Synthetic peptide that irreversibly inhibits ICE-family protease/caspase activity and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability.
Product Details
| Alternative Name: | Caspase family inhibitor (fluoromethylketone) |
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| Sequence: | Z-Val-Ala-Asp-fluoromethylketone |
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| Formula: | C22H30FN3O7 |
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| MW: | 467.5 |
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| Source: | Synthetic |
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| Formulation: | Liquid. In DMSO. |
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| Purity: | ≥99% (TLC) |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | We recommend a final concentration of 2-10µM for inhibiting caspase activity in Jurkat cell culture. The optimal doses may vary for different cells and culture conditions. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Apoptosis was induced in Jurkat cells with camptothecin (2µM) for 6 hours in the presence of various concentrations of Z-VAD-FMK (Prod. No.
ALX-260-138). Caspase activity was measured using Caspase-3 Fluorometric Assay Kit (Prod. No.
ALX-850-216) according to the kit instructions."
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Product Literature References
Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor-induced senescence in breast cancer cells: I.D. Kartika, et al.; Cancer Med.
10, 8988 (2021),
Abstract;
Rapid Release of Interleukin-1β from Human Platelets Is Independent of NLRP3 and Caspase: G.J. Pennings, et al.; Thromb. Haemost. (2021),
Abstract;
Ferroptosis in Neurons and Cancer Cells Is Similar But Differentially Regulated by Histone Deacetylase Inhibitors: M. Zille, et al.; eNeuro
6, ENEURO.0263-18.2019 (2019),
Abstract;
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Coordination of receptor tyrosine kinase signaling and interfacial tension dynamics drive radial intercalation and tube elongation: N. Neumann, et al.; Dev. Cell
45, 67 (2018),
Abstract;
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Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells: N. Pizato, et al.; Sci. Rep.
8, 1952 (2018),
Abstract;
Lysosomal Degradation of CD44 Mediates Ceramide Nanoliposome-induced Anoikis and Diminished Extravasation in Metastatic Carcinoma Cells: J. K. Haakenson, et al.; J. Biol. Chem.
290, 8632 (2015),
Application(s): Cell Culture ,
Abstract;
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General Literature References
Baculovirus p35 and Z-VAD-fmk inhibit thapsigargin-induced apoptosis of breast cancer cells: X.M. Qi, et al.; Oncogene
15, 1207 (1997),
Abstract;
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J.
315, 21 (1996),
Abstract;
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Fas-induced activation of the cell death-related protease CPP32 Is inhibited by Bcl-2 and by ICE family protease inhibitors: R.C. Armstrong, et al.; J. Biol. Chem.
271, 16850 (1996),
Abstract;
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