Highly sensitive measurements, detecting as low as 1 ng/ml
Reliable and validated in-house in a variety of sample matrices
Fully quantitative results that surpass semi-quantitative Western blot analysis
No edge effect allowing you to assay more samples per kit
The DNA Damage EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 2.5 hours. This kit has been validated in-house in a variety of sample matrices and is supplied in a convenient 96 well plate format. Protocol (xls) available upon request.
Standard curve of DNA damage ELISA kit
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Product Details
Sensitivity:
0.59 ng/ml (range 0.94 - 60 ng/ml)
Assay Time:
<2.5 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the measurement of DNA Damage (8-OHdG) in serum, saliva, and urine from any species. Cited sample types include plasma, seminal fluids, cell lysates, culture supernatants and DNA extracts.
Wavelength:
450 nm
Species reactivity:
Species independent
Crossreactivity:
The DNA Damage ELISA also detects 8-hydroxyguanosine (product of oxidative RNA damage) and 8-hydroxyguanine (product of oxidative DNA damage by hydroxyl radicals).
Use/Stability:
Store all components at 4°C, except standard at -20°C.
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage.
Proteomic profiling and ROC analysis identify CD151 and ELAVL1 as potential therapy response markers for the antiviral drug in resistant TNBC: R. Marni, et al.; Life Sci. 320, 121534 (2023), Abstract;
The Dietary Fermentable Fiber Inulin Alters the Intestinal Microbiome and Improves Chronic Kidney Disease Mineral-Bone Disorder in a Rat Model of CKD: A. Biruete, et al.; bioRxiv (2023), Abstract;
Octanoic Acid - An Insecticidal Metabolite of Conidiobolus coronatus (Entomopthorales) That Affects Two Majors Antifungal Protection Systems in Galleria mellonella (Lepidoptera): Cuticular Lipids and Hemocyte: A. Kaczmarek, et al.; Int. J. Mol. Sci. 23, 5204 (2022), Abstract;
Wasp Venom Ameliorates Scopolamine-Induced Learning and Memory Impairment in Mice: J.H. Chae, et al.; Toxins 14, 256 (2022), Abstract;
Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury: M. Kumar, et al.; Biochem. Biophys. Res. Commun. 577, 52 (2021), Abstract;
Berberine ameliorates renal injury in a rat model of D-galactose-induced aging through a PTEN/Akt-dependent mechanism: H.E. El-Horany, et al.; Arch. Physiol. Biochem. 126, 157 (2020), Application(s): Rat blood serum, Abstract;
Evaluation of Effectiveness of Ultraviolet Emitting Lamps on the Cutaneous Production of Vitamin D3: Relationship of the Lamps Vitamin D3 Producing Potential to the Production of 8-Hydroxy-2'-Deoxyguanosine and Nitric Oxide: A. Shirvani, et al.; Anticancer Res. 40, 565 (2020), Abstract;
In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit: Y. Kim, et al.; Foods 9, 1033 (2020), Application(s): Mouse Liver tissue homogenate, Abstract; Full Text
Inhibitory Effect of Steamed Soybean Wastewater Against DSS-Induced Intestinal Inflammation in Mice: S. Jeong, et al.; Foods 9, 954 (2020), Application(s): Quantification of 8-OHdG level in mouse plasma., Abstract; Full Text
N-acetylcysteine (NAC), an anti-oxidant, does not improve bone mechanical properties in a rat model of progressive chronic kidney disease-mineral bone disorder: M.R. Allen, et al.; PLoS One 15, e0230379 (2020), Abstract; Full Text
Neuroprotective Effects of Euonymus alatus Extract on Scopolamine-Induced Memory Deficits in Mice: Y. Woo, et al.; Antioxidants (Basel) 9, 449 (2020), Application(s): Serum, Abstract; Full Text
Antioxidative effects of olive polyphenols compared to vitamin E in piglets fed a diet rich in N-3 PUFA: J. Leskovec, et al.; Animals (Basel) 9, 161 (2019), Application(s): ELISA using pig serum, Abstract; Full Text
MicroRNA-204-3p Attenuates High Glucose-Induced MPC5 Podocytes Apoptosis by Targeting Braykinin B2 Receptor: X. Han, et al.; Exp. Clin. Endocrinol. Diabetes 127, 387 (2019), Application(s): Mouse MPC5 podocyte lysates, Abstract;
Oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress: A. Stier, et al.; Sci. Rep. 9, 8545 (2019), Application(s): Penguin plasma and red blood cell lysate, Abstract; Full Text
Effects of Valproate Monotherapy on the Oxidant-Antioxidant Status in Mexican Epileptic Children: A Longitudinal Study: E. Beltrán-Sarmiento, et al.; Oxid. Med. Cell. Longev. 2018, 7954371 (2018), Application(s): Human blood plasma, Abstract; Full Text
Fibroblast growth factor 23 does not directly influence skeletal muscle cell proliferation and differentiation or ex vivo muscle contractility: K.G. Avin, et al.; Am. J. Physiol. Endocrinol. Metab. 315, E594 (2018), Application(s): Supernatant of C2C12 mouse myoblast cells, Abstract;
Mangiferin induces radiosensitization in glioblastoma cells by inhibiting nonhomologous end joining: F. Mu, et al.; Oncol. Rep. 40, 3663 (2018), Abstract;
miR-370 promotes high glucose-induced podocyte injuries by inhibiting angiotensin II type 1 receptor-associated protein: Y. Xian, et al.; Cell Biol. Int. 42, 1545 (2018), Application(s): MPC5 mouse podocyte lysates, Abstract;
Nuclear GSK3β induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastoma: Y. Yang, et al.; Int. J. Oncol. 52, 709 (2018), Application(s): ELISA using cell lysates, Abstract; Full Text
Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses: Z. Zhang, et al.; Mol. Med. Rep. 17, 7142 (2018), Application(s): ELISA using mouse plasma, Abstract; Full Text
Plasma concentrations of 8-hydroxy-2′-deoxyguanosine and risk of kidney disease and death in individuals with type 1 diabetes: M. Sanchez, et al.; Diabetologia 61, 977 (2018), Application(s): ELISA using human plasma, Abstract;
Protective Effects of Dioscorea batatas Flesh and Peel Extracts against Ethanol-Induced Gastric Ulcer in Mice: S. Byeon, et al.; Nutrients 10, 1680 (2018), Application(s): Mouse blood plasma, Abstract; Full Text
Proteomic Screen of Brain Glycoproteome Reveals Prion Specific Marker of Pathogenesis: L. Lamoureux, et al.; Proteomics 18, (2018), Application(s): Mouse urine, Abstract;
The cost of reproduction in women: reproductive effort and oxidative stress in premenopausal and postmenopausal American women: A. Ziomkiewicz, et al.; Am. J. Hum. Biol. 30, e23069 (2018), Application(s): ELISA using human serum, Abstract;
Effects of Zinc Supplementation on DNA Damage in Rats with Experimental Kidney Deficiency: S.Ç. Yegin, et al.; Biol. Trace Elem. Res. 176, 338 (2017), Application(s): Rat blood serum, Abstract;
Environmental conditions can modulate the links among oxidative stress, age, and longevity: V. Marasco, et al.; Mech. Ageing. Dev. 164, 100 (2017), Application(s): ELISA using bird plasma, Abstract;
Protective effect of glyceollins in a mouse model of dextran sulfate sodium-induced colitis: H. Seo, et al.; J. Med. Food. 20, 1055 (2017), Abstract;
Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses: Z. Jiang, et al.; Mol. Med. Rep. 16, 5219 (2017), Application(s): ELISA using human cell lysates, Abstract; Full Text
The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats: A. Usta & S. Dede; Pharmacogn Mag 13, 458 (2017), Application(s): Rat blood serum, Abstract; Full Text
Effects of Moderate Aerobic Exercise on Cognitive Abilities and Redox State Biomarkers in Older Adults: A.H. Alghadir, et al.; Oxid. Med. Cell. Longev. 2016, Article ID 2545168 (2016), Application(s): Immunoassay technique was performed to estimate serum8-OHdGas a marker of DNA damage, Abstract; Full Text
Evidence for the cost of reproduction in humans: high lifetime reproductive effort is associated with greater oxidative stress in post-menopausal women: A. Ziomkiewicz, et al.; PLoS One. 11, e0145753 (2016), Application(s): ELISA using human urine, Abstract;
Joint association of fruit and heterocyclic amine intake with DNA damage levels in a general population: A.M. de Carvalho, et al. ; Nutrition 32, 260 (2016), Application(s): ELISA measuring DNA damage in humans, Abstract;
Panax ginseng Fraction F3 Extracted by Supercritical Carbon Dioxide Protects against Oxidative Stress in ARPE-19 Cells: C.C. Yang, et al.; Int. J. Mol. Sci. 17, e1717 (2016), Application(s): Effects of P. ginseng F3 Fraction on Cellular Oxidation Induced by H2O2 in ARPE-19 Cells, Abstract; Full Text
Serum levels of 8-hydroxy-2'-deoxyguanosine DNA in patients with breast cancer and endometrial cancer with and without diabetes mellitus: L.M. Berstein, et al.; Bull. Exp. Biol. Med. 161, 547 (2016), Abstract;
Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney Disease: K.G. Avin, et al.; PLoS One 11, e0159411 (2016), Application(s): Plasma oxidative stress assay and myostatin assay, Abstract; Full Text
The Dose-Dependent Organ-Specific Effects of a Dipeptidyl Peptidase-4 Inhibitor on Cardiovascular Complications in a Model of Type 2 Diabetes: J.Y. Moon, et al.; PLoS One 11, e0150745 (2016), Application(s): Measured urinary 8-OHdG levels, Abstract; Full Text
Association of oxidative stress biomarkers with adiposity and clinical staging in women with breast cancer: A.A. Carioca, et al.; Eur. J. Clin. Nutr. 69, 1256 (2015), Application(s): ELISA using human plasma, Abstract;
Epigenetic Study of Parkinson’s Disease in Experimental Animal Model: A.F. Salama, et al.; Int. J. Clin. Exp. Neurology 3, 11 (2015), Application(s): ELISA, Full Text
Neuroprotective Role of Vitamin B3 in Experimentally Induced Oxidative Stress: A.E. Atrash, et al.; Int. J. Clin. Exp. Neurology 3, 21 (2015), Application(s): ELISA, Full Text
Oxidative stress and antioxidant responses to progressive resistance exercise intensity in trained and untrained males: H. Çakır-Atabek, et al.; Biol. Sport 32, 321 (2015), Application(s): ELSIA analysing 8-OHdG , Abstract; Full Text
Protective effects of alpha-lipoic acid against benzene induced toxicity in experimental rats: M.M. Batsh, et al.; Eur. Rev. Med. Pharmacol. Sci. 19, 2717 (2015), Application(s): ELISA using rat serum, Abstract; Full Text
Arterial stiffness, oxidative stress, and smoke exposure in wildland firefighters: D. Gaughan, et al.; Am. J. Ind. Med. 57, 748 (2014), Application(s): EIA using human urine, Abstract;
Discovery and characterization of artifactual mutations in deep coverage targeted capture sequencing data due to oxidative DNA damage during sample preparation: M. Costello, et al.; Nucleic Acids Res. 41, e67 (2013), Application(s): ELISA using DNA extracts, Abstract; Full Text
Genetic damage in patients with chronic kidney disease, peritoneal dialysis and haemodialysis: a comparative study: A. Rangel-López, et al.; Mutagenesis 28, 219 (2013), Application(s): ELISA using human serum, Abstract; Full Text
The antioxidant activity of copper (II)(3, 5-diisopropyl salicylate) 4 and its protective effect against streptozotocin-induced diabetes mellitus in rats: M. Qazzaz, et al.; Biol. Trace. Elem. Res. 154, 88 (2013), Application(s): EIA using rat serum, Abstract;
Cell therapy with human renal cell cultures containing erythropoietin-positive cells improves chronic kidney injury: L.M. Yamaleyeva, et al.; Stem Cells Transl. Med. 1, 373 (2012), Application(s): ELISA using rat urine, Abstract; Full Text
Evaluation of sperm DNA peroxidation in fertile and subfertile dogs: B.V. Lopes-Santiago, et al.; Reprod. Domest. Anim. 47, 208 (2012), Application(s): ELISA using dog seminal fluids, Abstract;
Increased oxidative DNA damage and impaired antioxidant defense system in patients with gastrointestinal cancer: A. Gönenç, et al.; Eur. J. Intern. Med. 23, 350 (2012), Application(s): ELISA using human serum, Abstract;
Comparison of the influence of four classes of HIV antiretrovirals on adipogenic differentiation: the minimal effect of raltegravir and atazanavir: R. Minami, et al.; J. Infect. Chemother. 17, 183 (2011), Application(s): ELISA using culture supernatants, Abstract;
Contrary effects of the receptor tyrosine kinase inhibitor vandetanib on constitutive and flow-stimulated nitric oxide elaboration in humans: E.L. Mayer, et al.; Hypertension 58, 85 (2011), Application(s): ELISA using human plasma, Abstract; Full Text
In vivo antioxidant potential of Sweet chestnut (Castanea sativa Mill.) wood extract in young growing pigs exposed to n-3 PUFA-induced oxidative stress: T. Frankic, J. Salobir; J. Sci. Food. Agric. 91, 1432 (2011), Application(s): EIA using pig urine , Abstract;
Lyophilized plasma reconstituted with ascorbic acid suppresses inflammation and oxidative DNA damage: P.Y. Van, et al.; J. Trauma 71, 20 (2011), Application(s): ELISA using pig serum, Abstract;
Radiation exposure differentially affects songbird 8-hydroxy-2'-deoxyguanosine plasma profiles: ionizing radiation damage response in songbirds: T.W. Luloff, et al.; Environ. Mol. Mutagen. 52, 658 (2011), Application(s): ELISA using bird plasma, Abstract;
The impact of OGG1, MTH1 and MnSOD gene polymorphisms on 8-hydroxy-2′-deoxyguanosine and cellular superoxide dismutase activity in myocardial ischemia–reperfusion: B. Karahalil, et al.; Mol. Biol. Rep. 38, 2427 (2011), Application(s): EIA using human serum, Abstract;
A radiation-induced adaptive response prolongs the survival of prion-infected mice: M. Plews, et al.; Free. Radic. Biol. Med. 49, 1417 (2010), Application(s): EIA using mouse urine, Abstract;
Altered salivary redox homeostasis in patients with systemic sclerosis: H. Su, et al.; J. Rheumatol. 37, 1858 (2010), Application(s): ELISA using human saliva, Abstract;
Antimutagenic activity of mitochondria-targeted plastoquinone derivative: V.A. Chistyakov, et al.; Biochemistry (Mosc) 75, 269 (2010), Application(s): ELISA using rat serum, Abstract; Full Text
Antioxidant enzyme activities and DNA damage in children with type 1 diabetes mellitus after 12 weeks of exercise: J. Woo, et al.; Acta Paediatr. 99, 1263 (2010), Application(s): ELISA using human serum, Abstract;
Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study: J. Shen, et al.; Am. J. Clin. Nutr. 91, 337 (2010), Application(s): EIA using human urine, Abstract; Full Text
MAT1A variants are associated with hypertension, stroke, and markers of DNA damage and are modulated by plasma vitamin B-6 and folate: C.Q. Lai, et al.; Am. J. Clin. Nutr. 91, 1377 (2010), Application(s): ELISA using human urine, Abstract; Full Text
The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs: T. Frankic, et al.; Animal 4, 572 (2010), Application(s): EIA using pig urine , Abstract; Full Text
The teratogenicity and behavioral teratogenicity of di(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) in a chick model: S. Abdul-Ghani, et al.; Neurotoxicol. Teratol. 34, 56 (2010), Application(s): EIA using chick serum, Abstract;
Ultraviolet A regulates adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells via up-regulation of Kruppel-like factor 2: J. Lee, et al.; J. Biol. Chem. 285, 32467 (2010), Application(s): ELISA using cell lysates, Abstract; Full Text
Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication: H.W. Gil, et al.; Clin. Toxicol. (Phila) 47, 870 (2009), Application(s): ELISA using human urine, Abstract;
Salivary DNA, lipid, and protein oxidation in nonsmokers with periodontal disease: H. Su, et al.; Free Radic. Biol. Med. 46, 914 (2009), Application(s): ELISA using human saliva, Abstract;
The comparison of in vivo antigenotoxic and antioxidativecapacity of two propylene glycol extracts of Calendulaofficinalis (marigold) and vitamin E in young growing pigs: T. Frankic, et al.; J. Anim. Physiol. Anim. Nutr. (Berl.) 93, 688 (2009), Application(s): EIA using pig urine , Abstract;
Multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other fluorescent probes (blue or cyan)
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
