Product Details
| Alternative Name: | Tumor necrosis factor-α, TNFSF 2 |
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| MW: | ~17kDa. |
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| Source: | Produced in yeast. |
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| CAS: | 94948-59-1 |
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| MI: | 14: 9812 |
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| UniProt ID: | P01375 |
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| Formulation: | Lyophilized from 0.2µm-filtered solution containing mannitol and trehalose. |
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| Purity: | ≥97% (SDS-PAGE) |
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| Endotoxin Content: | <1EU/µg protein |
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| Biological Activity: | Induces cytolysis of mouse L929 cells in the presence of 1µg/ml Actinomycin D (Prod. No. BML-GR300) (ED50≤0.05ng/ml). |
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| Specific Activity: | ≥2x107U/mg protein. Determined by standard cell cytotoxicity assay (N. Matthes & M.L. Neale; Lymphokines and Interferons, A Practical Approach; M.J. Clemens et al., eds.; IRL Press, p. 221). |
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| Reconstitution: | A quick spin is recommended followed by reconstitution in distilled, sterile water to a concentration of 0.1-1mg/ml. For further dilution, add a carrier protein to avoid loss of biological activity. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Lyophilized protein is stable at room temperature for a few weeks. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Steroid-insensitive ERK1/2 activity drives CXCL8 synthesis and neutrophilia by airway smooth muscle: S. Robins, et al.; Am. J. Respir. Cell Mol. Biol.
45, 984 (2011),
Abstract;
IL-17 Promotes p38 MAPK-Dependent Endothelial Activation Enhancing Neutrophil Recruitment to Sites of Inflammation: L. Roussel, et al.; J. Immunol.
184, 4531 (2010),
Application(s): Treatment of primary LMVECs and HUVECs for assays on endothelium activation,
Abstract;
Full Text
Expression of an Uncleavable N-terminal RasGAP Fragment in Insulin-secreting Cells Increases Their Resistance toward Apoptotic Stimuli without Affecting Their Glucose-induced Insulin Secretion: J.Y. Yang, et al.; J. Biol. Chem.
280, 32835 (2005),
Application(s): Death induction of βTC-tet cells,
Abstract;
Full Text
Presence of functional dendritic cells in patients chronically infected with hepatitis C virus: R.S. Longman, et al.; Blood
103, 1026 (2004),
Application(s): Maturation of dendritic cells,
Abstract;
Full Text
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