For a larger pack size, please see Prod. No.
ALX-746-052.
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).
Product Details
| Alternative Name: | CpG-B |
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| Sequence: | 5’-tccatgacgttcctgacgtt-3’ (lower case letters indicate phosphorothioate linkage). |
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| MW: | 6383 (ammonium salt) |
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| Source: | Synthetic. |
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| Quantity: | 15nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate). |
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| Formulation: | Lyophilized. Sterile. |
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| Purity Detail: | Activity and endotoxin tested - TLRGRADE®. |
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| Endotoxin Content: | <0.002EU/µg (LAL test; BioWhittaker) |
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| Reconstitution: | For a 100µM stock solution, dissolve the total vial content in 150µl endotoxin-free water (included) or PBS (to order separately).To obtain optimal dissolving we recommend the following procedure:- Add 50% of the solvent and let dissolve for 10 min.- Add remaining 50% of the solvent and mix thoroughly.- Moderate warming may aid dissolving. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
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| Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For inactive control compound, please see Prod. No. ALX-746-201. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: TLR9-dependent activation of mouse macrophages by ODN 1826 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-002).
Method: Culture of 5x10
5 cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell activation was measured by IL12-p40 induction. Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No.
ALX-746-201) activates wild type mouse macrophages. Activation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).
Figure 2: TLR9-dependent mitogenic activity of ODN 1826 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-002) in mouse splenocytes.
Method: Culture of 5x10
5 cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell proliferation was analyzed after 72 hours with a 16 hours
3H-thymidine pulse.
Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No.
ALX-746-201) induces proliferation of wild type mouse splenocytes. Induction of cell proliferation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).
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Product Literature References
Distinct effects of different adjuvants in the mouse model of allergic airway inflammation: H.S. Shin, et al.; Asian Pac. J. Allergy Immunol.
40, 111 (2022),
Abstract;
Homeostatic and Pathogenic Roles of GM3 Ganglioside Molecular Species in TLR4 Signaling in Obesity: H. Kanoh, et al.; EMBO J.
39, e101732 (2020),
Abstract;
Full Text
TLR9 activation via microglial glucocorticoid receptors contributes to degeneration of midbrain dopamine neurons: L. Maatouk, et al.; Nat. Commun.
9, 2450 (2018),
Abstract;
Full Text
Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes: Y. Kameda, et al.; Biochem. Biophys. Res. Commun.
480, 215 (2016),
Application(s): Assay for production of interferon γ (IFN-γ), spleen cells,
Abstract;
Enhancing radiosensitivity of human pulmonary adenocarcinoma cell line A549 by CpG ODN1826: R. Li, et al.; Cancer Biother. Radiopharm.
26, 69 (2011),
Abstract;
The role of intermediary domain of MyD88 in cell activation and therapeutic inhibition of TLRs: M. Avbelj, et al.; J. Immunol.
187, 2394 (2011),
Abstract;
Full Text
Cutting edge: the mechanism of invariant NKT cell responses to viral danger signals: A.J. Tyznik, et al.; J. Immunol.
181, 4452 (2008),
Abstract;
Full Text
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