CYTAG^® TotalCGH Labeling kit

Complete kit to digest and label DNA for CGH+SNP arrays



ENZ-42674-0010	2x10 Reactions	1,166.00 USD

ENZ-42674-0100	2x100 Reactions	7,736.00 USD
Do you need bulk/larger quantities?

Also available in custom high throughput formats.

Contains CYTAG^® CGH Labeling kit, restriction enzymes AluI and RsaI, and PCR & Gel Clean-up Columns
Compatible with CGH and CGH+SNP arrays
Ability to perform total genomic DNA analysis without amplification or complexity reduction
Provides excellent dynamic analytical range for challenging and complex, heterogeneous samples
QC benchmarked and validated using high resolution arrays
Superior DLR scores (0.09-0.12), exceeding industry standards

Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

With optimized, proprietary reagents, Enzo's CYTAG^® CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification.

Bulk quantity kit, 2 x 1000 reactions, is available upon special request.

CYTAG® TotalCGH Labeling kit Kit box image

SNP analysis of a segment of chromosome 2. DNA was labeled with the Enzo kit (left) or a competitor’s kit (right). DNA was hybridized to Agilent’s CGH+SNP microarray. The Enzo kit cleanly identifies the sequence at SNPs while quantification of the SNP analysis of DNA labeled with a competitor’s labeling system is not as clean.

CYTAG® TotalCGH Labeling kit ComparativeAnalysis

Comparative analysis of chromosome 15. Upon scanning, the quality of the labeling in DNA samples was visually inspected to demonstrate detection of known deletion in the Prader-Willi DNA. This demonstrates the superior labeling obtained with the CGH labeling kit from Enzo Life Sciences when compared with a competitor’s kit.

Please mouse over

Product Details

Applications:	CGH

Use/Stability:	Store PCR & Gel Clean-up Columns at room temperature. All other components should be stored at -20°C. Stable for at least one year after receipt when stored as recommended.

Handling:	Protect Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.

Shipping:	Dry Ice and Ambient Temperature

Long Term Storage:	-20°C

Contents:	-0010 size: ENZ-42671-K010: Primers/Reaction Buffer, 1 x 400 µl; Cyanine 3-dUTP Nucleotide Mix, 2 x 50 µl; Cyanine 5-dUTP Nucleotide Mix, 2 x 50 µl; Klenow Exo-DNA Polymerase, 1 x 20 µl; Stop Buffer, 1 x 100 µl; Nuclease-free Water, 1 x 1 ml ENZ-GEN100-0020: PCR & Gel Clean-up columns, 20 tests ENZ-GEN108-1000: AluI, 1000 units, 10X Cutting Buffer ENZ-GEN109-1000: RsaI, 1000 units, 10X Cutting Buffer -0100 size: ENZ-42671-K100: Primers/Reaction Buffer, 1 x 4 ml; Cyanine 3-dUTP Nucleotide Mix, 1 x 1 ml; Cyanine 5-dUTP Nucleotide Mix, 1 x 1 ml; Klenow Exo-DNA Polymerase, 1 x 0.2 ml; Stop Buffer, 1 x 1 ml; Nuclease-free Water, 1 x 10 ml ENZ-GEN100-0200: PCR & Gel Clean-up columns, 200 tests ENZ-GEN108-1000: AluI, 1000 units, 10X Cutting Buffer ENZ-GEN109-1000: RsaI, 1000 units, 10X Cutting Buffer

Scientific Background:

Technical Info/Product Notes:	Application Note: Using CYTAG^® TotalCGH Labeling Kit for CGH + SNP Array

Regulatory Status:	RUO - Research Use Only

Related Products

	CYTAG^® CGH Labeling kit
	Superior labeling efficiency and better dye incorporation results in less failed runs
	CGH \| Print as PDF

	ENZ-42671-K010	2x10 Reactions	1,012.00 USD

	ENZ-42671-K100	2x100 Reactions	7,423.00 USD
Do you need bulk/larger quantities?

	PCR & Gel Clean-up Kit
	Quick, easy kit for purifying DNA from PCR, gels and labeling reactions for a variety of downstream applications.
	Print as PDF

	ENZ-GEN100-0020	20 tests	60.00 USD

	ENZ-GEN100-0050	50 tests	122.00 USD

	ENZ-GEN100-0200	200 tests	458.00 USD
Do you need bulk/larger quantities?

	AluI
	Restriction enzyme for molecular biology applications
	Produced in an E. coli strain that carries the cloned AluI gene from Arthrobacter luteus., Southern Blot, Cloning, Genotyping, CGH \| Print as PDF

	ENZ-GEN108-1000	1000 U	109.00 USD
Do you need bulk/larger quantities?

	RsaI
	Restriction enzyme for molecular biology applications
	Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides., Southern Blot, Cloning, Genotyping, CGH \| Print as PDF

	ENZ-GEN109-1000	1000 U	76.00 USD
Do you need bulk/larger quantities?

	Human Cot DNA
	Blocks repetitive sequences to prevent nonspecific binding.
	ISH (in situ hybridization), Microarray, Southern Blot, FISH, CGH \| Print as PDF

	ENZ-GEN116-0500	500 µg	288.00 USD
Do you need bulk/larger quantities?

	Human Genomic DNA, Male
	A normal pool of male genomic DNA that can serve as a standard in molecular biology applications
	PCR, Southern Blot, CGH \| Print as PDF

	ENZ-GEN117-0100	100 µg	206.00 USD
Do you need bulk/larger quantities?

	Human Genomic DNA, Female
	A normal pool of female genomic DNA that can serve as a standard in molecular biology applications
	PCR, Southern Blot, CGH \| Print as PDF

	ENZ-GEN118-0100	100 µg	206.00 USD
Do you need bulk/larger quantities?