Replaces Prod. #: ADI-901-156
- Ultrasensitive measurement, < 1 pg/ml IL-8
- Fast results < 3 hours from up to 37 samples in duplicate
- Reproducible and fully validated in-house in a variety of sample types
- Ready-to-use reagents and pre-coated plates reduce errors and save your time
- Save with bulk kit package!
The IL-8 (human), ELISA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Ultrasensitive measurement of < 1 pg/mL IL-8 with excellent lot-to-lot reproducibility.
Figure 1. Typical Standard Curve for ADI-901-156A
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Product Details
| Alternative Name: | Interleukin-8 |
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| Sensitivity: | 0.72 pg/ml (range 7.8-1,000 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human IL-8 in culture supernatants, plasma, and serum. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Use/Stability: | Store all components at +4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter Plate, Conjugate, Antibody, Assay Buffer 13 Concentrate, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Assay Layout Sheet, Plate Sealer |
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| Scientific Background: | Interleukin-8 (IL-8) is a cytokine produced by monocytes, macrophages, neutrophils, lymphocytes, dermal fibroblasts, keratinocytes, vascular endothelial cells, melanocytes, hepatocytes and various tumor cell lines. It is also a member of the CXC family of chemokines, displaying chemotactic activity for neutrophils and T cells. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P10145 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Profiling Distinctive Inflammatory and Redox Responses to Hydrogen Sulfide in Stretched and Stimulated Lung Cells: S.G. Spassov, et al.; Antioxidants
11, 1001 (2022),
Abstract;
Responses of increasingly complex intestinal epithelium in vitro models to bacterial toll-like receptor agonists: M. Grouls, et al.; Toxicol. In Vitro
79, 105280 (2021),
Abstract;
25-OH vitamin D and interleukin-8: emerging biomarkers in cutaneous melanoma development and progression: C.D. Ene, et al.; Mediators Inflamm.
2015, 904876 (2015),
Application(s): ELISA using human serum,
Abstract;
Full Text
Prolonged and repetitive exposure to Porphyromonas gingivalis increases aggressiveness of oral cancer cells by promoting acquisition of cancer stem cell properties: N.H. Ha, et al.; Tumour Biol.
36, 9947 (2015),
Abstract;
Unsaturated fatty acids induce mesenchymal stem cells to increase secretion of angiogenic mediators: A.N. Smith, et al.; J. Cell. Physiol.
227, 3225 (2012),
Application(s): EIA using culture supernatants from human bone marrow-derived mesenchymal cells,
Abstract;
Including indigestible carbohydrates in the evening meal of healthy subjects improves glucose tolerance, lowers inflammatory markers, and increases satiety after a subsequent standardized breakfast: A. Nilsson, et al. ; J. Nutr.
138, 732 (2008),
Application(s): EIA using human serum,
Abstract;
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