For a larger pack size, please see Prod. No.
ALX-746-056.
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for human and mouse TLR9 (Toll-like receptor 9).
Product Details
Sequence: | 5’-tcgtcgttttgtcgttttgtcgtt-3’(lower case letters indicate phosphorothioate linkage). |
|
MW: | 7720 (ammonium salt) |
|
Source: | Synthetic. |
|
Quantity: | 12.5nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate). |
|
Formulation: | Lyophilized. Sterile. |
|
Purity Detail: | Activity and endotoxin tested - TLRGRADE®. |
|
Endotoxin Content: | <0.002 EU/µg (LAL test; BioWhittaker) |
|
Reconstitution: | For a 100µM stock solution, dissolve the total vial content in 125µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving. |
|
Shipping: | Ambient |
|
Long Term Storage: | +4°C |
|
Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
|
Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
|
Technical Info/Product Notes: | Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For the inactive control compound, please see Prod. No. ALX-746-206. |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
T and B cell abnormalities, pneumocystis pneumonia, and chronic lymphocytic leukemia associated with an AIOLOS defect in patients: H.S. Kuehn, et al.; J. Exp. Med.
218, e20211118 (2021),
Abstract;
Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity: J.J.P. van Beek, et al.; Oncoimmunology
5, e1227902 (2016),
Application(s): Human Dendritic Cells stimulation,
Abstract;
Full Text
TLR9-signaling is required for turning retinoic acid into a potent stimulator of RP105 (CD180)-mediated proliferation and IgG synthesis in human memory B cells: A. Eriksen, et al.; Cell. Immunol.
279, 87 (2012),
Application(s): FLOW of human B cells,
Abstract;
Related Products