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IL-8 (human), ELISA kit

Ultrasensitive ELISA, measuring as low as 1 pg/ml of IL-8 in just 3 hours
ADI-900-156A 96 wells 658.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ADI-900-156

Alternative sizes available: ADI-901-156A (5x96 wells)
  • Ultrasensitive measurement, < 1 pg/ml IL-8
  • Fast results < 3 hours from up to 37 samples in duplicate
  • Reproducible and fully validated in-house in a variety of sample types
  • Ready-to-use reagents and pre-coated plates reduce errors and save your time
The IL-8 (human), ELISA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Ultrasensitive measurement of < 1 pg/mL IL-8 with excellent lot-to-lot reproducibility.
IL-8 (human), ELISA kit standard curve
Figure 1. Typical Standard Curve for ADI-900-156A
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IL-8 (human), ELISA kit standard curve

Product Details

Alternative Name:Interleukin-8
Sensitivity:0.72 pg/ml (range 7.8-1,000 pg/ml)
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human IL-8 in culture supernatants, plasma, and serum.
Wavelength:450 nm
Species reactivity:Human
Use/Stability:Store all components at +4°C, except standard at -20°C.
Shipping:Dry Ice and Blue Ice
Contents:Microtiter Plate, Conjugate, Antibody, Assay Buffer 13, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Assay Layout Sheet, Plate Sealer
Scientific Background:Interleukin-8 (IL-8) is a cytokine produced by monocytes, macrophages, neutrophils, lymphocytes, dermal fibroblasts, keratinocytes, vascular endothelial cells, melanocytes, hepatocytes and various tumor cell lines. It is also a member of the CXC family of chemokines, displaying chemotactic activity for neutrophils and T cells.
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
UniProt ID:P10145
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal

Product Literature References

Exploring association of melanoma-specific Bcl-xL with tumor immune microenvironment: A.M. Lucianò, et al.; J. Exp. Clin. Cancer Res. 42, 178 (2023), Abstract;
Profiling Distinctive Inflammatory and Redox Responses to Hydrogen Sulfide in Stretched and Stimulated Lung Cells: S.G. Spassov, et al.; Antioxidants 11, 1001 (2022), Abstract;
Responses of increasingly complex intestinal epithelium in vitro models to bacterial toll-like receptor agonists: M. Grouls, et al.; Toxicol. In Vitro 79, 105280 (2021), Abstract;
25-OH vitamin D and interleukin-8: emerging biomarkers in cutaneous melanoma development and progression: C.D. Ene, et al.; Mediators Inflamm. 2015, 904876 (2015), Application(s): ELISA using human serum, Abstract; Full Text
Prolonged and repetitive exposure to Porphyromonas gingivalis increases aggressiveness of oral cancer cells by promoting acquisition of cancer stem cell properties: N.H. Ha, et al.; Tumour Biol. 36, 9947 (2015), Abstract;
Unsaturated fatty acids induce mesenchymal stem cells to increase secretion of angiogenic mediators: A.N. Smith, et al.; J. Cell. Physiol. 227, 3225 (2012), Application(s): EIA using culture supernatants from human bone marrow-derived mesenchymal cells, Abstract;
Including indigestible carbohydrates in the evening meal of healthy subjects improves glucose tolerance, lowers inflammatory markers, and increases satiety after a subsequent standardized breakfast: A. Nilsson, et al. ; J. Nutr. 138, 732 (2008), Application(s): EIA using human serum, Abstract;

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