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IL-6 (human), high sensitivity ELISA kit

Most sensitive (0.057 pg/mL) colorimetric kit for IL-6 quantification
ENZ-KIT178-0001 96 wells 649.00 USD
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  • High-sensitivity ELISA enables quantification of low amounts of IL-6
  • Easy-to-use liquid color-coded reagents reduce errors
  • Rapid quantitative results in 3 hours
  • High-throughput immunoassay measures up to 40 samples in duplicate per 96 well kit
  • Ready-to-use reagents and pre-coated plates reduce errors and save time
The IL-6 (human), high sensitivity ELISA kit is a colorimetric immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Measure up to 40 samples in duplicate per 96 well  kit.
IL-6 (human), high sensitivity ELISA kit Parallelism curve
IL-6 (human), high sensitivity ELISA kit Standard curve
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IL-6 (human), high sensitivity ELISA kit Parallelism curve IL-6 (human), high sensitivity ELISA kit Standard curve

Product Details

Alternative Name:Interleukin-6
Sensitivity:0.057 pg/mL (1.56 - 50 pg/mL)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human IL-6 in culture supernatants, plasma, serum, and urine.
Wavelength:450 nm
Species reactivity:Human
Crossreactivity:Negligible crossreactivity to IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-7, IL-8, TNFα, and TNFβ
Use/Stability:Store all components at +4°C, except standard at -20°C.
Shipping:Blue Ice
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Stop solution
Scientific Background:Interleukin-6 (IL-6) is a cytokine critical to the regulation of the immune and hematopoietic systems. Human IL-6 is expressed by T-cells, mast cells, monocytes, macrophages, fibroblasts, endothelial cells, keratinocytes, and many tumor cell lines. It plays a role in acute phase reactions and response to injury and inflammation. Human IL-6 also stimulates the differentiation of B-cells for antibody production, promotes expansion of activated T-cells, expands hematopoietic cell production, and induces the expression of acute phase proteins.
UniProt ID:P05231
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Inflammatory and oxidative stress markers in intracerebral hemorrhage: Relevance as prognostic markers for quantification of the edema volume: V. Rendevski, et al.; Brain Pathol. 33, e13106 (2023), Abstract; Full Text
Resveratrol has its antioxidant and anti-inflammatory protective mechanisms decreased in aging: M.A. Santos, et al.; Arch. Gerontol. Geriatr. 107, 104895 (2023), Abstract;
Anti-inflammatory properties of S53P4 bioactive glass implant material: F.N. Barrak, et al.; J. Dent. 127, 104296 (2022), Abstract;
BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res. 169, 105611 (2021), Abstract;
Infrared light therapy relieves TLR-4 dependent hyper-inflammation of the type induced by COVID-19: B. Aguida, et al.; Commun. Integr. Biol. 14, 200 (2021), Application(s): IL-6 quantification on HEK293 cells supernatant, Abstract; Full Text
Sterile-filtered saliva is a strong inducer of IL-6 and IL-8 in oral fibroblasts: B. Cvikl, et al.; Clin. Oral. Investig. 19, 385 (2015), Application(s): Cell culture Supernatant, Abstract;

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