- Specific activator of TLR3 and MDA5
- Used to generate stable mature dendritic cells
- Potential adjuvant in cancer vaccines
TLR3 is a key effector of the immune response against viruses by recognizing double-stranded RNA. Polyinosinic-polycytidylic acid (Poly(I:C)), a synthetic double-stranded homopolymer, binds TLR3 and activates the transcription factor interferon regulator factor 3 (IRF3) following the initiation of TIR domain-containing adaptor protein (TRIF)-dependent TLR signaling. Activation of IRF3 by poly(I:C) leads to the production of type I interferons. Poly(I:C) also activates the RNA helicases MDA-5 and RIG-1. Poly(I:C) can be used to generate stable mature dendritic cells in vitro and is considered a potent adjuvant in vaccine formulations, especially those targeting dendritic cells, thanks to the synergy between MDA5 and TLR3 activation.
Product Details
| Alternative Name: | poly(I:C) . K, poly(I:C) |
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| Formula: | (C10H10N4NaO7P)x • (C9H11N3NaO7P)x |
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| Source: | Synthetic. |
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| CAS: | 31852-29-6 |
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| Quantity: | Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 10-25µg/ml. |
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| Formulation: | Lyophilized. Sterile. |
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| Purity Detail: | Activity and endotoxin tested - TLRGRADE®. |
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| Endotoxin Content: | <0.002EU/µg (LAL test; BioWhittaker) |
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| Reconstitution: | For a 1mg/ml stock solution, dissolve the total vial content in 2ml (ALX-746-021-M002) or 5ml (ALX-746-021-M005) endotoxin-free buffer or water. To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
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| Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No.
ALX-746-021) induces cytokine release in mouse
in vivo.
Method: Poly(I:C) was injected i.v. and plasma levels of IFN α/β were determined 2 hours later by cytokine ELISA.
Figure 1: Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No.
ALX-746-021) induces cytokine release in murine BM-derived macrophages.
Method: Poly(I:C) was added to bone marrow-derived murine macrophages in a 96-well plate at the indicated concentrations, cell supernatants were harvested after 24 hours and IFN α/β levels analyzed by cytokine ELISA.
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Product Literature References
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Application(s): Human Dendritic Cells stimulation,
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Full Text
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Full Text
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Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity: M. Peiser, et al.; J. Leukoc. Biol.
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Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature
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General Literature References
Noncanonical inflammasome activation by intracellular LPS independent of TLR4.: N. Kayagaki, et al.; Science
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