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Sensitive - accurately quantify as little as 23.4 pg/mL
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Save time - obtain results from 39 samples in duplicate in just 3 hours!
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Convenient- store all reagents at 4°C
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Pre-coated plates and ready-to-use reagents save you time
The Leptin (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.
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Product Details
| Alternative Name: | OB gene product |
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| Sensitivity: | 23.4 pg/ml (range 31.3 - 2,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human leptin in culture supernatants, plasma, and serum. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 17, TMB Substrate, Stop solution 2, Wash buffer concentrate |
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| Scientific Background: | The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic beta cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-α (inhibition). Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P41159 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Leptin favors Th17/Treg cell subsets imbalance associated with allergic asthma severity: C.M. Vollmer, et al.; Clin. Transl. Allergy
12, e12153 (2022),
Abstract;
Cross talk between serum Kisspeptin-Leptin during assisted reproduction techniques: R. Rehman, et al.; Pak. J. Med. Sci.
34, 342 (2018),
Application(s): ELISA using human serum,
Abstract;
Neck circumference is associated with non-traditional cardiovascular risk factors in individuals at low-to-moderate cardiovascular risk: cross-sectional analysis of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil): B. Almeida-Pititto, et al.; Diabetol. Metab. Syndr.
10, 82 (2018),
Abstract;
Full Text
Comparison of the effects of barnidipine+losartan compared with telmisartan+hydrochlorothiazide on several parameters of insulin sensitivity in patients with hypertension and type 2 diabetes mellitus: G. Derosa, et al.; Hypertens. Res.
38, 690 (2015),
Application(s): ELISA,
Abstract;
Telomere length in the two extremes of abnormal fetal growth and the programming effect of maternal arterial hypertension: M. Tellechea, et al.; Sci. Rep.
5, 7869 (2015),
Application(s): ELISA,
Abstract;
The Impact of LEP G-2548A and LEPR Gln223Arg Polymorphisms on Adiposity, Leptin, and Leptin-Receptor Serum Levels in a Mexican Mestizo Population: E. Chavarria-Avila, et al.; BioMed Res. Int.
2015, 539408 (2015),
Application(s): ELISA using human serum,
Abstract;
Full Text
Association between obesity-related adipokines and colorectal cancer: A case-control study and meta-analysis: R. K. Joshi, et al.; World J. Gastroenterol.
20, 7941 (2014),
Abstract;
Full Text
A disposable electrochemical immunosensor for the determination of leptin in serum and breast milk: I. Ojeda, et al.; Analyst
138, 4284 (2013),
Abstract;
Adipocytokine levels in obese and non-obese subjects: an observational study: G. Derosa, et al.; Inflammation
36, 914 (2013),
Abstract;
Serum leptin, adiponectin and endometrial cancer risk in Chinese women: Y. Ma, et al.; J. Gynecol. Oncol.
24, 336 (2013),
Abstract;
Full Text
Fermented kimchi reduces body weight and improves metabolic parameters in overweight and obese patients: E.K. Kim, et al.; Nutr. Res.
31, 436 (2011),
Application(s): EIA using human serum,
Abstract;
Plasma leptin levels and digital pulse volume in obese patients without metabolic syndrome--a pilot study: Y.H. Lin, et al.; Clin. Chim. Acta
412, 730 (2011),
Application(s): EIA using human plasma,
Abstract;
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