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RANKL (total), soluble (human) ELISA kit

ALX-850-019-KI01 96 wells 1,170.00 USD
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Replaces Prod. #: APO-54N-016/1

The RANKL (total), soluble (human) ELISA kit is a complete assay for the quantitative determination of free and OPG-bound human soluble RANKL levels. This kit is to be used with serum, plasma and cell culture supernatant from human origin.

Product Details

Alternative Name:ODF, OPGL, TRANCE, TNFSF 11, CD254
Sensitivity:~1.56pg/ml (range 2.2-60pmol/l)
Assay Time:Overnight + 3 hours
Application Notes:For the quantitative determination of free and OPG-bound human soluble RANKL from serum, plasma and cell culture supernatant.
Wavelength:450 nm
Species reactivity:Human
Quantity:For 96 wells (~80 tests)
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:RANKL (receptor activator of nuclear factor (NF)-κB ligand; also: osteoprotegerin ligand, OPGL), its cellular receptor, receptor activator of NF-κB (RANK), and the decoy receptor, osteoprotegerin (OPG) have been identified as the key molecular regulation system for bone remodelling. RANKL, a member of the tumor necrosis factor (TNF) family, is the main stimulatory factor for the formation of mature osteoclasts and is essential for their survival. Therefore, an increase in RANKL expression leads to bone resorption and bone loss. RANKL is produced by osteoblastic lineage cells and activated T lymphocytes. It activates its specific receptor RANK which is located on osteoclasts and dendritic cells. The effects of RANKL are counteracted by OPG which is secreted by various tissues and acts as an endogenous soluble receptor antagonist.Imbalances of the RANKL/OPG system have been related to the pathogenesis of Paget’s disease, benign and malignant bone tumors, postmenopausal osteoporosis, rheumatoid arthritis, bone metastases and hypercalcemia. It was shown in several studies that in animal models restoring of the RANKL/OPG balance (e.g. by administering OPG) reduces the severity of these disorders. It has been shown, that RANKL is produced as a membrane-bound protein on murine osteoblasts/stromal cells, and cleaved into a soluble form by a metalloprotease. Stimulators of the osteoclastogenesis such as IL-1β, IL-6, IL-11, IL-17, and TNF-α, increase the expression of RANKL and decrease OPG expression in osteoblasts/stromal cells. Cytokines inhibiting the osteoclastogenesis such as IL-13, INF-γ, and TGF-β1, suppress the expression of RANKL and stimulated OPG expression.

Molecular structure: sRANKL is a part of the TNF superfamily with high similarity to other members of that protein species. Two isoforms are produced by alternate splicing, a type II membrane protein (ISOFORM 1, 317 AA, MW 35.5 kD), and a secreted molecule (ISOFORM 2, 244 AA, MW 27.7kD), lacking the cytoplasmic and transmembrane domain. Although both forms are bioactive, the membrane bound protein seems to be the homeostatic form, while the production of soluble RANKL signals pathological conditions.
Technical Info/Product Notes:Principle: RANKL (total), soluble (human) ELISA kit is an enzyme immunoassay for the direct measurement of free and OPG-bound human soluble RANKL from biological fluids (serum, plasma and cell culture supernatant). The concentration of total soluble RANKL is indicated by an enzyme-catalysed colour conversion which is monitored by the ELISA photometer. The color intensity is directly proportional to the concentration of total soluble RANKL in the sample.
UniProt ID:O14788
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Association of genetic variants of RANK, RANKL, and OPG with ankylosing spondylitis clinical features in Taiwanese: C.M. Wang, et al.; Mediators Inflamm. 2019, 8029863 (2019), Application(s): ELISA using human serum, Abstract; Full Text
High porous titanium scaffolds showed higher compatibility than lower porous beta-tricalcium phosphate scaffolds for regulating human osteoblast and osteoclast differentiation: M. Hirota, et al.; Mater. Sci. Eng. C. Mater. Biol. Appl. 49, 623 (2015), Application(s): ELISA using human culture supernatant, Abstract;
Osteoclasts are active in bone forming metastases of prostate cancer patients: I. Roato, et al.; PLoS ONE 3, e3627 (2008), Abstract; Full Text
sRANKL and OPG in serum and synovial fluid of patients with rheumatoid arthritis in comparison to non-destructive chronic arthritis: G.E. Hein, et al.; Rheumatol. Int. 28, 765 (2008), Abstract;
An increased osteoprotegerin serum release characterizes the early onset of diabetes mellitus and may contribute to endothelial cell dysfunction: P. Secchiero, et al.; Am. J. Pathol. 169, 2236 (2006), Abstract; Full Text
Effects of oral contraceptives on circulating osteoprotegerin and soluble RANK ligand serum levels in healthy young women: L.C. Hofbauer, et al.; Clin. Endocrinol. 80, 214 (2004), Abstract;

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