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cAMP complete ELISA kit

Most sensitive (0.006 pmol/ml and 0.027 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification.
 
ADI-900-163A 96 wells 482.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ADI-900-163

Alternative size available: ADI-901-163A (5x96 wells)
  • Versatile and convenient kit used to assay both intracellular and extracellular cAMP
  • Highy sensitive measurement of cAMP with an optional acetylation protocol increasing sensitivity >10-fold (0.006 pmol/ml and 0.027 pmol/ml) for intra- and extracellular cAMP, respectively
  • Simple, efficient and well-established sample handling protocols
  • Widely cited in peer-reviewed publications
  • Higher throughput format with results in 3 hours for up to 39 samples in duplicate
  • Reliable and consistent lot-to-lot performance
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.
Complete cAMP ELISA kit Standard curve - Acetylated in AB2
Typical standard curve used to calculate cAMP concentrations in Assay Buffer 2 for Acetylated assay format.
Complete cAMP ELISA kit Standard curve - Acetylated HCl
Typical standard curve used to calculate cAMP concentrations in 0.1M HCl for Acetylated assay format.
Complete cAMP ELISA kit Standard curve - Non-Acetylated in AB2
Typical standard curve used to calculate cAMP concentrations in Assay Buffer 2 for Non-acetylated assay format.
Complete cAMP ELISA kit Standard curve - Non-acetylated in HCl
Typical standard curve used to calculate cAMP concentrations in 0.1M HCl for Non-acetylated assay format.
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Complete cAMP ELISA kit Standard curve - Acetylated in AB2 Complete cAMP ELISA kit Standard curve - Acetylated HCl Complete cAMP ELISA kit Standard curve - Non-Acetylated in AB2 Complete cAMP ELISA kit Standard curve - Non-acetylated in HCl

Product Details

Alternative Name:cyclic AMP
 
Sensitivity:Non-acetylated: 0.49 pmol/ml (assay buffer) / 1.18 pmol/ml (HCl) (range (non-acetylated) 0.78 - 200 pmol/ml)
Acetlylated: 0.027 pmol/ml (assay buffer) / 0.006 pmol/ml (HCl) (range (acetylated) 0.078 - 20 pmol/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of cAMP in cell lysates, culture supernatants, serum, saliva, and tissue from any species.
 
Wavelength:405 nm
 
Species reactivity:Species independent
 
Crossreactivity:cyclic AMP (100%)
AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%)
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Contents:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Assay buffer 2, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Triethylamine, Acetic anhydride
 
Scientific Background:Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons: N.J. Yang, et al.; Nat. Neurosci. 25, 168 (2022), Abstract;
Elexacaftor is a CFTR potentiator and acts synergistically with ivacaftor during acute and chronic treatment: C.A. Shaughnessy, et al.; Sci. Rep. 11, 19810 (2021), Abstract;
Inhibition of relaxin autocrine signaling confers therapeutic vulnerability in ovarian cancer: H.E. Burston, et al.; J. Clin. Invest. 131, e142677 (2021), Abstract;
LHCGR and ALMS1 defects likely cooperate in the development of polycystic ovary syndrome indicated by double-mutant mice: L. Yu, et al.; J. Genet. Genomics 48, 384 (2021), Abstract;
β3 adrenergic receptor as potential therapeutic target in ADPKD: G. Schena, et al.; Physiol. Rep. 9, e15058 (2021), Abstract;
Coupling of melanocyte signaling and mechanics by caveolae is required for human skin pigmentation: L. Domingues, et al.; Nat. Commun. 11, 2988 (2020), Application(s): cAMP quantification in human melanocytes, Abstract; Full Text
Increased salt intake does not worsen the progression of renal cystic disease in high water-loaded PCK rats: S. Nagao, et al.; PLoS One 14, e0207461 (2019), Application(s): ELISA using rat kidney, Abstract; Full Text
Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4: I. Russo, et al.; J. Neuroinflammation 15, 297 (2018), Abstract; Full Text
Prostanoid EP2 Receptors Are Up-Regulated in Human Pulmonary Arterial Hypertension: A Key Anti-Proliferative Target for Treprostinil in Smooth Muscle Cells: J.A. Patel, et al.; Int. J. Mol. Sci. 18, 2372 (2018), Abstract; Full Text
Biventricular differences in β-adrenergic receptor signaling following burn injury: A.N. Guillory, et al.; PLoS One 12, e0189527 (2017), Abstract; Full Text
Donor variability may mask dimethyl fumarate's effects on nuclear factor E2-related factor 2 in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; BMC Res. Notes 10, 553 (2017), Abstract; Full Text
First mutation in the FSHR cytoplasmic tail identified in a non-pregnant woman with spontaneous ovarian hyperstimulation syndrome: J. Hugon-Rodin, et al.; BMC Med. Genet. 18, 44 (2017), Application(s): ELISA using COS-7 cell lysates, Abstract; Full Text
Gαs regulates Glucagon-Like Peptide 1 Receptor-mediated cyclic AMP generation at Rab5 endosomal compartment: S.B. Girada, et al.; Mol. Metab. 6, 1173 (2017), Application(s): Lysates of BRIN-BD11 pancreatic beta cells, Abstract; Full Text
Narciclasine attenuates diet-induced obesity by promoting oxidative metabolism in skeletal muscle: S.G. Julien, et al.; PLoS Biol. 15, e1002597 (2017), Abstract; Full Text
Semi-microbiological synthesis of an active lysinoalanine-bridged analog of glucagon-like-peptide-1: A. Kuipers, et al.; Peptides 91, 33 (2017), Abstract;
Supplement of cilostamide in growth medium improves oocyte maturation and developmental competence of embryos derived from small antral follicles in pigs: H. Lee, et al.; Theriogenology 91, 1 (2017), Abstract;
Astemizole-Histamine induces Beclin-1-independent autophagy by targeting p53-dependent crosstalk between autophagy and apoptosis: R. Jakhar, et al.; Cancer Lett. 372, 89 (2016), Application(s): cAMP assay using cell-conditioned media, Abstract;
Cilostamide and forskolin treatment during pre-IVM improves preimplantation development of cloned embryos by influencing meiotic progression and gap junction communication in pigs: B. Park, et al.; Theriogenology 86, 757 (2016), Application(s): Measurement of cAMP levels in oocytes, Abstract;
Dimethyl fumarate activates the prostaglandin EP2 receptor and stimulates cAMP signaling in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; Biochem. Biophys. Res. Commun. 475, 19 (2016), Application(s): PBMCs, Abstract;
Discovery of LRE1 as a specific and allosteric inhibitor of soluble adenylyl cyclase: L. Ramos-Espiritu, et al.; Nat. Chem. Biol. 12, 838 (2016), Application(s): Measurement of intracellular cAMP; Assessing reproducibility and concentration dependency, Abstract;
Modulation of breast cancer cell viability by a cannabinoid receptor 2 agonist, JWH-015, is calcium dependent: K.E. Hanlon, et al.; Breast Cancer (Dove Med. Press.) 8, 59 (2016), Application(s): Used to measure forskolin-induced cAMP formation in vitro in response to JWH-015, Abstract; Full Text
Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets: D.R. Park, et al.; PLoS One 10, e0134962 (2015), Application(s): ELISA using acetylated mouse islets, Abstract; Full Text
Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid nanoparticles in INS-1 Cells: H. Jun, et al.; J. Nanomater. 2015, Article ID 753569 (2015), Application(s): ELISA using rat INS-1 cells, Full Text
Hepatic ANGPTL3 regulates adipose tissue energy homostasis: Y. Wang, et al.; PNAS 112, 11630 (2015), Application(s): ELISA using mouse tissue homogenate, Abstract; Full Text
Misoprostol modulates cytokine expression through a cAMP pathway: Potential therapeutic implication for liver disease: L. Gobejishvili, et al.; Cell Immunol. 6616, 30041 (2015), Application(s): ELISA kits quantifying cell lysates, Abstract;
Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells: P.R. van Ginkel, et al.; Toxicol. Appl. Pharmacol. 288, 453 (2015), Application(s): ELISA using MDA-MB-231 cells, Abstract;
Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes: S.I. Kang, et al.; Biol. Pharm. Bull. 38, 552 (2015), Application(s): ELISA, Abstract; Full Text
cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, Abstract; Full Text
Dipeptidyl peptidase-4 inhibitor ameliorates early renal injury through its anti-inflammatory action in a rat model of type 1 diabetes: R. Kodera, et al.; Biochem. Biophys. Res. Commun. 443, 828 (2014), Application(s): cAMP quantification in rat urine, Abstract;
α-Tocopherol (vitamin E) stimulates cyclic AMP production in human peripheral mononuclear cells and alters immune function: S. Salinthone, et al.; Mol. Immunol. 53, 173 (2013), Application(s): Human peripheral blood mononuclear cells, Abstract;
The G Protein Coupled Receptor 3 Is Involved in cAMP and cGMP Signaling and Maintenance of Meiotic Arrest in Porcine Oocytes: C.R. Yang, et al.; PLoS One 7, e38807 (2012), Application(s): Porcine oocyte lysates, Abstract; Full Text
Lipoic acid stimulates cAMP production via G protein-coupled receptor-dependent and -independent mechanisms: S. Salinthone, et al.; J. Nutr. Biochem. 22, 681 (2011), Application(s): Human peripheral blood mononuclear cells and NK cells, Abstract; Full Text
Lipoic acid attenuates inflammation via cAMP and protein kinase A signaling: S. Salinthone, et al.; PLoS One 5, e13058 (2010), Application(s): T cell enriched PBMCs, Abstract; Full Text
A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes: D. Ríos-Cardona, et al.; Dev. Biol. 317, 380 (2008), Application(s): EIA using xenopus cell lysates, Abstract;
Lipoic acid stimulates cAMP production via the EP2 and EP4 prostanoid receptors and inhibits IFN gamma synthesis and cellular cytotoxicity in NK cells: S. Salinthone, et al.; J. Neuroimmunol. 199, 46 (2008), Application(s): NK cells, Abstract; Full Text

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011), Abstract;

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