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Better Development Upstream
to Ensure Quality Downstream

  • Protein Aggregation
  • Thermal Shift Stability

  • Protein A
  • Host Cell Protein

  • PEGylated Proteins

  • Simplify your cell death analysis
  • Sensitive assays for oxidative stress
  • Reliable detection of cell line instability
  • Small Molecules for Bioprocessing Applications

Innovative Tools for Biotherapeutics Development

Development of a single drug, whether it is a new chemical entity, biotherapeutic, or genetic/cellular therapy, requires significant investment of resources. Each step of the process from early discovery through production and delivery must be fully explored, characterized, and understood to prevent issues resulting from cell stress, cell death, protein aggregation, and factors affecting reliable manufacturing of the drug. Whether you work in drug discovery, upstream, or downstream bioprocessing, Enzo offers a range of products to help you maintain cell line viability, optimize and monitor product integrity, and maximize yield.

Featured Tutorial

Optimizing Biologics Formulations : Quantifying Trace Subvisible Protein Aggregates Using a Protein Aggregation Assay and Standards.”
Wayne F. Patton, Ph.D.
Read the tutorial
Small Molecules ELISA and Detection Kits
Osmotic Stress Modulators Oxidative Stress
Hybridoma Modulators Cell Death: Autophagy, Apoptosis, Necrosis
Autophagy Inhibitors Cell Line Instability
Compound Libraries IgG ELISA Kits
Toxicity Contamination Control (Protein A & Host Cell Proteins)
Protease Inhibitors PEGylated Protein
Bioactives Thermal Shift (Stability)
Hybridoma Production Refolding
DiSH Kit Protein Aggregates
CHO Semi-Solid Media Biosimilar Conformational ELISAs

Guide to Optimizing Protein Characterization

  • Current Food and Drug Administration Guidelines
  • How to Determine Protein Stability
  • Methods for Detecting Protein Aggregates
  • Development and Specificity of the PROTEOSTAT® Dye
  • Using PROTEOSTAT® for a Rapid Detection and Characterization of Protein Aggregates by Flow Cytometry
  • Detection of Bacterial Aggregates by Flow Cytometry
  • Using PROTEOSTAT® Reagents to Predict Aggregation
  • Propensity and Monitor Aggregation of Antibody-Drug Conjugates (ADC)

Optimize and Monitor Product Integrity

PROTEOSTAT® Protein Aggregation Assay

The PROTEOSTAT® Protein Aggregation Assay is a simple, sensitive, homogeneous fluorescent microplate assay. The novel molecular rotor dye used detects a broad range of different protein aggregates from visible to subvisible particles.
  • Delivers superior results when compared to conventional protein detection dyes (Sypro Orange, Thioflavin T, ANS, Congo Red)
  • Works over a wide pH and ionic strength range and is compatible with commonly used buffers and excipients
  • Provides a convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
  • Useful assay to troubleshoot protein damage, from protein development through manufacturing stages

Related Product
PROTEOSTAT® Protein Aggregation Standards

Reference articles

Optimizing Biologics Formulations
Here and There on Upstream Processing

PROTEOSTAT® Thermal Shift Stability Assay

Based on an innovative molecular rotor dye, the PROTEOSTAT® Thermal Shift Stability Assay is a rapid, simple, and sensitive screening method allowing the end user to investigate the aggregation propensity of proteins by determing the temperature of aggregation.

Related Product
PROTEOSTAT® Protein Refolding & Aggregation Sensing Kit

Reference articles

Here and There on Upstream Processing

  • Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
  • Screen for ligand binding to proteins of unknown function
  • Screen protein variants to determine their relative stabilities or excipients to determine optimal protein storage conditions
  • Identify protein-protein interaction inhibitors

Contamination Monitoring

Protein A ELISA Kit

The Protein A ELISA kit is a sensitive and reproducible sandwich assay to quantify Protein A residuals in monoclonal antibody preparations. This extensively validated ELISA kit enables efficient detection of natural and recombinant Protein A constructs with up to 100% recovery.
  • Detects 1ppm of Protein A residuals in human IgG
  • Recognizes 4 different Protein A variants
  • Useful for contamination analysis and measurement of Protein A variants in monoclonal antibody preparations
  • Produces results in less than 3 hours with low cost per test

Host Cell Protein ELISA Kits

The Host Cell Protein ELISA kits and reagents are ideal for impurity analysis, Host Cell Protein quantitation and Western blots.

  • Quantitatively measures host cell protein contamination in E.Coli and CHO derived biologics
  • High-throughput format with results in 2.5 hours
  • High protein coverage

Improved Screening for Biotherapeutic Candidates

Improve Pharmacokinetic Analysis - PEGylated Protein ELISA Kit

The sensitive PEGylated Protein ELISA kit is ideal for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies.
  • Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins
  • Sensitive assay measures < 1ng/ml of PEGylated molecules
  • Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue
  • High throughput format allows analysis of up to 35 samples in duplicate in just 2 hours

Related Products
Methotrexate ELISA Kit
Protein Carbonyl ELISA Kit
Transferrin ELISA Kit

Sensitive detection of linear and branched PEG molecules

Determine Organ-Specific Toxicity - SCREEN-WELL® Toxicity Libraries

Targeted SCREEN-WELL® libraries for cardiotoxicity, hepatotoxicity, hematopoietic toxicity, nephrotoxicity and myotoxicity contain compounds with defined and diverse organ-associated toxicity profiles. A variety of structurally and mechanistically different compound classes are included, as well as non-toxic controls. Compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates at 100 or 500 μL per well. The libraries are useful for predictive toxicology screening, including high-content protocols.
  • Better understand mechanisms of toxicity with your small molecule or candidate-drug of interest
  • Compare your compound with known references for investigative toxicity
  • Optimize formulation efficacy and safety

Complete Solutions for Process Optimization

Simplify Your Cell Death Analysis

CYTO-ID® Autophagy Detection Kit 2.0

  • No transfection required
  • Brighter, more photostable dye specifically stains autophagic vesicles
  • Rapidly quantifies autophagy in native heterogeneous cell populations
  • Negligible staining of lysosomes reduces background

GFP-CERTIFIED® Apoptosis/Necrosis Detection Kit

  • True multiplexing capabilities with GFP and other green fluorescent probes
  • Readily distinguishes between healthy, early apoptotic, late apoptotic, and necrotic cells
  • Optimized for both fluorescence microscopy and flow cytometry

Sensitive Assays For Oxidative Stress

ROS-ID® Total ROS/Superoxide Detection Kit

  • Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxly radicals
  • High sensitivity, specificity and accuracy for live cell studies
  • Complete set of regents, including ROS inducers and scavengers
  • Validated on microscopy, flow cytometry, and microplates

MITO-ID® Membrane Potential Cytotoxicity Assay Kit

  • 10x more sensitive than JC-1 with superior aqueous solubility
    • Photostable dual-emission dye
        Separate MITO-ID® Red/Green assays available for detection of mitochondrial mass
          Suitable for high-throughput applications

Bioactives For Drug Development

Ultra-high Purity | Cost Effective | Extensively Validated

  • Compounds available for resupply in bulk
  • Full mechanism of action files available

Cell Culture Supplements
Caspase Inhibitors
HDAC Inhibitors
Protease Inhibitors

Absorbance 96 Plate Reader Plate Readers Just Got Personal

  • Perfect for ELISA Kits
  • Endpoint and kinetic assays
  • Quantitative and qualitative evaluation
  • Custom protocols and reports

Related Literature and Documents

Featured TechNotes

Are biosimilars really similar?
2014 October 3 | Tags: Bioprocess

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