Vaccines are a crucial tool used in public health to control the spread of infectious diseases. Historically, vaccines have been the single most effective means to fight and ultimately eradicate certain infectious diseases. Today, vaccines are in high demand as the global community struggles through epidemics of diseases that do not yet have a vaccine. We provide reagents to ensure that your vaccines are first-to-market and cost-effective, two key issues that can make or break the launch of a vaccine. As part of our portfolio, we offer tools to develop cell-based cancer vaccines, which efficiently target heterogenous cancer cells. Enzo Life Sciences offers a comprehensive offering of tools and forty years of expertise to ensure that your development is successful, from target identification to launch.
The AMPIPROBE® HCV Assay Kit is a (RT-qPCR) assay for the quantitative detection of human hepatitis C virus (HCV) RNA in plasma or serum. The proprietary primer mix included in the kit is specific for HCV genotypes 1 through 6.
The assay employs a novel in situ hybridization technique utilizing a cocktail of probes specific to multiple sites within the E6 and E7 genes to ensure the detection of these mRNA transcripts. This homogeneous assay can be performed in less than 3 hours and is specific for the most prevalent high-risk HPV genotypes.
Optimized Expression Systems Deliver Superior Antigenicity
We offer a broad range of native state bacterial and viral antigens for vaccine research and in vitro diagnostics markets. Purified native protein or production in the latest mammalian expression systems ensure that the resultant antigens are expressed to a high level, are in their native folded state, and possess all relevant post-translational modifications.
Detect Analytes Accurately with High-Sensitivity ELISA Kits
Our catalog of nearly 300 ELISA kits includes sensitive, specific assays for relevant markers of cell viability, signaling pathways, steroid and peptide hormones, inflammation, and more.
Detect Specific Cell Populations With Antibodies For CD Markers
We offer a broad range of antibodies for use in all areas of vaccine research. Many are validated for use in key applications such as flow cytometry, immunohistochemistry, immunoprecipitation, and Western blotting. Every antibody is backed by our Worry-free Antibody Trial Program, allowing you to evaluate any of our antibodies of interest for your specific application or species without risk.
High-activity, high-purity CD40L construct for co-stimulatory activation of an immune response.
Enhance Immune Activation with MEGACD40L® Protein
B cell lymphocyte activation by various CD40L constructs. PBMCs were treated for 48 hours in media containing serially diluted CD40L, CD40L + 2 μg/mL Enhancer, or MEGACD40L® Protein. Cells were dual-stained with α-human CD19–PE and α-human CD86–APC and analyzed by flow cytometry. Data are presented as the percent of CD19+ B cells that co-stain as CD86+.
Fas ligand with improved stability providing significantly enhanced immune activation.
Induction of Cell Death with SUPERFASLIGAND® Protein
Jurkat cells were incubated with the indicated concentration of SUPERFASLIGAND® and cell proliferation was assayed. Percent viability was determined in comparison to control wells with no SUPERFASLIGAND®.
With more than 3,000 biologically characterized small molecules and the industry’s most diverse collection of compound libraries, Enzo’s ultra-high purity compounds and proteins can be used to maximize yield via modulation of autophagy, proteases, and osmotic stress.
Monitoring Freeze-Thaw Cycle-Induced Aggregation in Bulk Proteins
Freeze-thaw cycle induced aggregation: 2 mg/mL of Rabbit anti-Goat IgG in 100 mM citrate, pH 2.7
Determining Optimal Storage pH for a Protein of Interest
The PROTEOSTAT® Thermal Shift Stability Assay can detect the optimal storage pH for a protein of interest. As illustrated, β-lactoglobulin was incubated with 40 mM buffer of pH ranging from 3 to 12 in the presence of 150 mM sodium chloride. At pH 7 and above, the stability of β-lactoglobulin decreases, as indicated by the lower aggregation temperature. Published studies have shown that the protein undergoes a conformational transition at this pH value (Blanch et al. (1997) Prot. Sci. 8:1362).
Recognizes All Commonly Used Protein A Constructs
Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery calculated by dividing observed concentration by expected concentration.
