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NAO (ultra pure)

Mitochondria dye
ENZ-52306 25 mg 204.00 USD
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10-N-Nonyl acridine orange (NAO) is an acridine orange derivative and is generally used as a fluorescent marker of the inner mitochondrial membrane in whole cells. It is believed to bind to negatively charged phospholipids. NAO accumulation in the cell seems to be related to specific interactions with mitochondrial membrane proteins and/or lipids, such as cardiolipin, and is largely independent of mitochondrial membrane potential. With respect to apoptosis, the presence of mitochondrial membrane potential can be probed with Rhodamine123 while the structure and integrity of mitochondria can be assessed using 10-N-nonyl-acridine orange. Wavelength Maxima: Excitation 495nm, Emission 519nm

Product Details

Alternative Name:Nonyl Acridine Orange, 3,6-bis(Dimethylamino)-10-nonyl-acridinium bromide
Purity:≥95% (HPLC).
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Cannabidiol induced apoptosis in human monocytes through mitochondrial permeability transition pore-mediated ROS production: H.Y. Wu, et al.; Free Radic. Biol. Med. 124, 311 (2018), Abstract;
Atractylenolide III Enhances Energy Metabolism by Increasing the SIRT-1 and PGC1α Expression with AMPK Phosphorylation in C2C12 Mouse Skeletal Muscle Cells: M.Y. Song, et al.; Biol. Pharm. Bull. 40, 339 (2017), Abstract; Full Text
Cinnamomum cassia prevents high-fat diet-induced obesity in mice through the increase of muscle energy: M.Y. Song, et al.; Am. J. Chin. Med. 45, 1017 (2017), Abstract;
Fluorescent and radiolabeled triphenylphosphonium probes for imaging mitochondria: H. Yuan, et al.; Chem. Commun. (Camb.) 49, 10361 (2013), Abstract;
Estrogen prevents oxidative damage to the mitochondria in Friedreich's ataxia skin fibroblasts: T.E. Richardson, et al.; PLoS One 7, e34600 (2012), Application(s): NAO staining measured in fibroblasts using microplate reader, Abstract; Full Text
Etoposide induces ATM-dependent mitochondrial biogenesis through AMPK activation: X. Fu, et al.; PLoS ONE 3, e2009 (2008), Abstract;
Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange: C. Ferlini & G. Scambia; Nat. Protoc. 2, 3111 (2007), Abstract;

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