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25(OH) Vitamin D ELISA kit

Rapid, easy-to-use ELISA kit to quantify 25(OH) Vitamin D for bone metabolism, nutrition, endocrinology, immunity and cancer.
ADI-900-215-0001 96 wells 785.00 USD
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  • Rapid results in just 1.5 hours with up to 40 sample in duplicate or 80 samples in singlicate
  • Easy-to-use and simple protocol with rapid dissociation step to reduce errors and labor
  • Convenient and user-friendly alternative to mass spectrometry
  • Highly sensitive measurement as little as 1.98 ng/ml of 25(OH) Vitamin D
  • Correlated and comparable results to current methods
  • Controls available for use with the 25(OH) Vitamin D ELISA Kit
The 25(OH) Vitamin D ELISA kit is a complete, colorimetric, competitive immunoassay kit for the quantitative determination of 25(OH) Vitamin D in plasma and serum with results in just 1.5 hours.
25(OH) Vitamin D ELISA kit Standard curve
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25(OH) Vitamin D ELISA kit Standard curve

Product Details

Alternative Name:25-Hydroxyvitamin D
Sensitivity:1.98 ng/ml (range 0.5-1010 ng/ml)
Assay Time:~1.5 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human 25(OH) Vitamin D in plasma and serum.
Wavelength:405 nm
Species reactivity:Human
Crossreactivity:25(OH) Vitamin D3 (100%), 25(OH) Vitamin D2 (~80%), 1,25(OH)2-Vit. D2 (>100%), 1,25(OH)2-Vitamin D3 (>100%), 3-epi 25(OH) Vitamin D3 (~10%), 24,25(OH)2-Vitamin D3 (~6%) and <1%: 1α(OH) Vitamin D2, 1α(OH) Vitamin D3, Vitamin D2, Vitamin D3
Use/Stability:All kit components should be stored at -20ºC.
Shipping:Blue Ice
Long Term Storage:-20°C
Contents:Microtiter Plate, Conjugate Concentrate, Antibody, Dissociation Buffer, Conjugate Diluent, Sample Diluent, Wash Buffer Concentrate, Standards, Substrate, Stop Solution, Assay Layout
Scientific Background:Recent research efforts have shown that Vitamin D levels affect various disease states and are being linked with numerous indicators of well-being in humans. These include bone diseases such as osteoporosis and arthritis, but also additional disease including hypertension, diabetes, cancer and heart disease to name a few. Our Vitamin D ELISA kit offers an alternative to labor intensive and/or costly methods of testing for Vitamin D levels in human serum. The transformation to the active form of Vitamin D begins with 7-dehydrocholesterol being acted upon by UV rays from the sun to form parent Vitamin D3.  Alternatively, Vitamin D can be ingested as parent Vitamin D2 from various food sources, native or fortified. These parent compounds are transported to the liver and undergo hydroxylation to 25(OH)Vitamin D.  This metabolite is then transported to the kidney where it undergoes a second hydroxylation to 1,25(OH)2 Vitamin D, the biologically active form of Vitamin D. It is important to note that levels of Vitamin D metabolites increase proportionately with increased uptake of parent Vitamin D. This combined with the greater half-life and stability of 25(OH) Vitamin D in circulation versus the active form (25 days versus 8 hours) are the reasons that the detection of the 25(OH) Vitamin D metabolite is used as the indicator for total Vitamin D concentration.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021), Abstract;
Vitamin K and D Status in Patients with Knee Osteoarthritis: An Analytical Cross-sectional Study: A. Askari, et al.; Mediterr. J. Rheumatol. 32, 350 (2021), Abstract;
Compounding effect of vitamin D3 diet, supplementation, and alcohol exposure on macrophage response to mycobacterium infection: M.E. Gough, et al.; Tuberculosis (Edinb.) 116S, S42 (2019), Application(s): ELISA using culture supernatants, Abstract;
VDR gene methylation as a molecular adaption to light exposure: Historic, recent and genetic influences: E.L. Beckett, et al.; Am. J. Hum. Biol. 29, (2017), Abstract;
A study of Vitamin D Levels in hypothyroid patients, a case control study in a tertiary care hospital of central India: R. Kohokare, et al.; NJMDR 4, 89 (2016), Application(s): Vitamin D levels were measured from serum of hypothyroid patients, Full Text
Evaluation of vitamin D relationship with type 2 diabetes and systolic blood pressure: S.B. Nayak, et al.; BMJ Open Diabetes Res. Care 4, e000285 (2016), Application(s): Determination of 25(OH)D levels in human plasma and whole blood samples, Abstract; Full Text
Reduced 25-OH vitamin D in patients with autoimmune cytopenias, clinical correlations and literature review: B. Fattizzo, et al.; Autoimmun. Rev. 15, 770 (2016), Application(s): Measured Vitamin D in 40 year old humans, Abstract;
Relationship between methylation status of vitamin D-related genes, vitamin D levels, and methyl-donor biochemistry: E.L. Beckett, et al.; J. Nutr. Int. Metab. 6, 8 (2016), Application(s): ELISA using human plasma,
Vitamin D deficiency is associated with endothelial dysfunction and increases type I interferon gene expression in a murine model of systemic lupus erythematosus: J.A. Reynolds, et al.; Arthritis Rheumatol. 68, 2929 (2016), Abstract;
Vitamin D status in different stages of disease severity in dogs with chronic valvular heart disease: T. Osuga, et al.; J. Vet. Intern. Med. 29, 1518 (2015), Application(s): ELISA using dog serum, Abstract; Full Text

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