• Delivers superior results when compared to conventional protein detection dyes (Sypro Orange, Thioflavin T, ANS, Congo Red)
• Works over a wide pH and ionic strength range and is compatible with commonly used buffers and excipients
• Provides a convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
• Useful assay to troubleshoot protein damage, from protein development through manufacturing stages

Reference articles

Here and There on Upstream Processing

• Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
• Screen for ligand binding to proteins of unknown function
• Screen protein variants to determine their relative stabilities or excipients to determine optimal protein storage conditions
• Identify protein-protein interaction inhibitors
• ProteoStat® dye is tolerant of detergents and hydrophobic compounds

• Detects 1ppm of Protein A residuals in human IgG
• Recognizes 4 different Protein A variants
• Useful for contamination analysis and measurement of Protein A variants in monoclonal antibody preparations
• Produces results in less than 3 hours with low cost per test

• Quantitatively measures host cell protein contamination in E.Coli and CHO derived biologics
• Manufactured under cGMP to ensure quality and consistency
• High throughput format with results in 2.5 hours

• Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins
• Sensitive assay measures < 1ng/ml of PEGylated molecules
• Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue
• High throughput format allows analysis of up to 35 samples in duplicate in just 2 hours

Cell Death

Simplify your Cell Death Analysis

Enzo provides a variety of tools to detect the onset of cell death pathways, including sensitive fluorescent probes for detection of apoptosis, necrosis, and autophagy. These assays can help detect subtle changes in the health of adherent or suspension cell cultures used for production of biotherapeutics, and assess toxicity during drug development.

Autophagy and Apoptosis Assays

Oxidative Stress

Sensitive Assays for Oxidative Stress

Changes in mitochondrial function and glucose metabolism can adversely affect cell line viability, decreasing protein yield in cultured cells and long-term efficacy of cell based therapies. Enzo provides an industry-leading portfolio of kits and reagents for monitoring oxidative stress, including sensitive, high-throughput compatible assays for hypoxia, reactive oxygen species (ROS) and mitochondrial dysfunction.

High-throughput analysis of mitotoxicity

Cell Line Instability

Reliable Detection of Cell Line Instability

Changes in gene expression due to endogenous or transgene copy number loss, or epigenetic suppression can affect cell line stability and yield. Our portfolio of products for detection of cell line instability include fluorescent labeling kits for high-resolution array CGH, RNA amplification and labeling, and DNA methylation.

Superior dye incorporation results in more accurate detection

• Ultra-high purity
• Cost-effective
• Extensively validated