Modulation of Glucose & Lipid Metabolism

Recombinant Human Adiponectin

HMW Adiponectin Delivering Superior In Vitro Activity

Quantify Adiponectin

Adiponectin (human), ELISA Kit

A Rapid, High-sensitivity ELISA Kit for Quantification of Human Adiponectin

Adiponectin Receptor Activation

AdipoRon

A Novel Small Molecule Adiponectin Mimetic

Adipogenesis Dye Allows for Visual Confirmation of Adipocyte Differentiation



3T3-L1 cells were treated with and without induction media. After 10 days, cells were stained with Adipogenesis Dye and imaged at 40x magnification.

GLP and Exendin

GLP (glucagon-like peptide) functions in the regulation of blood glucose through insulin, and plays a role in obesity and diabetes. GLP-1 is an important gastrointestinal peptide hormone or incretin, which is released when we eat, produced by tissue specific post-translational processing from a glucagon precursor, and inactivated by dipeptidylpeptidase DPP-4 enzymes. Exendin-4 is a 39 amino-acid peptide found in venom from the Gila monster. It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. As an agonist of the GLP-1 receptor, Exendin-4 is a potent stimulator of insulin secretion and has a much longer half life than GLP-1 (~2 minutes) making it a suitable drug candidate for the treatment of obesity and type-2 diabetes.

• AMP’D® GLP-1 ELISA Kit
• GLP Antibodies
• Exendin Antibodies
• GLP Proteins
• Exendin-4 Protein

Lipocalin-2, Neutrophil Gelatinase-associated Lipocalin (NGAL)

NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues and released into both urine and blood upon kidney tubular damage. The protein is protease resistant and over-expressed during inflammation, infection and certain cancers. During acute renal failure (ARF) caused by either ischemia or nephrotoxicity, NGAL is secreted in high levels into blood and urine within 2 hours or less of injury to the kidney. NGAL is also released at the time of acute kidney injury (AKI) unlike serum creatinine, another kidney diagnostic biomarker which may not be seen until 72 hours after injury when up to 50% of renal function may have been lost.

• NGAL ELISA Kits
• NGAL Antibodies
• NGAL Proteins

Adipokines

Adipose tissue contains differentiated cells called adipocytes that are specialized in storing fat. In addition to their function in controlling energy homeostasis, adipocytes release endocrine and paracrine signaling molecules called adipokines that are important in many physiological and metabolic processes. Some adipokines such as adiponectin, omentin, Nampt (Visfatin/PBEF), resistin or RBP4 are involved in insulin signaling. Others regulate food intake and lipid metabolism (e.g. leptin, chemerin).

MITO-ID® Extracellular pH Sensor Probe

Efficiently Monitor Glycolytic Activity

• pH sensitive probe increases in signal intensity with increasing acidity
• Simple mix-and-read protocol for 96-well microplates.

MITO-ID® Extracellular O2 Sensor Kit High Sensitivity

Detect Mitochondrial Dysfunction Within Minutes of Treatment

• Phosphorescent probe increases in signal intensity with O2 consumption (diminishing O2 levels)
• Amenable to 96- and 384-well microplates for high-throughput analysis
• Multiplex with MITO-ID® pH Sensor probe to confirm mitochondrial toxicity

MITO-ID® Assays to Identify True Mitochondrial Toxicity



Multi-compound Screening using MITO-ID® Extracellular O2 Sensor Kit and MITO-ID® Extracellular pH Sensor Probe. Compound 2 shows a dose dependent effect on oxygen consumption (decrease) with an increase in acidification representing "True" mitochondrial toxicity.

MITO-ID® Extracellular O2 Sensor Probe

Reagent used in high-throughput assays to monitor mitochondrial function and respiration rates, through the kinetic measurement of extracellular oxygen consumption

MITO-ID® Intracellular O2 Sensor Probe

Sensitive fluorescence assay for direct measurement of cellular oxygen concentration

MITO-ID® Assays to Identify True Mitochondrial Toxicity



Excitation and Emission spectra of oxygen-sensitive probe MITO-ID® Extracellular O2 Sensor Probe. Left panel shows normalized excitation (EX 360-400nm; Peak 380nm). Right panel shows emission (Em 630–680nm; Peak 650nm) in oxygenated and deoxygenated conditions.

MITO-ID® Membrane Potential Detection Kit for Microscopy and Flow Cytometry

The Only Assay that Monitors Energetic Status Using a Simple Mix-and-Read, No-Wash Protocol

MITO-ID® Membrane Potential Cytotoxicity Kit for Microplates

A Real-time Mitochondrial Membrane Potential Assay with Superior Sensitivity

Detect MMP Perturbations with 10X More Sensitivity than JC-1

Glutathione (GSSG/GSH), Detection Kit

Rapid, Ultra-sensitive Kit for the Quantification of Reduced (GSH), Oxidized (GSSG), and Total Glutathione

• High sensitivity assay based on an enzymatic recycling reaction for measuring total, oxidized or reduced glutathione
• Easy-to-use, rapid kit with sample handling protocols for a wide variety of matrices
• Fast, accurate assay results in less than 30 minutes

Ultra-sensitive Detection and Analysis of Glutathione

Built upon our diverse scientific expertise and technology platforms, Enzo solutions enable you to advance your metabolic research with more than 250 ELISA kits for accurate detection of your specific analyte.