Metabolism refers to a set of chemical reactions which are responsible for transforming carbohydrates, lipids and proteins into energy. These biochemical processes are essential for maintaining homeostasis. Metabolic research focuses on better understanding basic biological mechanisms that regulate metabolism and how they are dysregulated in disorders such as obesity, diabetes, cardiovascular disease, and cancer. A common root cause in these medical conditions is an energy imbalance; better understanding of this disparity will help determine therapeutic strategies. Even though metabolism involves sequences of complex biochemical reactions, Enzo Life Sciences provides simple and novel solutions to advance your metabolic research.
Discover, Analyse and Quantify Metabolic Biomarkers
Adiponectin
Adiponectin is a protein hormone primarily produced by adipocytes which circulates at high levels (5-30 mg/L) in the blood. Adiponectin modulates a number of metabolic processes and is known to contribute to metabolic pathologies including diabetes, atherosclerosis, and obesity. It has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation.
Adiponectin receptor activation is a promising potential therapy for diabetes and other obesity-associated diseases. AdipoRon has been shown to induce insulin sensitivity, inhibit lipid synthesis and increase glucose metabolism.
Orally active making it suitable for in vivo studies
Activates adiponectin receptors: AdipoR1 and AdipoR2
Visualize Mitochondrial Dysfunction by Cellular Analysis
Mitochondria, the organelles that generate energy for the cell, have a critical role in the function of a cell. Mitochondrial disease, stemming from dysfunction, can have various effects on the body with conditions ranging from epilepsy, psychiatric illness and autism, to cardiovascular disease, liver/kidney disease, diabetes and cancer. The impact of mitochondria in so many diseases makes them ideal targets for new therapeutics.
The Only Assay that Monitors Energetic Status Using a Simple Mix-and-Read, No-Wash Protocol
The mitochondria of HeLa cells were stained with MITO-ID® Membrane Potential dye and visualized by epifluorescence microscope. Orange fluorescent aggregates are localized in the mitochondria, while green fluorescent monomers mainly stain the cytosol.
Photostable, non-toxic and selective mitochondrial dye that stains regardless of membrane potential
HeLa-TurboGreen-mitochondria cells (HeLa-mitoGFP, MarinPharm GmbH, Luckenwalde, Germany) stained with MITO-ID® Red and Hoechst 33342 (blue) dyes. MITO-ID® Red co-localizes with the EGFP-cytochrome C oxidase signal (yellow signal), demonstrating selectivity for mitochondria. Note that mitochondria in cells no longer expressing the GFP-tagged protein appear red in the composite image.
Insulin regulates the plasma glucose level by maintaining a balance between intestinal absorption, liver production, and cellular uptake of glucose and its metabolism by peripheral tissues. Insulin triggers glucose, fatty acids, and amino acids uptake into adipose tissue, muscle, and the liver. It also promotes the storage of these nutrients in the form of glycogen, lipids, and proteins. At times of starvation, low insulin levels direct insulin-sensitive cells to mobilize stored fuels.
Enzo offers hundreds of ultrasensitive, time-saving, and widely cited ELISA kits for quantifying insulin and other metabolic biomarkers.
Ultra sensitive (1.279 pg/ml) ELISA kit, enabling reduced input sample and matrix interference, for the quantification of KIM-1, an early biomarker for kidney injury or disease.