E-Books

As Scientists Enabling Healthcare™, we realize the value in providing relevant information to our customers working in the fields of life sciences, drug development and clinical research. We are happy to provide simple but useful tips for improving daily tasks as well as the overall quality of your research.

Chromogenic in situ Hybridization E-book

An Introduction to Chromogenic in situ Hybridization (CISH)
Top 10 Tips for Successful CISH
Methods to Generate Probes
Biotin or Digoxigenin
DNA vs RNA Probes
Sample Preparation
Hybridization
Controlling Non-specific Background with Blocking
Methods of Detection for CISH
Single vs Dual Staining
Common Counterstaining for CISH
Applications of CISH
FAQs on CISH

IHC E-book

Top 10 Tips for Successful Immunohistochemistry
Choosing your Fixation, Embedding, and Antigen Retrieval Method for Successful IHC
Controlling Unspecific Background with Blocking
Selecting and Optimizing Primary Antibodies for IHC
How to Select a Secondary Antibody
Various Detection Methods for IHC
Choosing the Right Reporter Enzyme and Chromogen
Multiplexing Chromogenic IHC
Common Counterstains for IHC
Useful Controls for Immunohistochemistry
IHC Art Gallery
Immunohistochemistry Troubleshooting Guide

Guide to Optimizing Protein Characterization

Current Food and Drug Administration Guidelines
How to Determine Protein Stability
Methods for Detecting Protein Aggregates
Development and Specificity of the PROTEOSTAT® Dye
Using PROTEOSTAT® for a Rapid Detection and Characterization of Protein Aggregates by Flow Cytometry
Detection of Bacterial Aggregates by Flow Cytometry
Using PROTEOSTAT® Reagents to Predict Aggregation
Propensity and Monitor Aggregation of Antibody-Drug Conjugates (ADC)

FISH E-Book

FISH: Fluorescent in situ Hybridization
- What is FISH? How does it work?
- What is the difference between FISH and ISH?
- What samples types are compatible with FISH?
- What information does FISH provide?
What are the Differences Between DNA and RNA Probes?
Methods for Generating FISH Probes
How to Use Allylamine-dUTP for FISH DNA Probe Labeling
The Difference Between FISH BAC Probes and FISH Oligo Probes
Types of FISH Probes
Applications of FISH in Cytogenetics
How to Choose the Right Cytogenetics Technique for Your Research
FISH Tips & Troubleshooting

Western Blot E-book

Western Blot: Principle and Theory
What are the Differences Between Northern, Southern, and Western Blotting?
Ten Tips for Successful Westerns
Five Western Blot Problems and How to Troubleshoot Them
How Do You Choose the Right Western Blot Detection Method?
Membrane Selection: A Quick Comparison of PVDF and Nitrocellulose

Immunoassay E-book

What are the Differences Between ELISA Assay Types?
10 Tips for Successful ELISA Assays
In-depth Insights on ELISA:
- %CV in ELISA: How to reduce them and why they’re important
- Dilutional linearity, parallelism, spike-and-recovery in ELISA: How to QC your results?
- How to validate sample dilutions and achieve linearity in new sample types

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