JC-1 is widely used for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio. Wavelength Maxima: Excitation 515nm, Emission 529nm
Product Details
| Alternative Name: | 5,5’,6,6’-Tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide |
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| Formula: | C25H27Cl4IN4 |
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| MW: | 652.2 |
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| CAS: | 47729-63-5 |
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| Purity: | ≥95% (TLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Full Text
Altered mitochondrial quality control in Atg7-deficient VSMCs promotes enhanced apoptosis and is linked to unstable atherosclerotic plaque phenotype: H. Nahapetyan, et al.; Cell Death Dis.
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Application(s): mitochondrial potential measurement in transgenic mice model,
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Full Text
Inhibition of MAPKinase pathway sensitizes thyroid cancer cells to ABT-737 induced apoptosis: V. Gunda, et al.; Cancer Lett.
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Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines: V. Kuete, et al.; Springerplus
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Application(s): Analysis of mitochondrial membrane potential (MMP),
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Full Text
Oxidative stress plays major role in mediating apoptosis in filarial nematode Setaria cervi in the presence of trans-stilbene derivatives: N. Mukherjee, et al.; Free. Radic. Biol. Med.
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Application(s): Cell culture,
Abstract;
Aralar plays a significant role in maintaining the survival and mitochondrial membrane potential of BV2 microglia: C. Wang, et al.; Int. J. Physiol. Pathophysiol. Pharmacol.
7, 107 (2015),
Application(s): Flow cytometry-based JC-1 assay,
Abstract;
Full Text
ATP-induced cellular stress and mitochondrial toxicity in cells expressing purinergic P2X7 receptor: S. Seeland, et al.; Pharma. Res. Per.
3, e00123 (2015),
Application(s): Cell Culture, Fluorescence Microscopy,
Abstract;
Full Text
Circumvention of cisplatin resistance in ovarian cancer by combination of cyclosporin A and low-intensity ultrasound: T. Yu, et al.; Eur. J. Pharm. Biopharm.
91, 103 (2015),
Application(s): Flow Cytometry,
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Full Text
Retinol binding protein 4 induces mitochondrial dysfunction and vascular oxidative damage: J. Wang, et al.; Atherosclerosis
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Application(s): Cell Culture,
Abstract;
Mitochondrial membrane potential monitored by JC-1 dye: M. Reers, et al.; Methods Enzymol.
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