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LDH Cytotoxicity WST Assay

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity
 
ENZ-KIT157-0500 500 tests 221.00 USD
 
ENZ-KIT157-2500 5x500 tests 830.00 USD
Do you need bulk/larger quantities?
 
  • Homogenous assay using living cells or non-homogenous assay protocol using cell culture supernatant
  • Stable working solution
The LDH cytotoxicity WST assay is a colorimetric assay kit used to determine cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells.
LDH Cytotoxicity WST Assay mechanism
Mechanism for LDH Cytotoxicity WST Assay
LDH Cytotoxicity WST Assay Cytotoxicity
Difference between the Cell Counting Kit-8 (Prod. No. ALX-850-039) and the LDH Cytotoxicity WST Assay. The Cell Counting Kit-8 determines the number of viable cells in cell proliferation and cytotoxicity assays. On the other hand, the LDH Cytotoxicity WST Assay evaluates the cytotoxic level by measuring the amount of LDH released from damaged cells. Assessing the level of cytotoxicity by combining both the Cell Counting Kit-8 Kit and the LDH Cytotoxicity WST Assay can provide researchers with a complete evaluation of cell health.
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LDH Cytotoxicity WST Assay mechanism LDH Cytotoxicity WST Assay Cytotoxicity

Product Specification

Applications:Colorimetric detection
 
Quantity:500 tests/5ml
2500 tests/5 x 5ml
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:LDH is a stable cytoplasmic enzyme presented in all types of cells and released into the cell culture medium through damaged plasma membrane.
 
Technical Info/Product Notes:Principle: LDH catalyzes dehydrogenation of lactate to pyruvate, reducing NAD to NADH. NADH reduces a water soluble tetrazolium salt (WST) in the presence of an electron mediator to produce an orange formazan dye. The amount of formazan dye produced is proportional to the amount of LDH released into the medium, indicating cytotoxicity.
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Ethanol extract of Allium fistulosum inhibits development of non-alcoholic fatty liver disease: J.T. Hwang, et al.; Nutr. Res. Pract. 12, 110 (2018), Abstract; Full Text

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