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Irreversible inhibitor
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Cell-permeable
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High purity peptide
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Non-cytotoxic
Irreversible, cell permeable broad-spectrum caspase inhibitor.
Product Details
| Alternative Name: | Caspase family inhibitor (fluoromethylketone), Z-Val-Ala-DL-Asp-FMK |
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| Sequence: | Z-Val-Ala-Asp-fluoromethylketone |
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| Formula: | C21H28FN3O7 |
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| MW: | 453.5 |
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| CAS: | 220644-02-0 |
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| Peptide Content: | 77-97% |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Solid |
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| Application Notes: | Can be used as a broad spectrum caspase inhibitor in conjunction with apoptosis detection assays. |
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| Solubility: | Soluble in DMSO or acetonitrile (10mg/ml). |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Keep sealed after removing from the freezer until its temperature equilibrates with room temperature. Dissolve only the amount needed at one time because of the possibility of decomposition in solution. |
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| Handling: | After reconstitution, prepare aliquots and store at -20°C. |
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| Protocol: | Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO. Add 2µl of this stock solution to 1ml of culture medium containing cells to give 20µM final concentration. Effective final concentrations are estimated to be 5-20µM. If a particular caspase inhibitor is applied at higher concentrations specificity for an individual caspase or even for the caspase family (> 100µM) might be compromised. DMSO concentration above 0.2% may cause cellular toxicity thus masking the effect of the caspase inhibitor. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease. |
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| Regulatory Status: | RUO - Research Use Only |
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Anti-tumor effects of an ID antagonist with no observed acquired resistance: P.M. Wojnarowicz, et al.; NPJ Breast Cancer
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Betulin, a Newly Characterized Compound in Acacia auriculiformis Bark, Is a Multi-Target Protein Kinase Inhibitor: A.A. Ahmadu, et al.; Molecules
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Induction of interferon-β and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells: V. Granqvist, et al.; PLoS One
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Advanced age protects resistance arteries of mouse skeletal muscle from oxidative stress through attenuating apoptosis induced by hydrogen peroxide: C.E. Norton, et al.; J. Physiol.
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Chemical proteasome inhibition as a novel animal model of inner retinal degeneration in rats: M. Kageyama, et al.; PLoS One
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CIB1 depletion with docetaxel or TRAIL enhances triple-negative breast cancer cell death: A. H. Chung, et al.; Cancer Cell Int.
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Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria: H.P. Cudré-Cung, et al.; Mol. Genet. Metab.
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Application(s): Cell culture, blocking apoptosis,
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Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice: M.O. Grootaert, et al.; Oxid. Med. Cell Longev.
2016, 3087 (2016),
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Full Text
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Application(s): Blocked increased caspase-3 activity,
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Full Text
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Abstract;
Full Text
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Full Text
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Abstract;
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283, 168 (2015),
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5, 140156 (2015),
Application(s): Cell Culture,
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Full Text
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16, 289449 (2015),
Application(s): Blocked transcription,
Abstract;
Full Text
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6, e1691 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
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Application(s): Cell Culture,
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Full Text
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Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression: F. Zhong, et al.; Oncol. Lett.
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Full Text
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11, e1004887 (2015),
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Full Text
The Tpl2 kinase regulates the COX-2/prostaglandin E2 axis in adipocytes in inflammatory conditions: F. Berthou, et al.; Mol. Endocrinol.
29, 1025 (2015),
Application(s): Cell Culture,
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Full Text
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290, 10905 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
USP30 deubiquitylates mitochondrial Parkin substrates and restricts apoptotic cell death: J.R. Liang, et al.; EMBO Rep.
16, 618 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
TLR4 Activation Under Lipotoxic Conditions Leads to Synergistic Macrophage Cell Death Through a TRIF-Dependent Pathway: J. D. Schilling et al.; J. Immunol.
190, 1285 (2013),
Application(s): Inhibition of caspase in macrophages,
Abstract;
Full Text
Internalization and induction of antioxidant messages by microvesicles contribute to the antiapoptotic effects on human endothelial cells: R. Soleti, et al.; Free Radic. Biol. Med.
53, 2159 (2012),
Application(s): HUVEC cell culture,
Abstract;
Cytotoxicity of troglitazone through PPARγ-independent pathway and p38 MAPK pathway in renal cell carcinoma: M. Fujita, et al.; Cancer Lett.
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Inhibition of poly(ADP-ribose) polymerase (PARP) induces apoptosis in lung cancer cell lines: N.N. Gangopadhyay, et al.; Cancer Invest.
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Type of cell death induced by various metal cations in cultured human gingival fibroblasts: R.G. Contreras, et al.; In Vivo
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Neuroprotection by a caspase inhibitor in acute bacterial meningitis: J.S. Braun, et al.; Nat. Med.
5, 298 (1999),
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An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells: H. Zhu, et al.; FEBS Lett.
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Abstract;
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