- Validated in a variety of sample types
- Fast, accurate assay results in less than 30 minutes
- Convenient 96 well plate format for higher throughput.
The Glutathione Peroxidase activity kit is a convenient 96-well plate based assay for the higher throughput analysis of glutathione peroxidase activity in a variety of sample matrices in just 30 minutes.
Product Specification
| Alternative Name: | GPx |
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| Applications: | Colorimetric detection
Activity assay
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| Application Notes: | For the measurement of glutathione peroxidase in cell lysates, plasma, and tissue from any species. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | Microtiter plate, Assay buffer concentrate, Reaction mix, Glutathione reductase, GSSG |
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| Scientific Background: | Glutathione, a major intracellular non-protein thiol, protects against free radical damage by providing reducing equivalents for several key antioxidant enzymes. In addition, glutathione acts as a scavenger of hydroxyl radicals and nascent oxygen. Elevated glutathione levels are associated with a reduced rate of illness, higher levels of self-rated health, lower cholesterol, lower body mass index, and lower blood pressure among the elderly. Glutathione provides a primary defense system for the removal of oxidants in the brain. Studies have revealed a correlation between low glutathione levels and damage to neurons that manufacture dopamine, suggesting a link to Parkinson's disease. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Sumoylation-deficient Prdx6 gains protective function by amplifying enzymatic activity and stability and escapes oxidative stress-induced aberrant Sumoylation: B. Chhunchha, et al.; Cell Death Dis.
8, e2525 (2017),
Abstract;
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Mitochondrial peroxiredoxin-3 protects against hyperglycemia induced myocardial damage in Diabetic cardiomyopathy: S. Arkat, et al.; Free Radic. Biol. Med.
97, 489 (2016),
Abstract;
Reformulated meat products protect against ischemia-induced cardiac damage: M.C. Asensio-Lopez, et al.; Food Funct.
7, 992 (2016),
Abstract;
S‐Glutathionylation of Keap1: a new role for glutathione S‐transferase pi in neuronal protection: A.N. Carvalho, et al.; FEBS Lett.
590, 1455 (2016),
Abstract;
Differences in Redox Regulatory Systems in Human Lung and Liver Tumors Suggest Different Avenues for Therapy: R. Tobe, et al.; Cancers (Basel)
7, 2262 (2015),
Application(s): GPX activities measured in protein extracts ,
Abstract;
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ErbB2 overexpression upregulates antioxidant enzymes, reduces basal levels of reactive oxygen species, and protects against doxorubicin cardiotoxicity: F. Belmonte, et al.; Am. J. Physiol. Heart Circ. Physiol.
309, H1271 (2015),
Abstract;
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Defensive mechanism in cholangiocarcinoma cells against oxidative stress induced by chlorine e6-based photodynamic therapy: H.M. Lee, et al.; Drug Des. Devel. Ther.
8, 1451 (2014),
Abstract;
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Ets-1 global gene expression profile reveals associations with metabolism and oxidative stress in ovarian and breast cancers: M.L. Verschoor, et al.; Cancer Metab.
1, 17 (2013),
Abstract;
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Involvement of ferritin heavy chain in preventive effect of metformin against doxorubicin-induced cardiotoxicity: M.C. Asensio-Lopez, et al.; Free Radic. Biol. Med.
57, 188 (2013),
Abstract;
Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions: E.E. Philipp, et al.; PLoS One
7, e44621 (2012),
Abstract;
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