25-Hydroxyvitamin D3 is an Intermediate in the biosynthesis of 1α,25-dihydroxyvitamin D3 and a major circulating metabolite of vitamin D3. Plasma or serum levels of 25-hydroxyvitamin D3 can be determined by ELISA (Prod. No. ADI-900-215) and used as indicators of the vitamin D status of an individual.
Product Details
Alternative Name:
Calcifediol, 25-Hydroxycholecalciferol
Formula:
C27H44O2
MW:
400.6
Source:
Synthetic.
CAS:
19356-17-3
Purity:
≥98% (HPLC)
Identity:
Identity determined by MS and NMR.
Appearance:
White to off-white crystalline solid.
Solubility:
Soluble in 100% ethanol.
Shipping:
Ambient
Long Term Storage:
-20°C
Handling:
Protect from light and oxygen.
Regulatory Status:
RUO - Research Use Only
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Product Literature References
Application of Bio-Active Elastin-like Polypeptide on Regulation of Human Mesenchymal Stem Cell Behavior: V. Sarangthem, et al.; Biomedicines 10, 1151 (2022), Abstract;
Autocrine vitamin D signaling switches off pro-inflammatory programs of TH1 cells: D. Chauss, et al.; Nat. Immunol. 23, 62 (2022), Abstract;
Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite: A. Mendoza, et al.; Cell Chem. Biol. 29, 660 (2022), Abstract;
Impaired Vitamin D Signaling in T Cells From a Family With Hereditary Vitamin D Resistant Rickets: F.A.H. Al-Jaberi, et al.; Front. Immunol. 12, 684015 (2021), Abstract; Full Text
CD55 regulates bone mass in mice by modulating RANKL-mediated Rac signaling and osteoclast function: B. Shin, et al.; J. Bone Miner. Res. 35, 130 (2020), Abstract; Full Text
Vitamin D promotes trophoblast cell induced separation of vascular smooth muscle cells in vascular remodeling via induction of G-CSF: J.Y. Zhang, et al.; Front Cell Dev Biol 8, 601043 (2020), Abstract; Full Text
Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis: B.D. Bryson, et al.; Nat. Commun. 10, 2329 (2019), Abstract; Full Text
Intrinsic activation of the vitamin D antimicrobial pathway by M. leprae infection is inhibited by type I IFN: K. Zavala, et al.; PLoS Negl. Trop. Dis. 12, e0006815 (2018), Abstract; Full Text
Role of Vitamin D in Maintaining Renal Epithelial Barrier Function in Uremic Conditions: M. Mihajlovic, et al.; Int. J. Mol. Sci. 18, 2531 (2017), Abstract; Full Text
Vitamin D Counteracts Mycobacterium tuberculosis-Induced Cathelicidin Downregulation in Dendritic Cells and Allows Th1 Differentiation and IFNγ Secretion: A.K.O. Rode, et al.; Front. Immunol. 8, 656 (2017), Abstract; Full Text
Identification and characterization of a vitamin D3 decomposition product bactericidal against Helicobacter pylori: K. Hosoda, et al.; Sci. Rep. 5, 8860 (2015), Abstract; Full Text
Vitamin D promotes human extravillous trophoblast invasion in vitro: S.Y. Chan, et al.; Placenta 36, 403 (2015), Application(s): Cell Culture, Abstract;
Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells: M. Kongsbak, et al.; PLoS One 9, e96695 (2014), Abstract;
Vitamin D-binding protein controls T cell responses to vitamin D: M. Kongsbak, et al.; BMC Immunol. 15, 35 (2014), Abstract; Full Text
25-hydroxyvitamin D3 suppresses hepatitis C virus production: T. Matsumura, et al.; Hepatology 56, 1231 (2012), Application(s): Treatment of HCV-infected liver cells, Abstract;
Effect of 25-hydroxyvitamin D3 and 1α,25 dihydroxyvitamin D3 on differentiation and apoptosis of human osteosarcoma cell lines: L. Thompson, et al.; J. Orthop. Res. 30, 831 (2012), Application(s): Treatment of human osteosarcoma cell lines, Abstract;
Vitamin D metabolites and analogs induce lipoxygenase mRNA expression and activity as well as reactive oxygen species (ROS) production in human bone cell line: D. Somjen, et al.; J. Steroid. Biochem. Mol. Biol. 123, 85 (2011), Application(s): Treatment of human bone cells, Abstract;
General Literature References
Enzyme immunoassay for plasma 25-hydroxyvitamin D3 employing immunoaffinity chromatography as a pretreatment method: N. Kobayashi, et al.; J.Steroid Biochem. Mol. Biol. 44, 93 (1993),
Evidence for human placental synthesis of 24,25-dihydroxyvitamin D3 and 23,25-dihydroxyvitamin D3: L.P Rubin, et al.; Pediatr. Res. 34, 98 (1993),
