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BIOARRAY™ Terminal labeling kit with biotin-ddUTP for DNA probe array assays

ENZ-42630 25 Reactions 551.00 USD
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The BIOARRAY™ 3’-OH Terminal Labeling System is the gold standard end-labeling system for use with Affymetrix® DNA SNP, Resequencing and Prokaryotic microarrays.

The BIOARRAY™ Terminal Labeling Kit with Biotin-ddUTP has been developed for DNA probe array assays. This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyze the addition of a single biotin-ddUMP to the 3’-OH terminus of an amplified and fragmented target DNA molecule. A standard reaction will label up to 100 picomoles (equivalent to 1µg of a 30-nucleotide sequence). When DNA fragments are labeled at the 3’-OH terminus, the sequence bias that occurs with either nick translation or random priming is eliminated.

Product Details

Use/Stability:Stable for at least one year after receipt when stored as recommended.
Shipping:Dry Ice
Long Term Storage:-20°C
Contents: Biotin-ddUTP (100X), 25 µl
5X Reaction Buffer, 500 µlPotassium cacodylate buffer, pH 7, containing β-mercaptoethanol and stabilizer
10X CoCl2 Solution, 250 µl
Terminal Deoxynucleotide Transferase (50X), 50 µl in storage buffer
Regulatory Status:RUO - Research Use Only

Product Literature References

Identification of Small Molecule Inhibitors of Staphylococcus aureus RnpA: J.M. Colquhoun, et al.; Antibiotics (Basel) 8, 48 (2019), Abstract; Full Text
A diffusion model for the coordination of DNA replication in Schizosaccharomyces pombe: T. Pichugina, et al.; Sci. Rep. 6, 18757 (2016), Abstract; Full Text
Involvement of the eukaryote-like kinase-phosphatase system and a protein that interacts with penicillin-binding protein 5 in emergence of cephalosporin resistance in cephalosporin-sensitive class A penicillin-binding protein mutants in Enterococcus faecium: C. Desbonnet, et al.; MBio. 7, e02188 (2016), Abstract;
Microarray analysis to monitor bacterial cell wall homeostasis: H.J. Hong, et al.; Methods Mol. Biol. 1440, 31 (2016), Abstract;
Characterizing the interactions between a naturally primed immunoglobulin A and its conserved bacteroides thetaiotaomicron species-specific epitope in gnotobiotic mice: D.A. Peterson, et al.; J. Biol. Chem. 290, 12630 (2015), Abstract;
Small-molecule inhibitors of Staphylococcus aureus RnpA-mediated RNA turnover and tRNA processing: T.M. Eidem, et al.; Antimicrob. Agents Chemother. 59, 2016 (2015), Abstract;
The formation of Streptococcus mutans persisters induced by the quorum-sensing peptide pheromone is affected by the LexA regulator: V. Leung, et al.; J. Bacteriol. 197, 1083 (2015), Abstract;
Rhodobacter sphaeroides adaptation to high concentrations of cobalt ions requires energetic metabolism changes: M. Volpicella, et al.; FEMS Microbiol. Ecol. 88, 345 (2014), Abstract;
Transcriptional response of Enterococcus faecalis to sunlight: L.M. Sassoubre, et al.; J. Photochem. Photobiol. B. 130, 349 (2014), Abstract;
A novel phosphotransferase system of Streptococcus mutans is responsible for transport of carbohydrates with α-1,3 linkage: D. Ajdic, et al.; Mol. Oral Microbiol. 28, 114 (2013), Abstract; Full Text
Genome-wide identification of genes conferring energy related resistance to a synthetic antimicrobial peptide (Bac8c): E.C. Spindler, et al.; PLoS One 8, e55052 (2013), Abstract; Full Text
Multiple roles of RNase Y in Streptococcus pyogenes mRNA processing and degradation: Z. Chen, et al.; J. Bacteriol. 195, 2585 (2013), Abstract; Full Text
Noncanonical transforming growth factor β (TGFβ) signaling in cranial neural crest cells causes tongue muscle developmental defects: J. Iwata, et al.; J. Biol. Chem. 288, 29760 (2013), Abstract; Full Text
O-antigen protects gram-negative bacteria from histone killing: C. Chaput, et al.; PLoS One 8, e71097 (2013), Abstract; Full Text
The multi-targeted effects of Chrysanthemum herb extract against Escherichia coli O157:H7: K.S. Kim, et al.; Phytother. Res. 27, 1398 (2013), Abstract;

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