Replaces Prod. #: ALX-610-018
Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. Rhodamine 123 can be used in multi-parametric analysis, without fluorescence interference, in combination with common protein labeling dyes such as cyanine-5 and AMCA. With respect to apoptosis, the presence of mitochondrial membrane potential can be probed with rhodamine 123 while the structure and integrity of mitochondria can be assessed using 10-N-nonyl-acridine orange. Wavelength Maxima: Excitation 507nm, Emission 529nm
Product Details
| Alternative Name: | 3,6-Diamino-9-(2-(methoxycarbonyl)phenyl xanthylium chloride |
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| Formula: | C21H17ClN2O3 |
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| MW: | 380.8 |
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| CAS: | 62669-70-9 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
All layers matter: Innovative three-dimensional epithelium-stroma-endothelium intestinal model for reliable permeability outcomes: M.H. Macedo, et al.; J. Control Release
341, 414 (2021),
Abstract;
Neuroprotective Effect of Cyclo-(L-Pro-L-Phe) Isolated from the Jellyfish-Derived Fungus Aspergillus flavus: D.D. Li, et al.; Mar. Drugs
19, 417 (2021),
Abstract;
PDE2 regulates membrane potential, respiration and permeability transition of rodent subsarcolemmal cardiac mitochondria: D. Liu, et al.; Mitochondrion
47, 64 (2020),
Abstract;
Full Text
Detection of de- and hyperpolarization of mitochondria of cultured astrocytes and neurons by the cationic fluorescent dye rhodamine 123: S. Kahlert, et al.; J. Neurosci. Methods
171, 87 (2008),
Abstract;
Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange: C. Ferlini & G. Scambia; Nat. Protoc.
2, 3111 (2007),
Abstract;
