Better Development Upstream
to Ensure Quality Downstream

Protein Aggregation
Thermal Shift Stability

Protein A
Host Cell Protein

PEGylated Proteins

Simplify your cell death analysis
Sensitive assays for oxidative stress
Reliable detection of cell line instability
Small Molecules for Bioprocessing Applications

Innovative Tools for Biotherapeutics Development

Development of a single drug, whether it is a new chemical entity, biotherapeutic, or genetic/cellular therapy, requires significant investment of resources. Each step of the process from early discovery through production and delivery must be fully explored, characterized, and understood to prevent issues resulting from cell stress, cell death, protein aggregation, and factors affecting reliable manufacturing of the drug. Whether you work in drug discovery, upstream, or downstream bioprocessing, Enzo offers a range of products to help you maintain cell line viability, optimize and monitor product integrity, and maximize yield.

Featured Tutorial

Optimizing Biologics Formulations : Quantifying Trace Subvisible Protein Aggregates Using a Protein Aggregation Assay and Standards.”
Wayne F. Patton, Ph.D.
Read the tutorial

Small Molecules		ELISA and Detection Kits
Osmotic Stress Modulators		Oxidative Stress
Hybridoma Modulators		Cell Death: Autophagy, Apoptosis, Necrosis
Autophagy Inhibitors		Cell Line Instability
Compound Libraries		IgG ELISA Kits
Toxicity		Contamination Control (Protein A & Host Cell Proteins)
Protease Inhibitors		PEGylated Protein
Bioactives		Thermal Shift (Stability)
Hybridoma Production		Refolding
DiSH Kit		Protein Aggregates
CHO Semi-Solid Media		Biosimilar Conformational ELISAs

Guide to Optimizing Protein Characterization

Current Food and Drug Administration Guidelines
How to Determine Protein Stability
Methods for Detecting Protein Aggregates
Development and Specificity of the PROTEOSTAT® Dye
Using PROTEOSTAT® for a Rapid Detection and Characterization of Protein Aggregates by Flow Cytometry
Detection of Bacterial Aggregates by Flow Cytometry
Using PROTEOSTAT® Reagents to Predict Aggregation
Propensity and Monitor Aggregation of Antibody-Drug Conjugates (ADC)

Optimize and Monitor Product Integrity

PROTEOSTAT® Protein Aggregation Assay

The PROTEOSTAT® Protein Aggregation Assay is a simple, sensitive, homogeneous fluorescent microplate assay. The novel molecular rotor dye used detects a broad range of different protein aggregates from visible to subvisible particles.

Delivers superior results when compared to conventional protein detection dyes (Sypro Orange, Thioflavin T, ANS, Congo Red)
Works over a wide pH and ionic strength range and is compatible with commonly used buffers and excipients
Provides a convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
Useful assay to troubleshoot protein damage, from protein development through manufacturing stages

Related Product
PROTEOSTAT® Protein Aggregation Standards

Reference articles

Optimizing Biologics Formulations
Here and There on Upstream Processing

PROTEOSTAT® Thermal Shift Stability Assay

Based on an innovative molecular rotor dye, the PROTEOSTAT® Thermal Shift Stability Assay is a rapid, simple, and sensitive screening method allowing the end user to investigate the aggregation propensity of proteins by determing the temperature of aggregation.

Reference articles

Here and There on Upstream Processing

Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
Screen for ligand binding to proteins of unknown function
Screen protein variants to determine their relative stabilities or excipients to determine optimal protein storage conditions
Identify protein-protein interaction inhibitors

Contamination Monitoring

Protein A ELISA Kit

The Protein A ELISA kit is a sensitive and reproducible sandwich assay to quantify Protein A residuals in monoclonal antibody preparations. This extensively validated ELISA kit enables efficient detection of natural and recombinant Protein A constructs with up to 100% recovery.

Detects 1ppm of Protein A residuals in human IgG
Recognizes 4 different Protein A variants
Useful for contamination analysis and measurement of Protein A variants in monoclonal antibody preparations
Produces results in less than 3 hours with low cost per test

Host Cell Protein ELISA Kits

The Host Cell Protein ELISA kits and reagents are ideal for impurity analysis, Host Cell Protein quantitation and Western blots.

Quantitatively measures host cell protein contamination in E.Coli and CHO derived biologics
High-throughput format with results in 2.5 hours
High protein coverage

Improved Screening for Biotherapeutic Candidates

Improve Pharmacokinetic Analysis - PEGylated Protein ELISA Kit

The sensitive PEGylated Protein ELISA kit is ideal for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies.

Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins
Sensitive assay measures < 1ng/ml of PEGylated molecules
Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue
High throughput format allows analysis of up to 35 samples in duplicate in just 2 hours

Related Products
Methotrexate ELISA Kit
Protein Carbonyl ELISA Kit
Transferrin ELISA Kit

Sensitive detection of linear and branched PEG molecules

Determine Organ-Specific Toxicity - SCREEN-WELL® Toxicity Libraries

Targeted SCREEN-WELL® libraries for cardiotoxicity, hepatotoxicity, hematopoietic toxicity, nephrotoxicity and myotoxicity contain compounds with defined and diverse organ-associated toxicity profiles. A variety of structurally and mechanistically different compound classes are included, as well as non-toxic controls. Compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates at 100 or 500 μL per well. The libraries are useful for predictive toxicology screening, including high-content protocols.

Better understand mechanisms of toxicity with your small molecule or candidate-drug of interest
Compare your compound with known references for investigative toxicity
Optimize formulation efficacy and safety

Complete Solutions for Process Optimization

Simplify Your Cell Death Analysis

CYTO-ID® Autophagy Detection Kit 2.0

No transfection required
Brighter, more photostable dye specifically stains autophagic vesicles
Rapidly quantifies autophagy in native heterogeneous cell populations
Negligible staining of lysosomes reduces background

GFP-CERTIFIED® Apoptosis/Necrosis Detection Kit

True multiplexing capabilities with GFP and other green fluorescent probes
Readily distinguishes between healthy, early apoptotic, late apoptotic, and necrotic cells
Optimized for both fluorescence microscopy and flow cytometry

Sensitive Assays For Oxidative Stress

ROS-ID® Total ROS/Superoxide Detection Kit

Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxly radicals
High sensitivity, specificity and accuracy for live cell studies
Complete set of regents, including ROS inducers and scavengers
Validated on microscopy, flow cytometry, and microplates