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Proinsulin ELISA kit

Ultra-sensitive Proinsulin ELISA kit enabling detection of proinsulin in human serum and plasma samples in just 3 hours.
ENZ-KIT149-0001 96 wells Inquire for pricing
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  • Ultra-sensitive measurement of proinsulin, detecting as little as 0.17 pM
  • Negligible cross reactivity with insulin
  • High throughput format with results in 3 hours for up to 40 samples in duplicate
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The Proinsulin ELISA kit is a colorimetric immunometric immunoassay kit with results in 3 hours. 
Proinsulin ELISA kit Kit box image
Proinsulin ELISA kit Standard curve
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Proinsulin ELISA kit Kit box image Proinsulin ELISA kit Standard curve

Product Details

Sensitivity:0.17 pM (1.56-50pM)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of proinsulin in human serum and plasma samples.
Wavelength:450 nm
Species reactivity:Human
Crossreactivity:Minimal cross-reactivity to similar family molecules (<1.13% for insulin).
Shipping:Blue Ice
Long Term Storage:+4°C
Contents:Microtiter plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Antibody Diluent, Conjugate, Substrate, and Stop Solution
Scientific Background:Type 2 diabetes mellitus is the most common serious metabolic condition in the world, and results from a subnormal response of tissues to insulin (insulin resistance) and a failure of the insulin-secreting beta cells to compensate. Insulin is required for glucose homeostasis and an important precursor protein to insulin is proinsulin. Proinsulin is composed of the A and B subunits of insulin and is connected by the C-peptide region. This 10.5 kDa protein, which contains 110 amino acids, is synthesized as a single chain that contains a 24 amino acid signal sequence and an 86 amino acid proinsulin propeptide. Proinsulin is processed in the endoplasmic reticulum of pancreatic β-cells and is converted within immature secretory granules into insulin and C-peptide. While mature insulin is required for glucose homeostasis and C-peptide is associated with renal function, proinsulin has low metabolic activity. However, it is biologically active and is a regulator of development in prepancreatic embryonic stages. The release of proinsulin is significantly increased in patients with type 2 diabetes as well as in individuals with impaired glucose tolerance (IGT). The levels of proinsulin can also increase with age.
UniProt ID:P01308
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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