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Ultra-sensitive measurement of proinsulin, detecting as little as 0.17 pM
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Negligible cross reactivity with insulin
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High throughput format with results in 3 hours for up to 40 samples in duplicate
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Fully quantitative results that surpass semi-quantitative Western blot analysis
The Proinsulin ELISA kit is a colorimetric immunometric immunoassay kit with results in 3 hours.
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Product Details
| Sensitivity: | 0.17 pM (1.56-50pM) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of proinsulin in human serum and plasma samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | Minimal cross-reactivity to similar family molecules (<1.13% for insulin). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Antibody Diluent, Conjugate, Substrate, and Stop Solution |
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| Scientific Background: | Type 2 diabetes mellitus is the most common serious metabolic condition in the world, and results from a subnormal response of tissues to insulin (insulin resistance) and a failure of the insulin-secreting beta cells to compensate. Insulin is required for glucose homeostasis and an important precursor protein to insulin is proinsulin. Proinsulin is composed of the A and B subunits of insulin and is connected by the C-peptide region. This 10.5 kDa protein, which contains 110 amino acids, is synthesized as a single chain that contains a 24 amino acid signal sequence and an 86 amino acid proinsulin propeptide. Proinsulin is processed in the endoplasmic reticulum of pancreatic β-cells and is converted within immature secretory granules into insulin and C-peptide. While mature insulin is required for glucose homeostasis and C-peptide is associated with renal function, proinsulin has low metabolic activity. However, it is biologically active and is a regulator of development in prepancreatic embryonic stages. The release of proinsulin is significantly increased in patients with type 2 diabetes as well as in individuals with impaired glucose tolerance (IGT). The levels of proinsulin can also increase with age. |
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| UniProt ID: | P01308 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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