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Safe and non-radioactive colorimetric measurement of kinase activity
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Flexible format, kinetic and end-point options available
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Fast results in < 4.5 hours
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Efficiency maximized with only 30 µl diluted sample needed per well
The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Product Details
| Alternative Name: | Protein kinase A, cAMP-dependent protein kinase |
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| Applications: | Colorimetric detection
Activity assay
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| Application Notes: | For the quantitative determination of PKA kinase activity in partially purified, purified, or crude enzyme preparations from any species. |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except active kinase at -80°C. |
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| Shipping: | Dry Ice |
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| Contents: | Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2 |
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| Scientific Background: | PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. |
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| UniProt ID: | P17612 (Cα subunit), P22694 (Cβ subunit), P22162 (Cγ subunit), P10644 (RIα subunit), P31321 (RIβ subunit), P13861 (RIIα subunit), P31323 (RIIβ subunit) |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Intranasal Administration of Forskolin and Noopept Reverses Parkinsonian Pathology in PINK1 Knockout Rats: R.K. Dagda, et al.; Int. J. Mol. Sci.
24, 690 (2023),
Abstract;
Neuroinflammation-mediated mitochondrial dysregulation involved in postoperative cognitive dysfunction: Y. Yang, et al.; Free Radic. Biol. Med.
178, 134 (2022),
Abstract;
Selective Targeting of Protein Kinase C (PKC)-θ Nuclear Translocation Reduces Mesenchymal Gene Signatures and Reinvigorates Dysfunctional CD8 + T Cells in Immunotherapy-Resistant and Metastatic Cancers: J. Dunn, et al.; Cancers
14, 1596 (2022),
Abstract;
Forskolin regulates retinal endothelial cell permeability through TLR4 actions in vitro: L. Liu, et al.; Mol. Cell. Biochem.
476, 4487 (2021),
Abstract;
Roflumilast ameliorates cognitive deficits in a mouse model of amyloidogenesis and tauopathy: Involvement of nitric oxide status, Aβ extrusion transporter ABCB1, and reversal by PKA inhibitor H89: N.H. Ashour, et al.; Prog. Neuropsychopharmacol. Biol. Psychiatry
111, 110366 (2021),
Abstract;
Role of cAMP/PKA/CREB pathway and β-arrestin 1 in LH induced luteolysis in pregnant rats: A. Vashishta, et al.; Reproduction
162, 21 (2021),
Abstract;
5-hydroxymethyl-2-furaldehyde purified from Japanese pear ( Pyrus pyrifolia Nakai cv. Nijisseiki) juice concentrate inhibits melanogenesis in B16 mouse melanoma cells: T. Bito, et al.; Biosci. Biotechnol. Biochem.
84, 2384 (2020),
Abstract;
Beta-adrenergic Agonist Protects Retinal Pigment Epithelium Against Hydroxycholoroquine Toxicity via cAMP-PKA Signal Pathway: R. Zhang, et al.; Int. J. Ophthalmol.
13, 552 (2020),
Abstract;
Full Text
Targeting Chronic Lymphocytic Leukemia With N-methylated thrombospondin-1-derived Peptides Overcomes Drug Resistance: E. Pramil, et al.; Blood Adv.
20, 2920 (2020),
Abstract;
Full Text
Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK1 cells via a cAMP/PKA-dependent pathway: S. Sunilkumar, et al.; Am. J. Physiol. Cell Physiol.
316, C913 (2019),
Abstract;
Tau inhibits PKA by nuclear proteasome-dependent PKAR2a elevation with suppresed CREB/GluA1 phosphorylation: J. Ye, at al.; Aging Cell
19, e13055 (2019),
Abstract;
Full Text
Adenosine Augmentation Evoked by an ENT1 Inhibitor Improves Memory Impairment and Neuronal Plasticity in the APP/PS1 Mouse Model of Alzheimer's Disease: C.C. Lee, et al.; Mol. Neurobiol.
55, 8936 (2018),
Abstract;
Effects of electroacupuncture on luteal regression and steroidogenesis in ovarian hyperstimulation syndrome model rat: X. Huang, et al.; Life Sci.
197, 1 (2018),
Abstract;
Effects of luteolin on regulatory proteins and enzymes for myocyte calcium circulation in hypothermic preserved rat heart: Q. Yan, et al.; Exp. Ther. Med.
15, 1433 (2018),
Abstract;
Full Text
Impaired Ca2+ cycling of nonischemic myocytes contributes to sarcomere dysfunction early after myocardial infarction: A. Kronenbitter, et al.; J. Mol. Cell. Cardiol.
119, 28 (2018),
Abstract;
Proinflammatory switch from Gαs to Gαi signaling by Glucagon-like peptide-1 receptor in murine splenic monocyte following burn injury: Q.M. Zhang, et al.; Inflamm. Res.
67, 157 (2018),
Abstract;
Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner: R.M. Khedr, et al.; Life Sci.
211, 31 (2018),
Abstract;
The BACE1 product sAPPβ induces ER stress and inflammation and impairs insulin signaling: G. Botteri, et al.; Metabolism
85, 59 (2018),
Abstract;
The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A: D. Aquado-Liera, et al.; Neuroscience
374, 104 (2018),
Abstract;
β-Adrenergic Receptor Trafficking, Degradation, and Cell Surface Expression Are Altered in Dermal Fibroblasts from Hypertrophic Scars: A. El Ayadi, et al.; J. Invest. Dermatol.
138, 1645 (2018),
Abstract;
Adenylyl cyclase 3/adenylyl cyclase-associated protein 1 (CAP1) complex mediates the anti-migratory effect of forskolin in pancreatic cancer cells: S.N. Quinn, et al.; Mol. Carcinog.
56, 1344 (2017),
Abstract;
Biventricular differences in β-adrenergic receptor signaling following burn injury: A.N. Guillory, et al.; PLoS One
12, e0189527 (2017),
Abstract;
Full Text
Fasting regulates EGR1 and protects from glucose- and dexamethasone-dependent sensitization to chemotherapy: S. Di Biase, et al.; PLoS Biol.
15, e2001951 (2017),
Abstract;
Full Text
Fasting-Mimicking Diet Promotes Ngn3-Driven β-Cell Regeneration to Reverse Diabetes: C.W. Cheng, et al.; Cell
168, 775 (2017),
Abstract;
Full Text
Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression: X. Li, et al.; Theranostics
7, 4671 (2017),
Application(s): Medium supernatant of Human umbilical vein endothelial cells (HUVECs),
Abstract;
Full Text
Intermittent Administration of Parathyroid Hormone 1-34 Enhances Osteogenesis of Human Mesenchymal Stem Cells by Regulating Protein Kinase Cδ: S.W. Kuo, et al.; Int. J. Mol. Sci.
18, 2221 (2017),
Abstract;
Full Text
Pancreatic-derived factor impaired glucagon-like Peptide-1 production from GLUTag enterendorine L-cell line and intestines: F. Lai, et al.; Mol. Cell. Endocrinol.
452, 110 (2017),
Application(s): GLUTag L-cell lysates and primary ileum lysates,
Abstract;
PINK1 regulates mitochondrial trafficking in dendrites of cortical neurons through mitochondrial PKA: T. Das Banerjee, et al.; J. Neurochem.
142, 545 (2017),
Abstract;
Full Text
Protein kinase A determines platelet life span and survival by regulating apoptosis: L. Zhao, et al.; J. Clin. Invest.
127, 4338 (2017),
Abstract;
Full Text
Tead1 is required for maintaining adult cardiomyocyte function, and its loss results in lethal dilated cardiomyopathy: R. Liu, et al.; JCI Insight
2, 93343 (2017),
Abstract;
Full Text
Afzelin positively regulates melanogenesis through the p38 MAPK pathway: E. Jung, et al. ; Chem. Biol. Interact.
254, 167 (2016),
Application(s): PKA kinase activity was measured in cell lysates,
Abstract;
An analogue of atrial natriuretic peptide (C-ANP4-23) modulates glucose metabolism in human differentiated adipocytes: F.J. Ruiz-Ojeda, et al.; Mol. Cell. Endocrinol.
431, 101 (2016),
Abstract;
Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes: F.J. Ruiz-Ojeda et al.; PLoS One
11, e0152550 (2016),
Abstract;
Full Text
Linagliptin Has Wide-Ranging Anti-Inflammatory Points of Action in Human Umbilical Vein Endothelial Cells: Y. Nakamura, et al.; Jpn. Clin. Med.
7, 27 (2016),
Abstract;
Full Text
Protein Kinase A Activation Promotes Cancer Cell Resistance to Glucose Starvation and Anoikis: R. Palorini, et al.; PLoS One
12, e1005931 (2016),
Application(s): Determination of PKA activity,
Abstract;
Vasopressin/V2 receptor stimulates renin synthesis in the collecting duct: A. Gonzalez, et al.; Am. J. Physiol. Renal Physiol.
310, F284 (2016),
Abstract;
Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway: F. Ando, et al.; Nat. Commun.
7, 13636 (2016),
Abstract;
Full Text
Activation of OR1A1 suppresses PPAR-γ expression by inducing HES-1 in cultured hepatocytes: C. Wu, et al.; Int. J. Biochem. Cell. Biol.
64, 75 (2015),
Application(s): ELISA using human cells,
Abstract;
Activation of PKA/CREB Signaling is Involved in BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells: H. Zhang, et al.; Cell. Physiol. Biochem.
37, 548 (2015),
Application(s): ELISA measuring PKA in mouse samples,
Abstract;
Full Text
FNDC5 overexpression and irisin ameliorate glucose/lipid metabolic derangements and enhance lipolysis in obesity: X.Q. Xiong, et al.; Biochim. Biophys. Acta.
1852, 1867 (2015),
Application(s): ELISA using mouse,
Abstract;
Human monocyte recognition of adenosine-based cyclic dinucleotides unveils the A2a Gαs protein-coupled receptor tonic inhibition of mitochondrially induced cell death: M. Tosolini, et al.; Mol. Biol. Cell.
35, 479 (2015),
Application(s): PKA kinase activity in purified human monocytes,
Abstract;
Full Text
In situ fabrication of cleavable peptide arrays on polydimethylsiloxane and applications for kinase activity assays: H.H. Chen, et al.; Anal. Chim. Acta
865, 53 (2015),
Application(s): Assay,
Abstract;
Phosphodiesterase-4D Knock-down in the Prefrontal Cortex Alleviates Chronic Unpredictable Stress-Induced Depressive-Like Behaviors and Memory Deficits in Mice: Z.Z. Wang, et al.; Sci. Rep.
5, 11332 (2015),
Application(s): ELISA using mouse sample,
Abstract;
Full Text
PreImplantation Factor bolsters neuroprotection via modulating Protein Kinase A and Protein Kinase C signaling: M. Mueller, et al.; Cell Death Differ.
22, 2078 (2015),
Application(s): ELISA using mouse neuroblastoma cells,
Abstract;
Full Text
Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes: K. Ikoma-Seki, et al.; PLOS one
10, e0141378 (2015),
Abstract;
Full Text
Short- and long-term regulation of intestinal Na+/H+ exchange by Toll-like receptors TLR4 and TLR5: J.M. Cabral, et al.; Am. J. Physiol. Gastrointest. Liver Physiol.
309, G703 (2015),
Abstract;
STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs: G. Garcia-Alvarez, et al.; Mol. Biol. Cell.
26, 1141 (2015),
Application(s): PKA kinase activity in primary rat hippocampal neurons,
Abstract;
Full Text
Nicotine shifts the temporal activation of hippocampal protein kinase A and extracellular signal-regulated kinase 1/2 to enhance long-term, but not short-term, hippocampus-dependent memory: T. Gould, et al.; Neurobiol. Learn. Mem.
109, 151 (2014),
Application(s): EIA using mouse hippocampal tissue,
Abstract;
Full Text
Nitrite activates protein kinase A in normoxia to mediate mitochondrial fusion and tolerance to ischaemia/reperfusion: C. Kamga, et al.; Cardiovasc. Res.
101, 57 (2014),
Application(s): Assay of rat cardiomyocytes,
Abstract;
Full Text
Adenylyl cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A pathway activation: M. Sabbatini, et al.; J. Physiol.
591, 3693 (2013),
Application(s): EIA using mouse pancreatic tissue ,
Abstract;
Full Text
HPRT-deficiency dysregulates cAMP-PKA signaling and phosphodiesterase 10A expression: mechanistic insight and potential target for Lesch-Nyhan disease?: G.H. Guibinga, et al.; PLoS One
8, e63333 (2013),
Abstract;
Full Text
Protein kinase A-Iα regulates Na,K-ATPase endocytosis in alveolar epithelial cells exposed to high CO(2) concentrations: E. Lecuona, et al.; Am. J. Respir. Cell Mol. Biol.
48, 626 (2013),
Abstract;
Short-and Long-Term Regulation of Intestinal Na+/H+ Exchange Activity Associated with TLR2 Receptor Activation Is Independent of Nuclear Factor-κB Signaling: J. Cabral, et al.; J. Pharmacol. Exp. Ther.
346, 453 (2013),
Application(s): Assay of human T84 cells, mouse lung epithelial cells, and rat intestinal epithelial cells,
Abstract;
Full Text
The protein kinase A regulatory subunit R1A (Prkar1a) plays critical roles in peripheral nerve development: L. Guo, et al.; J. Neurosci.
33, 17967 (2013),
Application(s): Analysis of mouse sciatic nerve cells and tissue by assay, WB,
Abstract;
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Up-regulation of ciliary neurotrophic factor in astrocytes by aspirin: implications for remyelination in multiple sclerosis: K.K. Modi, et al.; J. Biol. Chem.
288, 18533 (2013),
Abstract;
Alzheimer's β-secretase (BACE1) regulates the cAMP/PKA/CREB pathway independently of β-amyloid: Y. Chen, et al.; J. Neurosci.
32, 11390 (2012),
Abstract;
Full Text
Efficacy of concurrent administration of cilostazol and methotrexate in rheumatoid arthritis: pharmacologic and clinical significance: H.Y. Kim, et al.; Life Sci.
91, 250 (2012),
Abstract;
Mechanisms underlying the inhibitory effects of uroguanylin on NHE3 transport activity in renal proximal tubule: L.M. Lessa, et al.; Am. J. Physiol. Renal Physiol.
303, F1399 (2012),
Abstract;
Selective β2-adrenoreceptor stimulation attenuates myocardial cell death and preserves cardiac function after ischemia-reperfusion injury: S. Bhushan, et al.; Arterioscler. Thromb. Vasc. Biol.
32, 1865 (2012),
Abstract;
Full Text
Limonene, a natural cyclic terpene, is an agonistic ligand for adenosine A(2A) receptors: H.M. Park, et al.; BBRC
404, 345 (2011),
Abstract;
Neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via cAMP/PKA/CREB pathway in PC12 cells: H.C. Lai, et al.; PLoS One
6, e28280 (2011),
Abstract;
Full Text
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