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DHEA ELISA kit

Highly sensitive ELISA, measuring DHEA in culture supernatants, plasma, serum, saliva, and urine from any species.
 
ADI-900-093 96 wells 408.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-093 (5x96 wells)
  • Specific with low reactivity to structurally related molecules
  • Highly reproducible with less than 9% inter- and intra-assay variation
  • Highly sensitive, detecting low levels of DHEA
  • Fast results for up to 37 samples in duplicate in 5 hours
The DHEA EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. This kit has low reactivity to structurally related molecules and offers sensitive measurement of DHEA in a variety of sample types.
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Product Details

Alternative Name:Dehydroepiandrosterone
 
Sensitivity:2.9 pg/ml (range 12.21 - 50,000 pg/ml)
 
Assay Time:5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of DHEA in culture supernatants, plasma, serum, saliva, and urine from any species.
 
Wavelength:405 nm
 
Species reactivity:Species independent
 
Crossreactivity:DHEA (100%), DHEA sulfate (30.0%), Androstenedione (0.73%), Androsterone (0.29%), Pregnenolone (0.28%), Testosterone (0.10%), Progesterone (0.06%), Estradiol (0.04%) and <0.02%: 20-hydroxycholesterol, Androsterone sulphate, DHEA sodium sulphate, 17-hydroxyepipregnanolone, 19-hydroxyandrostendione, 17-hydroxyprogesterone, Cortisol
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Contents:GxR IgG Microtiter plate, Assay buffer, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Dehydroepiandrosterone (DHEA) is a 17-ketosteroid produced by side chain cleavage of 17-hydroxypregnenolone. DHEA is reversibly converted to its sulfate ester (DHEA-S) and Androstendiol. The human adrenal glands secrete both DHEA and DHEA-S which in turn serve as the precursors for both estrogenic and androgenic steroids. DHEA has been linked to a number of physiological conditions including depression, systemic lupus erythematosus, and rheumatoid arthritis.
 
Technical Info/Product Notes:Cited samples:
Wildlife Endocrinology: Cited Sample Types
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Divergent neural and endocrine responses in wild-caught and laboratory-bred Rattus norvegicus: J. Jacob, et al.; Behav. Brain Res. 432, 113978 (2022), Abstract;
Novel regulation mechanism of adrenal cortisol and DHEA biosynthesis via the endogen ERAD inhibitor small VCP-interacting protein: R. Iihan, et al.; Sci. Rep. 12, 869 (2022), Abstract;
Dehydroepiandrosterone (DHEA) Serum Levels Indicate Cerebrospinal Fluid Levels of DHEA and Estradiol (E2) in Women at Term Pregnancy: P. Habib, et al.; Reprod Sci. 28, 2823 (2021), Abstract;
Exercise Training Promotes Sex-Specific Adaptations in Mouse Inguinal White Adipose Tissue: P. Nigro, et al.; Diabetes 70, 1250 (2021), Abstract;
Neurobiological effects of a probiotic-supplemented diet in chronically stressed male Long-Evans rats: Evidence of enhanced resilience: N.R. Natale, et al.; IBRO Neurosci. Rep. 11, 207 (2021), Abstract;
Resistance exercise-induced increase in muscle 5α-dihydrotestosterone contributes to the activation of muscle Akt/mTOR/p70S6K- and Akt/AS160/GLUT4-signaling pathways in type 2 diabetic rats: N. Horii, et al.; FASEB J. 34, 11047 (2020), Abstract; Full Text
Expression of steroidogenic enzymes in human placenta according to the gestational age: S.H. Hong, et al.; Mol. Med. Rep. 19, 3903 (2019), Abstract;
Maternal-induced shifts in allostatic demands: Reproductive experience alters emotional and cognitive biobehavioral responses in rats (Rattus norvegicus): E.D.K. Sullivan, et al.; Neurosci. Lett. 701, 1 (2019), Application(s): used w. rat fecal samples, Abstract;
Oxidative stress increases the 17, 20-lyase-catalyzing activity of adrenal P450c17 through p38α in the development of hyperandrogenism: W. Zhu, et al.; Mol. Cell. Endocrinol. 484, 25 (2019), Application(s): ELISA using culture supernatants, Abstract;
Gender differences in the relationships among neurosteroid serum levels, cognitive function, and quality of life: C.Y. Chen, et al.; Neuropsychiatr. Dis. Treat. 14, 2389 (2018), Application(s): ELISA using human serum, Abstract; Full Text
Regulation of steroid hormones in the placenta and serum of women with preeclampsia: Y.Y. Shin, et al.; Mol. Med. Rep. 17, 2681 (2018), Application(s): Human placental tissue and blood serum, Abstract;
Tetragonia tetragonioides (Pall.) Kuntze regulates androgen production in a letrozole-induced polycystic ovary syndrome model: B.J. Pyun, et al.; Molecules 23, 1173 (2018), Application(s): ELISA using culture supernatants, Abstract; Full Text
Therapeutic Effect of Ecklonia cava Extract in Letrozole-Induced Polycystic Ovary Syndrome Rats: H. Yang, et al.; Front. Pharmacol. 9, 1325 (2018), Application(s): ELISA using medium supernatant of NCI-H295R cells, Abstract; Full Text
Consumption of green tea epigallocatechin-3-gallate enhances systemic immune response, antioxidative capacity and HPA axis functions in aged male swiss albino mice: R. Sharma, et al.; Biogerontology 18, 367 (2017), Application(s): Mouse plasma, Abstract;
Gender differences in susceptibility to schizophrenia: potential implication of neurosteroids: Y.C. Huang, et al.; Psychoneuroendocrinology 84, 87 (2017), Abstract;
Leukocyte profile, gene expression, acute phase response, and metabolite status of cows with sole hemorrhages: K. O'Driscoll, et al.; J. Dairy. Sci. 100, 9382 (2017), Application(s): ELISA using bovine serum, Abstract;
Upregulation of CYP17A1 by Sp1-mediated DNA demethylation confers temozolomide resistance through DHEA-mediated protection in glioma: J.Y. Chuang, et al.; Oncogenesis 6, e339 (2017), Abstract; Full Text
Increased Muscular 5α-Dihydrotestosterone in Response to Resistance Training Relates to Skeletal Muscle Mass and Glucose Metabolism in Type 2 Diabetic Rats: N. Horii, et al.; PLoS One 11, e0165689 (2016), Application(s): Plasma & skeletal muscle extracts, Abstract; Full Text
Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis: T. Escajadillo, et al.; Mol. Cell. Endrocrinol. 427, 73 (2016), Application(s): Measured DHEA levels, Abstract;
Responses of sex steroid hormones to different intensities of exercise in endurance athletes: K. Sato, et al.; Exp. Physiol. 101, 168 (2016), Application(s): DHEA quantification using human serum, Abstract;
Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers: K. O'Driscoll, et al.; J. Dairy Sci. 98, 1685 (2015), Application(s): DHEA quantification using bovine plasma, Abstract;
Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells: N.C. Lucki, et al.; Mol. Cell Endocrinol. 348, 165 (2012), Application(s): EIA using cell culture supernatants, Abstract; Full Text

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Datasheet
Assay Layout Sheet
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Certificate of Analysis
Manual
Absorbance96 Protocol File

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