Replaces Prod. #: ADI-901-067
- Trusted – regularly cited in peer-reviewed publications
- Sensitive – optional acetylation protocol increases sensitivity >10-fold (0.027 pmol/ml)
- Rapid – results in just 3 hours from up to 39 samples in duplicate
- Easy-to-use – pre-coated plate and liquid color-coded reagents reduce errors
- Simple – efficient and well-established sample handling protocols
- Reliable – consistent lot-to-lot performance
- Save with bulk kit package!
This is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of extracellular cAMP. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.
Typical standard curve used to calculate cAMP concentrations for Acetylated assay format.
Typical standard curve used to calculate cAMP concentrations for Non-Acetylated assay format.
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Product Details
| Alternative Name: | cyclic AMP |
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| Sensitivity: | Non-acetylated: 0.49 pmol/ml (range 0.78 - 200 pmol/ml)
Acetylated: 0.027 pmol/ml (range 0.078 - 20 pmol/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of cAMP in culture supernatants, serum, and saliva from any species. Cited sample types include cell lysate, tissue and urine. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Crossreactivity: | cyclic AMP (100%)
AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%) |
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| Use/Stability: | Store all components at 4°C, except standard and conjugate at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | GxR Microtiter plate, Conjugate, Antibody, Assay buffer 2 concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine |
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| Scientific Background: | Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
A Series of Substituted Bis-Aminotriazines Are Activators of the Natriuretic Peptide Receptor C: R.J. Smith, et al.; J. Med. Chem.
65, 5495 (2022),
Abstract;
Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons: N.J. Yang, et al.; Nat. Neurosci.
25, 168 (2022),
Abstract;
Evaluation of Multivalent Enterotoxigenic Escherichia coli Vaccine Candidate MecVax Antigen Dose-Dependent Effect in a Murine Model: H. Seo, et al.; Appl. Environ. Microbiol.
88, e0095922 (2022),
Abstract;
Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization: C. Anastasaki, et al.; STAR Protocols
3, 101173 (2022),
Abstract;
Involvement of BMP-15 in glucocorticoid actions on ovarian steroidogenesis by rat granulosa cells: C. Kashino, et al.; Biochem. Biophys. Res. Commun.
559, 56 (2021),
Abstract;
Short-term overnutrition induces white adipose tissue insulin resistance through sn-1,2-diacylglycerol - PKCε - insulin receptorT1160 phosphorylation: K. Lyu, et al.; JCI Insight
2021, 139946 (2021),
Abstract;
β3 adrenergic receptor as potential therapeutic target in ADPKD: G. Schena, et al.; Physiol. Rep.
9, e15058 (2021),
Abstract;
Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death: F. Velard, et al.; Sci. Rep.
10, 16740 (2020),
Abstract;
Full Text
Adenosine-Functionalized Biodegradable PLA-b-PEG Nanoparticles Ameliorate Osteoarthritis in Rats: X. Liu, et al.; Sci. Rep.
9, 7430 (2019),
Abstract;
Full Text
Tyrosine kinase inhibitors relax pulmonary arteries in human and murine precision-cut lung slices: A.D. Rieg, et al.; Respir. Res.
20, 111 (2019),
Application(s): Lysates of human pulmonary arteries and veins,
Abstract;
Full Text
Endothelin-1 promotes human germinal vesicle-stage oocyte maturation by downregulating connexin-26 expression in cumulus cells: L. Cui, et al.; Mol. Hum. Reprod.
24, 27 (2018),
Application(s): Medium supernatant from cumulus cells,
Abstract;
Genetic immunization with mouse thyrotrophin hormone receptor plasmid breaks self-tolerance for a murine model of autoimmune thyroid disease and Graves' orbitopathy: A. Schlüter, et al.; Clin. Exp. Immunol.
191, 255 (2018),
Application(s): ELISA - CHO cell supernatant,
Abstract;
Full Text
Incretins modulate progesterone biosynthesis by regulating bone morphogenetic protein activity in rat granulosa cells: Y. Nishiyama, et al.; J. Steroid Biochem. Mol. Biol.
178, 82 (2018),
Application(s): Medium supernatant from rat granulosa cells,
Abstract;
Interaction between orexin A and bone morphogenetic protein system on progesterone biosynthesis by rat granulosa cells: S. Fujita, et al.; J. Steroid Biochem. Mol. Biol.
181, 73 (2018),
Application(s): Medium supernatant from rat granulosa cells,
Abstract;
A regulatory role of androgen in ovarian steroidogenesis by rat granulosa cells: T. Hasegawa, et al.; J. Steroid Biochem. Mol. Biol.
172, 160 (2017),
Application(s): Medium supernatant from rat granulosa cells,
Abstract;
Exendin-4 increases oxygen consumption and thermogenic gene expression in muscle cells: J.S. Choung, et al.; J. Mol. Endocrinol.
58, 79 (2017),
Abstract;
LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse: V. Rossi, et al.; Cell Death Differ.
24, 72 (2017),
Application(s): ELISA using lysate of primary ovarian tissue culture,
Abstract;
Full Text
Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis: H. Müller-Fielitz, et al.; Nat. Commun.
8, 484 (2017),
Application(s): ELISA - CHO cell lysate,
Abstract;
Full Text
Angelica dahurica Extracts Improve Glucose Tolerance through the Activation of GPR119: E.Y. Park, et al.; PLoS One
11, e0158796 (2016),
Application(s): cAMP assay, mouse cells,
Abstract;
Full Text
cAMP regulates the functional activity, coupling efficiency and structural organization of mammalian FOF1ATP synthase: D. De Rasmo, et al.; Biochim. Biophys. Acta
1857, 350 (2016),
Application(s): cAMP level was measured in rat liver mitochondria samples,
Abstract;
cAMP/PKA/CREB/GLT1 signaling involved in the antidepressant-like effects of phosphodiesterase 4D inhibitor (GEBR-7b) in rats: X. Liu, et al.; Neuropsychiatr. Dis. Treat.
12, 219 (2016),
Application(s): cAMP levels were measured in rat samples,
Abstract;
Full Text
Cyclic AMP Affects Oocyte Maturation and Embryo Development in Prepubertal and Adult Cattle: S.M. Bernal-Ulloa, et al.; PLoS One
11, e0150264 (2016),
Application(s): Bovine oocyte lysates,
Abstract;
Full Text
Dimethyl fumarate activates the prostaglandin EP2 receptor and stimulates cAMP signaling in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; Biochem. Biophys. Res. Commun.
475, 19 (2016),
Application(s): cAMP levels were measured,
Abstract;
Effects of Human Mesenchymal Stem Cells Transduced with Superoxide Dismutase on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Mice: S.K. Sah, et al.; Antioxid. Redox Signal.
24, 233 (2016),
Application(s): Mouse tissue lysate and blood plasma,
Abstract;
Full Text
Nucleotides protect rat brain astrocytes against hydrogen peroxide toxicity and induce antioxidant defense via P2Y receptors: D. Förster & G. Reise; Neurochem. Int.
94, 57 (2016),
Application(s): Measurement of cAMP levels in astrocytes,
Abstract;
p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway: M. Ma, et al.; Sci. Rep.
6, 31315 (2016),
Application(s): cAMP content in mouse cells and serum,
Abstract;
Full Text
Activation of PKA/CREB Signaling is Involved in BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells: H. Zhang, et al.; Cell. Physiol. Biochem.
37, 548 (2015),
Application(s): ELISA measuring PKA in mouse samples,
Abstract;
Full Text
Antagonizing Effects of Aspartic Acid against Ultraviolet A-Induced Downregulation of the Stemness of Human Adipose Tissue-Derived Mesenchymal Stem Cells: K. Jung, et al.; PLoS One
10, e0124417 (2015),
Application(s): ELISA using human culture supernatant ,
Abstract;
Full Text
Intrathecal Delivery of IL-6 Reactivates the Intrinsic Growth Capacity of Pyramidal Cells in the Sensorimotor Cortex after Spinal Cord Injury: P. Yang, et al.; PLoS One
10, e0127772 (2015),
Abstract;
Full Text
Leukotriene D4 Requires PKCα- Akt Signaling Pathway to Inhibit Dependent Alanine Cotransporter (ASCT1) in Enterocytes: J.R. Talukder, et al.; J. Gastrointest. Dig. Syst.
5, 6 (2015),
Application(s): Determination of intracellular cAMP,
Full Text
Luciferase Reporter Gene Assay on Human 5-HT Receptor: Which Response Element Should Be Chosen: Y. Chen, et al.; Sci. Rep.
5, 8060 (2015),
Application(s): ELISA,
Abstract;
Quercetin Inhibits Pacemaker Potentials via Nitric Oxide/cGMP-Dependent Activation and TRPM7/ANO1 Channels in Cultured Interstitial Cells of Cajal from Mouse Small Intestine: H. Gim, et al.; Cell. Physiol. Biochem.
35, 2422 (2015),
Application(s): ELISA using murine small intestine interstitial cells of Cajal (ICCs),
Abstract;
Full Text
Thyrostimulin Regulates Osteoblastic Bone Formation During Early Skeletal Development: J.H. Duncan Bassett, et al.; Endocrinology
156, 3098 (2015),
Application(s): ELISA using CHO cell lysate,
Abstract;
Full Text
Characterization of Thyrotropin Receptor Antibody-Induced Signaling Cascades: S.A. Morshed, et al. ; Endocrinology
150, 519 (2009),
Application(s): EIA using hamster & rat cell lysates,
Abstract;
Locomotor activity, ultrasonic vocalization and oxytocin levels in infant CD38 knockout mice: H. Higashida, et al. ; Neurosci. Lett.
448, 67 (2008), Application(s): EIA using mouse culture supernatants & tissue (ref. 2),
Abstract;
Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: K.K. Parker, et al. ; Neurochem. Int.
50, 109 (2007),
Application(s): EIA using hamster cell lysates,
Abstract;
The phosphodiesterase 5 inhibitor sildenafil stimulates angiogenesis through a protein kinase G/MAPK pathway: A. Papapetropoulos, et al. ; J. Cell. Physiol.
211, 197 (2007),
Application(s): EIA using human cell lysates,
Abstract;
High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol.
284, H1124 (2003),
Application(s): EIA using rat cell lysates,
Abstract;
Phosphoinositide 3-Kinase Activity Is Required for Biphasic Stimulation of Cyclic Adenosine 3',5'-Monophosphate by Relaxin: B.T. Nguyen, et al. ; Mol. Endocrinol.
17, 1075 (2003),
Application(s): EIA using human culture supernatants & cell lysates,
Abstract;
D2-like receptor-mediated inhibition of Na+-K+-ATPase activity is dependent on the opening of K+ channels: P. Gomes, et al. ; Ren. Physiol.
283, F114 (2002),
Application(s): EIA using opossum cell lysates,
Abstract;
Renal responses to plasma volume expansion and hyperosmolality in fasting seal pups: R.M. Ortiz, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol.
282, R805 (2002),
Application(s): EIA using seal urine,
Abstract;
Urocortin, But Not Urocortin II, Protects Cultured Hippocampal Neurons from Oxidative and Excitotoxic Cell Death via Corticotropin-Releasing Hormone Receptor Type I: W.A. Pedersen, et al. ; J. Neurosci.
22, 404 (2002),
Application(s): EIA using rat cell lysates,
Abstract;
The Involvement of Tyrosine Kinases, Cyclic AMP/Protein Kinase A, and p38 Mitogen-Activated Protein Kinase in IL-13-Mediated Arginase I Induction in Macrophages: Its Implications in IL-13-Inhibited Nitric Oxide Production: C.I. Chang, et al. ; J. Immunol.
165, 2134 (2000),
Application(s): EIA using mouse cell lysates,
Abstract;
General Literature References
Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle
10, 8 (2011),
Abstract;
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