol. 98, 1505 (2013), Abstract;
Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow: X. Sun, et al.; Antioxid. Redox Signal. 18, 1739 (2013), Abstract;
Effects of curcumin on proinflammatory cytokines and tissue injury in the early and late phases of experimental acute pancreatitis: A. Gulcubuk, et al.; Pancreatology 13, 347 (2013), Application(s): SOD activity measured in rat serum, Abstract;
Ellagitannins from Rubus berries for the control of gastric inflammation: in vitro and in vivo studies: E. Sangiovanni, et al.; PLoS One 8, e71762 (2013), Application(s): SOD activity measured in rat gastric mucosa homogenates, Abstract; Full Text
Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress: J.O. Baek, et al.; Arch. Dermatol. Res. 304, 699 (2012), Abstract;
Human atherosclerotic plaque lipid extract impairs the antioxidant defense capacity of monocytes: A. Szuchman-Sapir, et al.; BBRC 423, 884 (2012), Application(s): SOD activity measured in human cell (THP-1) lysates, Abstract;
Immature Rubus coreanus Shows a Free Radical-Scavenging Effect and Inhibits Cholesterol Synthesis and Secretion in Liver Cells: B. Bhandary, et al.; Indian J. Pharm. Sci. 74, 211 (2012), Abstract; Full Text
Role of carnitine acetyl transferase in regulation of nitric oxide signaling in pulmonary arterial endothelial cells: S. Sharma, et al.; Int. J. Mol. Sci. 14, 255 (2012), Application(s): SOD activity measured in sheep cell (PAEC) lysates, Abstract; Full Text
Vitamin C, resveratrol and lipoic acid actions on isolated rat liver mitochondria: all antioxidants but different: M.P. Valdecantos, et al.; Redox Rep. 15, 207 (2010), Application(s): SOD activity measured in human erythrocytes, Abstract; Full Text

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