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TMRE (ultra pure)

Mitochondria dye
ENZ-52309 25 mg 200.00 USD
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Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1,TMRE is widely used for measuring mitochondrial membrane potential, in addition to selectively staining mitochondria. Real-time imaging of mitochondrial membrane potential in individual cardiomyocytes within perfused rat hearts has been demonstrated with this dye, using 2-photon laser-scanning microscopy. Wavelength Maxima: Excitation 549nm, Emission 574nm

Product Details

Alternative Name:Tetramethylrhodamine ethyl ester perchlorate, 3,6-bis(Dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-xanthylium perchlorate
Purity:≥95% (HPLC)
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:RUO - Research Use Only
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Product Literature References

PUMILIO hyperactivity drives premature aging of Norad-deficient mice: F. Kopp, et al.; Elife 8, e42650 (2019), Application(s): Flow cytometry using immortalized MEFs, Abstract; Full Text
Autophagy maintains the metabolism and function of young and old (hematopoietic) stem cells: T.T. Ho, et al.; Nature 543, 205 (2017), Abstract; Full Text
The role of c-FLIP splice variants in urothelial tumours: F. Ewald, et al.; Cell Death Dis. 2, e245 (2011), Abstract; Full Text
The mitochondrial membrane potential and Ca2+ oscillations in smooth muscle: S. Chalmers & J.G. McCarron; J. Cell Sci. 121, 75 (2008), Abstract;
Real-time 2-photon imaging of mitochondrial function in perfused rat hearts subjected to ischemia/reperfusion: M. Matsumoto-Ida, et al.; Circulation 114, 1497 (2006), Abstract;

General Literature References

Quantitative measurement of mitochondrial membrane potential in cultured cells: calcium-induced de- and hyperpolarization of neuronal mitochondria: A. A. Gerencser, et. al.; J. Physiol. 590, 2845 (2012), Abstract; Full Text

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