JC-1 is widely used for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio. Wavelength Maxima: Excitation 515nm, Emission 529nm
Product Specification
| Alternative Name: | 5,5’,6,6’-Tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide |
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| Formula: | C25H27Cl4IN4 |
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| MW: | 652.2 |
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| CAS: | 47729-63-5 |
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| Purity: | ≥95% (TLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Abstract;
Full Text
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Application(s): Analysis of mitochondrial membrane potential (MMP),
Abstract;
Full Text
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Application(s): Cell culture,
Abstract;
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7, 107 (2015),
Application(s): Flow cytometry-based JC-1 assay,
Abstract;
Full Text
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Application(s): Cell Culture, Fluorescence Microscopy,
Abstract;
Full Text
Circumvention of cisplatin resistance in ovarian cancer by combination of cyclosporin A and low-intensity ultrasound: T. Yu, et al.; Eur. J. Pharm. Biopharm.
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Application(s): Flow Cytometry,
Abstract;
Full Text
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Application(s): Cell Culture,
Abstract;
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