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As Scientists Enabling Healthcare™, we realize the value in providing relevant information to our customers working in the fields of life sciences, drug development and clinical research. We are happy to provide simple but useful tips for improving daily tasks as well as the overall quality of your research.

Chromogenic in situ Hybridization E-book

  • An Introduction to Chromogenic in situ Hybridization (CISH)
  • Top 10 Tips for Successful CISH
  • Methods to Generate Probes
  • Biotin or Digoxigenin
  • DNA vs RNA Probes
  • Sample Preparation
  • Hybridization
  • Controlling Non-specific Background with Blocking
  • Methods of Detection for CISH
  • Single vs Dual Staining
  • Common Counterstaining for CISH
  • Applications of CISH
  • FAQs on CISH

IHC E-book

  • Top 10 Tips for Successful Immunohistochemistry
  • Choosing your Fixation, Embedding, and Antigen Retrieval Method for Successful IHC
  • Controlling Unspecific Background with Blocking
  • Selecting and Optimizing Primary Antibodies for IHC
  • How to Select a Secondary Antibody
  • Various Detection Methods for IHC
  • Choosing the Right Reporter Enzyme and Chromogen
  • Multiplexing Chromogenic IHC
  • Common Counterstains for IHC
  • Useful Controls for Immunohistochemistry
  • IHC Art Gallery
  • Immunohistochemistry Troubleshooting Guide

Guide to Optimizing Protein Characterization

  • Current Food and Drug Administration Guidelines
  • How to Determine Protein Stability
  • Methods for Detecting Protein Aggregates
  • Development and Specificity of the PROTEOSTAT® Dye
  • Using PROTEOSTAT® for a Rapid Detection and Characterization of Protein Aggregates by Flow Cytometry
  • Detection of Bacterial Aggregates by Flow Cytometry
  • Using PROTEOSTAT® Reagents to Predict Aggregation
  • Propensity and Monitor Aggregation of Antibody-Drug Conjugates (ADC)


  • FISH: Fluorescent in situ Hybridization
    • What is FISH? How does it work?
    • What is the difference between FISH and ISH?
    • What samples types are compatible with FISH?
    • What information does FISH provide?
  • What are the Differences Between DNA and RNA Probes?
  • Methods for Generating FISH Probes
  • How to Use Allylamine-dUTP for FISH DNA Probe Labeling
  • The Difference Between FISH BAC Probes and FISH Oligo Probes
  • Types of FISH Probes
  • Applications of FISH in Cytogenetics
  • How to Choose the Right Cytogenetics Technique for Your Research
  • FISH Tips & Troubleshooting

Western Blot E-book

  • Western Blot: Principle and Theory
  • What are the Differences Between Northern, Southern, and Western Blotting?
  • Ten Tips for Successful Westerns
  • Five Western Blot Problems and How to Troubleshoot Them
  • How Do You Choose the Right Western Blot Detection Method?
  • Membrane Selection: A Quick Comparison of PVDF and Nitrocellulose

Immunoassay E-book

  • What are the Differences Between ELISA Assay Types?
  • 10 Tips for Successful ELISA Assays
  • In-depth Insights on ELISA:
    • %CV in ELISA: How to reduce them and why they’re important
    • Dilutional linearity, parallelism, spike-and-recovery in ELISA: How to QC your results?
    • How to validate sample dilutions and achieve linearity in new sample types

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