Specific activator for TLR3 (Toll-like receptor 3) and MDA5/Helicard.
Product Details
| Alternative Name: | poly(I:C) . K |
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| Formula: | (C10H10N4NaO7P)x • (C9H11N3NaO7P)x |
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| Source: | Synthetic. |
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| CAS: | 31852-29-6 |
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| Quantity: | Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 10-25µg/ml. |
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| Formulation: | Lyophilized. Sterile. |
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| Purity Detail: | Activity and endotoxin tested - TLRGRADE®. |
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| Endotoxin Content: | <0.002EU/µg (LAL test; BioWhittaker) |
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| Reconstitution: | For a 1mg/ml stock solution, dissolve the total vial content in 2ml (ALX-746-021-M002) or 5ml (ALX-746-021-M005) endotoxin-free buffer or water. To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving. |
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| Shipping: | Ambient |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
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| Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No.
ALX-746-021) induces cytokine release in mouse
in vivo.
Method: Poly(I:C) was injected i.v. and plasma levels of IFN α/β were determined 2 hours later by cytokine ELISA.
Figure 1: Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No.
ALX-746-021) induces cytokine release in murine BM-derived macrophages.
Method: Poly(I:C) was added to bone marrow-derived murine macrophages in a 96-well plate at the indicated concentrations, cell supernatants were harvested after 24 hours and IFN α/β levels analyzed by cytokine ELISA.
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Product Literature References
Human cDC1s express in-doleamine 2, 3-dioxygenase 1 (IDO) with functional effects on T cell priming: S.P. Sittig, et al.; Eur. J. Immunol.
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N4BP1 negatively regulates NF-κB by binding and inhibiting NEMO oligomerization: H. Shi, et al.; Nat. Commun.
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Time-Lapse Imaging of Necroptosis and DAMP Release at Single-Cell Resolution: S. Murai , et al.; Methods Mol. Biol.
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TLR2- and TLR3-activated microglia induce different levels of neuronal network dysfunction in a context-dependent manner: S. Schilling, et al.; Brain Behav. Immun.
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Metabolic sialic acid blockade lowers the activation threshold of moDCs for TLR stimulation: C. Büll, et al.; Immunol. Cell Biol.
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Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity: J.J.P. van Beek, et al.; Oncoimmunology
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Application(s): Human Dendritic Cells stimulation,
Abstract;
Full Text
Enterovirus-Infected β-Cells Induce Distinct Response Patterns in BDCA1+ and BDCA3+ Human Dendritic Cells: B. M. Schulte, et al.; PLoS One
10, e0121670 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun.
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Abstract;
Activation of murine macrophages via TLR2 and TLR4 is negatively regulated by a Lyn/PI3K module and promoted by SHIP1: S. Keck, et al.; J. Immunol.
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Abstract;
Full Text
Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity: M. Peiser, et al.; J. Leukoc. Biol.
83, 1118 (2008),
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Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature
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Abstract;
The dsRNA binding site of human Toll-like receptor 3: J.K. Bell, et al.; PNAS
103, 8792 (2006),
Abstract;
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol.
171, 3154 (2003),
Abstract;
General Literature References
Noncanonical inflammasome activation by intracellular LPS independent of TLR4.: N. Kayagaki, et al.; Science
341, 1246 (2013),
Abstract;
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