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Non-radioactive measurement of kinase activity
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Flexible format, kinetic and end-point options available
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Fast results in < 4.5 hours
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Efficiency maximized with only 30 µl diluted sample needed per well
The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Product Details
| Alternative Name: | Protein kinase C |
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| Assay Time: | 4.5 hours |
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| Applications: | Colorimetric detection
Activity assay
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| Application Notes: | For the measurement of PKC kinase activity partially purified, purified, or crude enzyme preparations from any species. Cited sample type includes tissue (ref. 1). |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except active kinase at -80°C. |
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| Shipping: | Dry Ice |
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| Contents: | Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Regulatory Mechanism on Anti-Glycolytic and Anti-Metastatic Activities Induced by Strobilanthes crispus in Breast Cancer, In Vitro: S.N.H. Muhammad, et al.; Pharmaceuticals (Basel)
16, 153 (2023),
Abstract;
Acyl-CoA: diacylglycerol acyltransferase 1 inhibition in the small intestine increases plasma transaminase activity via the activation of protein kinase C pathway: H. Yokoyama, et al.; J. Toxicol. Sci.
19, 47 (2022),
Abstract;
Decoy receptor 3 is involved in epidermal keratinocyte commitment to terminal differentiation via EGFR and PKC activation: N.L. Wu, et al.; Exp. Mol. Med.
54, 542 (2022),
Abstract;
Epigallocatechin-3-Gallate Allosterically Activates Protein Kinase C-α and Improves the Cognition of Estrogen Deficiency Mice: S. Zhang, et al.; ACS Chem. Neurosci.
12, 3672 (2021),
Abstract;
Nobiletin Promotes Megakaryocytic Differentiation through the MAPK/ERK-Dependent EGR1 Expression and Exerts Anti-Leukemic Effects in Human Chronic Myeloid Leukemia (CML) K562 Cells: J.H. Yen, et al.; Cells
9, 877 (2020),
Abstract;
Full Text
CXCL12γ Promotes Metastatic Castration-Resistant Prostate Cancer by Inducing Cancer Stem Cell and Neuroendocrine Phenotypes: Y. Jung, et al.; Cancer Res.
78, 2026 (2018),
Abstract;
Discovery of a small-molecule protein kinase Cδ-selective activator with promising application in colon cancer therapy: C. Bessa, et al.; Cell Death Dis.
9, 23 (2018),
Abstract;
Full Text
Protein Kinase C Activity and Delayed Recovery of Sleep-Wake Cycle in Mouse Model of Bipolar Disorder: E. Moon, et al.; Psychiatry Investig.
15, 907 (2018),
Abstract;
Protein kinase C-dependent cell damage by unsaturated carbonyl compounds in vascular cells: T. Higashi, et al.; J. Biosci. Bioeng.
126, 527 (2018),
Abstract;
Proteomics Reveals the Molecular Underpinnings of Stronger Learning and Memory in Eastern Compared to Western Bees: L. Meng, et al.; Mol. Cell. Proteomics
17, 255 (2018),
Abstract;
Full Text
Saxagliptin but Not Sitagliptin Inhibits CaMKII and PKC via DPP9 Inhibition in Cardiomyocytes: C.N. Koyani, et al.; Front. Physiol.
9, 1622 (2018),
Abstract;
Full Text
Transient activation of PKC results in long-lasting detrimental effects on systolic [Ca2+]i in cardiomyocytes by altering actin cytoskeletal dynamics and T-tubule integrity: A. Guo, et al.; J. Mol. Cell. Cardiol.
115, 104 (2018),
Abstract;
Intermittent Administration of Parathyroid Hormone 1-34 Enhances Osteogenesis of Human Mesenchymal Stem Cells by Regulating Protein Kinase Cδ: S.W. Kuo, et al.; Int. J. Mol. Sci.
18, 2221 (2017),
Abstract;
Full Text
Lead-Induced ERK Activation Is Mediated by GluR2 Non-containing AMPA Receptor in Cortical Neurons: K. Ishida, et al.; Biol. Pharm. Bull.
40, 303 (2017),
Abstract;
Full Text
Astemizole-Histamine induces Beclin-1-independent autophagy by targeting p53-dependent crosstalk between autophagy and apoptosis: R. Jakhar, et al.; Cancer Lett.
372, 89 (2016),
Application(s): Protein kinase C assay,
Abstract;
Ceramide inhibits PKCθ by regulating its phosphorylation and translocation to lipid rafts in Jurkat cells: R. Hage-Sleiman, et al.; Immunol. Res.
64, 69 (2016),
Abstract;
DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis: E. Lee, et al.; Neoplasia
18, 553 (2016),
Abstract;
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Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs: N. Sahu, et al.; Nat. Commun.
7, 12351 (2016),
Abstract;
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Intracellular regulation of the insect chemoreceptor complex impacts odour localization in flying insects: M.N. Getahun, et al.; J. Exp. Biol.
219, 3428 (2016),
Abstract;
Involvement of acetylcholinesterase and protein kinase c in the protective effect of caffeine against β-amyloid-induced alterations in red blood cells: C. Carelli-Alinovi, et al.; Biochimie
121, 52 (2016),
Application(s): Determine PKC activity in RBCs extracts,
Abstract;
Linagliptin Has Wide-Ranging Anti-Inflammatory Points of Action in Human Umbilical Vein Endothelial Cells: Y. Nakamura, et al.; Jpn. Clin. Med.
7, 27 (2016),
Abstract;
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Nuclear PKC-θ facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation: J. Li, et al.; J. Cell Sci.
129, 2448 (2016),
Abstract;
Full Text
Protein kinase C-mediated sodium glucose transporter 1 activation in precondition-induced cardioprotection: A. Kanwal, et al.; Drug Des. Devel. Ther.
10, 2929 (2016),
Application(s): PKC activity assay, heart homogenate,
Abstract;
Full Text
Wedelolactone mitigates UVB induced oxidative stress, inflammation and early tumor promotion events in murine skin: plausible role of NFkB Pathway: F. Ali, et al.; Eur. J. Pharmacol.
786, 253 (2016),
Application(s): PKC activity was evaluated,
Abstract;
Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact.
237, 104 (2015),
Application(s): Assay using rat aortic tissue homogenate ,
Abstract;
NADPH oxidase NOX1 is involved in activation of protein kinase C and premature senescence in early-stage diabetic kidney: K. Zhu, et al.; Free Radic. Biol. Med.
83, 21 (2015),
Application(s): Assay,
Abstract;
PKC-α contributes to high NaCl-induced activation of NFAT5 (TonEBP/OREBP) through MAPK ERK1/2: H. Wang, et al.; Am. J. Physiol. Renal Physiol.
308, F140 (2015),
Abstract;
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PKC-α-dependent augmentation of cAMP and CREB phosphorylation mediates the angiotensin II stimulation of renin in the collecting duct: A.A. Gonzalez, et al.; Am. J. Physiol. Renal Physiol.
309, F880 (2015),
Abstract;
Full Text
Porphyromonas gingivalis-Induced GEF Dock180 Activation by Src/PKCδ-Dependent Phosphorylation Mediates PLCγ2 Amplification in Salivary Gland Acinar Cells: Modulatory Effect of Ghrelin: B.L. Slomiany, et al.; J. Biosci. Med.
3, 66 (2015),
Application(s): Assay using rat acinar cells of sublingual salivary gland,
Full Text
PreImplantation Factor bolsters neuroprotection via modulating Protein Kinase A and Protein Kinase C signaling: M. Mueller, et al.; Cell Death Differ.
22, 2078 (2015),
Application(s): ELISA using mouse neuroblastoma cells,
Abstract;
Full Text
Short- and long-term regulation of intestinal Na+/H+ exchange by Toll-like receptors TLR4 and TLR5: J.M. Cabral, et al.; Am. J. Physiol. Gastrointest. Liver Physiol.
309, G703 (2015),
Abstract;
Simvastatin Impairs Insulin Secretion by Multiple Mechanisms in MIN6 Cells: N. Yaluri, et al.; PLoS One
10, e0142902 (2015),
Abstract;
Full Text
Urea-induced ROS cause endothelial dysfunction in chronic renal failure: M. D'Apolito, et al.; Atherosclerosis
239, 393 (2015),
Application(s): Assay,
Abstract;
CCK8 negatively regulates the TLR9-induced activation of human peripheral blood pDCs by targeting TRAF6 signaling: X. Jia, et al.; Eur. J. Immunol.
44, 489 (2014),
Abstract;
Kainate receptor activation induces glycine receptor endocytosis through PKC deSUMOylation: H. Sun, et al.; Nat. Commun.
5, 4980 (2014),
Abstract;
Full Text
Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis: E.C. Keats, et al.; Stem Cells
32, 1649 (2014),
Abstract;
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Chromatinized protein kinase C-theta: can it escape the clutches of NF-kappaB?: E.L. Sutcliffe, et al.; Front. Immun.
3, 260 (2012),
Abstract;
Chromatinized Protein Kinase C-θ: Can It Escape the Clutches of NF-κB?: E.L. Sutcliffe, et al.; Front. Immunol.
3, 260 (2012),
Abstract;
Full Text
Cutting edge: Slamf8 is a negative regulator of Nox2 activity in macrophages: G. Wang, et al.; J. Immunol.
188, 5829 (2012),
Abstract;
Full Text
PKC-Dependent Human Monocyte Adhesion Requires AMPK and Syk Activation: M. Chang, et al.; PLoS One
7, e40999 (2012),
Abstract;
Reduced Activity of Protein Kinase C in the Frontal Cortex of Subjects with Regressive Autism: Relationship with Developmenal Abnormalities: J. Lina, et al.; Int. J. Biol. Sci.
8, 1075 (2012),
Abstract;
Anti-inflammatory actiity of Chios mastic gum is associated with inhibition of TNF-alpha induced oxidative stress: A. Triantafyllou, et al.; Nutrition
10, 64 (2011),
Abstract;
Cigarette smoke extract upregulates heme oxygenase-1 via PKC/NADPH oxidase/ROS/PDGFR/PI3K/Akt pathway in mouse brain endothelial cells: R.H. Shih, et al.; J. Neuroinflammation
8, 104 (2011),
Abstract;
Full Text
Anti-inflammatory and antitumor promotional effects of a novel urinary metabolite, 3',4'-didemethylnobiletin, derived from nobiletin: M. Pan, et al. ; Carcinogenesis
29, 2415 (2008), Application(s): EIA using mouse tissue (ref. 1),
Abstract;
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