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Z-VAD-FMK (Ready-to-Use)

Caspase inhibitor
ALX-260-138-R100 100 µl 2mM Inquire for pricing
ALX-260-138-R020 20 µl 10mM 250.00 USD
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Synthetic peptide that irreversibly inhibits ICE-family protease/caspase activity and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability.

Product Details

Alternative Name:Caspase family inhibitor (fluoromethylketone)
Formulation:Liquid. In DMSO.
Purity:≥99% (TLC)
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:We recommend a final concentration of 2-10µM for inhibiting caspase activity in Jurkat cell culture. The optimal doses may vary for different cells and culture conditions.
Regulatory Status:RUO - Research Use Only
Z-VAD-FMK (Ready-to-Use) image
Figure: Apoptosis was induced in Jurkat cells with camptothecin (2µM) for 6 hours in the presence of various concentrations of Z-VAD-FMK (Prod. No. ALX-260-138). Caspase activity was measured using Caspase-3 Fluorometric Assay Kit (Prod. No. ALX-850-216) according to the kit instructions."
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Z-VAD-FMK (Ready-to-Use) image

Product Literature References

Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress: M.B. Wachte, et al.; Life Sci. Alliance 6, e202201494 (2023), Abstract;
Rapid Release of Interleukin-1β from Human Platelets Is Independent of NLRP3 and Caspase: G.J. Pennings, et al.; Thromb. Haemost. 122, 517 (2022), Abstract;
Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor-induced senescence in breast cancer cells: I.D. Kartika, et al.; Cancer Med. 10, 8988 (2021), Abstract;
Ferroptosis in Neurons and Cancer Cells Is Similar But Differentially Regulated by Histone Deacetylase Inhibitors: M. Zille, et al.; eNeuro 6, ENEURO.0263-18.2019 (2019), Abstract; Full Text
Coordination of receptor tyrosine kinase signaling and interfacial tension dynamics drive radial intercalation and tube elongation: N. Neumann, et al.; Dev. Cell 45, 67 (2018), Abstract; Full Text
Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells: N. Pizato, et al.; Sci. Rep. 8, 1952 (2018), Abstract;
Lysosomal Degradation of CD44 Mediates Ceramide Nanoliposome-induced Anoikis and Diminished Extravasation in Metastatic Carcinoma Cells: J. K. Haakenson, et al.; J. Biol. Chem. 290, 8632 (2015), Application(s): Cell Culture , Abstract; Full Text

General Literature References

Baculovirus p35 and Z-VAD-fmk inhibit thapsigargin-induced apoptosis of breast cancer cells: X.M. Qi, et al.; Oncogene 15, 1207 (1997), Abstract;
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J. 315, 21 (1996), Abstract; Full Text
Fas-induced activation of the cell death-related protease CPP32 Is inhibited by Bcl-2 and by ICE family protease inhibitors: R.C. Armstrong, et al.; J. Biol. Chem. 271, 16850 (1996), Abstract; Full Text

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