JC-10 is a derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (525 nm). The principal advantages of JC-10 relative to JC-1 include improved solubility in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio, of mitochondrial membrane potential changes. Wavelength Maxima: Excitation 510nm, Emission 525nm
Product Details
| Alternative Name: | Enhanced JC-1 |
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| MW: | ~600 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Reduced mitochondria membrane potential and lysosomal acidification are associated with decreased oligomeric Aβ degradation induced by hyperglycemia: A study of mixed glia cultures: Y. C. Huang, et al.; PLoS One
17, e0260966 (2022),
Abstract;
TBC1D21 is an essential factor for sperm mitochondrial sheath assembly and male fertility: Y. Chen, et al.; Biol. Reprod.
107, 619 (2022),
Abstract;
Effects of RAD51-stimulatory compound 1 (RS-1) and its vehicle, DMSO, on pig embryo culture: C.G. Lucas, et al.; Reprod. Toxicol.
105, 44 (2021),
Abstract;
Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity: M. Kriebel, et al.; Front. Mol. Neurosci.
13, 43 (2020),
Abstract;
Full Text
An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome: U. John, et al.; Sci. Adv.
5, eaav1110 (2019),
Abstract;
Full Text
Human-Induced Pluripotent Stem Cell Model of Trastuzumab-Induced Cardiac Dysfunction in Patients With Breast Cancer: T. Kitani, et al.; Circulation
139, 2451 (2019),
Abstract;
Mitochondrial metabolic reprograming via BRAF inhibition ameliorates senescence: J.W. Kim, et al.; Exp. Gerontol.
126, 110691 (2019),
Abstract;
Degenerin channel activation causes caspase‐mediated protein degradation and mitochondrial dysfunction in adult C. elegans muscle: C.J. Gaffney, et al.; J. Cachexia Sarcopenia Muscle
7, 181 (2016),
Application(s): Flow cytometry analysis of mitochondria membrane potential in C.elegans,
Abstract;
Full Text
MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation: P. Mannam, et al.; Free Radic. Biol. Med.
101, 102 (2016),
Application(s): Detecting Mitochondrial Membrane Potential (MMP) in BMDMs,
Abstract;
PINK1 and Parkin cooperatively protect neurons against constitutively active TRP channel-induced retinal degeneration in Drosophila: Z. Huang, et al.; Cell Death Dis.
7, e2179 (2016),
Application(s): Mitochondrial membrane potential was measured by staining with 25 μM JC-10 reagent,
Abstract;
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PINK1-dependent phosphorylation of PINK1 and Parkin is essential for mitochondrial quality control: N. Zhuang, et al.; Cell Death Dis.
7, e2501 (2016),
Abstract;
Full Text
Induced peroxidase and cytoprotective enzyme expressions support adaptation of HUVECs to sustain subsequent H₂O₂ exposure: H. Patel, et al.; Microvasc. Res.
2862, 30025 (2015),
Application(s): Measument of HUVECs mitochondrial membrane polarization levels,
Abstract;
