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Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE®) (synthetic)

Activator of TLR3 and MDA5
ALX-746-021-M002 2 mg 157.00 USD
ALX-746-021-M005 5 mg 208.00 USD
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  • Specific activator of TLR3 and MDA5
  • Used to generate stable mature dendritic cells
  • Potential adjuvant in cancer vaccines
TLR3 is a key effector of the immune response against viruses by recognizing double-stranded RNA. Polyinosinic-polycytidylic acid (Poly(I:C)), a synthetic double-stranded homopolymer, binds TLR3 and activates the transcription factor interferon regulator factor 3 (IRF3) following the initiation of TIR domain-containing adaptor protein (TRIF)-dependent TLR signaling. Activation of IRF3 by poly(I:C) leads to the production of type I interferons. Poly(I:C) also activates the RNA helicases MDA-5 and RIG-1. Poly(I:C) can be used to generate stable mature dendritic cells in vitro and is considered a potent adjuvant in vaccine formulations, especially those targeting dendritic cells, thanks to the synergy between MDA5 and TLR3 activation.

Product Details

Alternative Name:poly(I:C) . K, poly(I:C)
Formula:(C10H10N4NaO7P)x • (C9H11N3NaO7P)x
Quantity:Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 10-25µg/ml.
Formulation:Lyophilized. Sterile.
Purity Detail:Activity and endotoxin tested - TLRGRADE®.
Endotoxin Content:<0.002EU/µg (LAL test; BioWhittaker)
Reconstitution:For a 1mg/ml stock solution, dissolve the total vial content in 2ml (ALX-746-021-M002) or 5ml (ALX-746-021-M005) endotoxin-free buffer or water. To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.
Shipping:Ambient Temperature
Long Term Storage:+4°C
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
Regulatory Status:RUO - Research Use Only
Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE) (synthetic) image
Figure 2: Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in mouse in vivo. Method: Poly(I:C) was injected i.v. and plasma levels of IFN α/β were determined 2 hours later by cytokine ELISA.
Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE) (synthetic) image
Figure 1: Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in murine BM-derived macrophages. Method: Poly(I:C) was added to bone marrow-derived murine macrophages in a 96-well plate at the indicated concentrations, cell supernatants were harvested after 24 hours and IFN α/β levels analyzed by cytokine ELISA.
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Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE) (synthetic) image Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE) (synthetic) image

Product Literature References

CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes: K. Rawat, et al.; J. Exp. Med. 220, e20222129 (2023), Abstract;
Nicotinamide mononucleotide (NMN) alleviates the poly(I:C)-induced inflammatory response in human primary cell cultures: H. Sano, et al.; Sci. Rep. 13, 11765 (2023), Abstract;
Synthetic circular RNA switches and circuits that control protein expression in mammalian cells: S. Kameda, et al.; Nucleic Acids Res. 51, e24 (2023), Abstract;
Function conservation and disparities of zebrafish and human LGP2 genes in fish and mammalian cells responsive to poly (I: C): X. Gong, et al.; Front. Immunol. 13, 985792 (2022), Abstract; Full Text
Human cDC1s express in-doleamine 2, 3-dioxygenase 1 (IDO) with functional effects on T cell priming: S.P. Sittig, et al.; Eur. J. Immunol. 51, 1494 (2021), Abstract;
IRF-8/miR-451a regulates M-MDSC differentiation via the AMPK/mTOR signal pathway during lupus development: G. Shi, et al.; Cell Death Discov. 7, 41420 (2021), Abstract;
N4BP1 negatively regulates NF-κB by binding and inhibiting NEMO oligomerization: H. Shi, et al.; Nat. Commun. 12, 1379 (2021), Abstract;
Role of extracellular microRNA-146a-5p in host innate immunity and bacterial sepsis: S. Wang, et al.; iScience 24, 103441 (2021), Abstract; Full Text
Time-Lapse Imaging of Necroptosis and DAMP Release at Single-Cell Resolution: S. Murai , et al.; Methods Mol. Biol. 2274, 353 (2021), Abstract;
TLR2- and TLR3-activated microglia induce different levels of neuronal network dysfunction in a context-dependent manner: S. Schilling, et al.; Brain Behav. Immun. 17, 889 (2021), Abstract;
Identification of the hallmarks of necroptosis and ferroptosis by transmission electron microscopy: S. Miyake, et al.; Biochem. Biophys. Res. Commun. 527, 839 (2020), Abstract;
Metabolic sialic acid blockade lowers the activation threshold of moDCs for TLR stimulation: C. Büll, et al.; Immunol. Cell Biol. 94, 408 (2017), Abstract;
Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity: J.J.P. van Beek, et al.; Oncoimmunology 5, e1227902 (2016), Application(s): Human Dendritic Cells stimulation, Abstract; Full Text
Enterovirus-Infected β-Cells Induce Distinct Response Patterns in BDCA1+ and BDCA3+ Human Dendritic Cells: B. M. Schulte, et al.; PLoS One 10, e0121670 (2015), Application(s): Cell Culture, Abstract; Full Text
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract;
Activation of murine macrophages via TLR2 and TLR4 is negatively regulated by a Lyn/PI3K module and promoted by SHIP1: S. Keck, et al.; J. Immunol. 184, 5809 (2010), Abstract; Full Text
Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity: M. Peiser, et al.; J. Leukoc. Biol. 83, 1118 (2008), Abstract;
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006), Abstract;
The dsRNA binding site of human Toll-like receptor 3: J.K. Bell, et al.; PNAS 103, 8792 (2006), Abstract;
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003), Abstract;

General Literature References

Noncanonical inflammasome activation by intracellular LPS independent of TLR4.: N. Kayagaki, et al.; Science 341, 1246 (2013), Abstract;

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