One-step, ready-to-use solution with no radioisotopes
High sensitivity that correlates with the [3H]-thymidine incorporation assay
High-throughput screening without a solubilization step
More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.
It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
CCK-8 sensitivity for HeLa and HL60 cells are more sensitive than MTT
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Product Details
Alternative Name:
CCKi-8
Applications:
Colorimetric detection
Quantity:
100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL
Handling:
Protect from light. Avoid freeze/thaw cycles.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Contents:
WST-8 solution, 1-methoxy-PMS
Technical Info/Product Notes:
Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium.
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Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells: Y.C. Yoon, et al.; Molecules 20, 22128 (2015), Application(s): Determined the viability of cells, Abstract; Full Text
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