Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Cell Counting Kit-8

Colorimetric kit for simple and accurate cell proliferation and cytotoxicity assays.
ALX-850-039-0100 100 tests 81.00 USD
ALX-850-039-KI01 500 tests 195.00 USD
ALX-850-039-KI02 5x500 tests 588.00 USD
Do you need bulk/larger quantities?
  • One-step, ready-to-use solution with no radioisotopes
  • High sensitivity that correlates with the [3H]-thymidine incorporation assay
  • High-throughput screening without a solubilization step
  • More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.

It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
Cell Counting Kit-8 Fig7
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
Cell Counting Kit-8 Fig6
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
Cell Counting Kit-8 web_Fig1
Cell Counting Kit-8 091517_reformat
CCK-8 sensitivity for HeLa and HL60 cells are more sensitive than MTT
Please mouse over
Cell Counting Kit-8 Fig7 Cell Counting Kit-8 Fig6 Cell Counting Kit-8 web_Fig1 Cell Counting Kit-8 091517_reformat

Product Specification

Alternative Name:CCKi-8
Applications:Colorimetric detection
Quantity:100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL
Handling:Protect from light. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Contents:WST-8 solution, 1-methoxy-PMS
Technical Info/Product Notes:

Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium.

Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal

Product Literature References

A simultaneous grafting/vinyl polymerization process generates a polycationic surface for enhanced antibacterial activity of bacterial cellulose: H. Liu, et al.; Int. J. Biol. Macromol. 143, 224 (2020), Abstract;
Coating M-CSF on plastic surface results in the generation of increased numbers of macrophages in vitro: S. Sadh, et al.; J. Immunol. Methods 481-482, 112788 (2020), Abstract;
Curcumol Suppresses Triple-negative Breast Cancer Metastasis by Attenuating Anoikis Resistance via Inhibition of Skp2-mediated Transcriptional Addiction: C.L. Li, et al.; Anticancer Res. 40, 5529 (2020), Abstract;
Cytotoxicity and the Effect of Temperature on Physical Properties and Chemical Composition of a New Calcium Silicate-based Root Canal Sealer: B. Chen, et al.; J. Endod. 46, 531 (2020), Application(s): Viability of human periodontal ligament fibroblasts., Abstract;
Decellularized liver matrix as substrates for rescue of acute hepatocytes toxicity: Y.T. Hou, et al.; J. Biomed. Mater. Res. B. Appl. Biomater. 108, 1592 (2020), Abstract;
Development of a 3D porous chitosan/gelatin liver scaffold for a bioartificial liver device: Y.T. Hou, et al.; J. Biosci. Bioeng. 129, 741 (2020), Abstract;
In vivo epigenetic CRISPR screen identifies Asf1a as an immunotherapeutic target in Kras-mutant lung adenocarcinoma: F. Li, et al.; Cancer Discov. 10, 270 (2020), Abstract;
Polarization of M2 Macrophages by Interaction between Prostate Cancer Cells Treated with Trichomonas vaginalis and Adipocytes: H.Y. Chung, et al.; Korean J. Parasitol. 58, 217 (2020), Application(s): Proliferation of prostate cancer cells (TRAMP-C2)., Abstract; Full Text
Tristetraprolin regulates necroptosis during tonic Toll-like receptor 4 (TLR4) signaling in murine macrophages: A. Ariana, et al.; J. Biol. Chem. (2020), Abstract; Full Text
Alterations in IL-4, IL-10 and IFN-γ levels synergistically decrease lipid content and protein expression of FAS and mature SREBP-1 in human sebocytes: J. Shin, et al.; Arch. Dermatol. Res. 311, 563 (2019), Abstract;
Effect of strontium doping on the biocompatibility of calcium phosphate-coated titanium substrates: T.T. Nguyen, et al.; J. Appl. Biomater. Funct. Mater. 17, 2280800019826517 (2019), Abstract; Full Text
Expression of TMBIM6 in Cancers: The Involvement of Sp1 and PKC: R.P. Junjappaa, et al.; Cancers 11, 974 (2019), Abstract; Full Text
Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology: S.M. Ahmed, et al.; Mol. Oncol. 13, 2062 (2019), Abstract; Full Text
The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity: S.M. Ahmed, et al.; PLoS One. 14, e0215696 (2019), Abstract; Full Text
Characterization of minoxidil/hydroxypropyl-β-cyclodextrin inclusion complex in aqueous alginate gel useful for alopecia management: Efficacy evaluation in male rat: D. Tricarico, et al.; Eur. J. Pharmacol. 122, 146 (2018), Abstract;
Gomisin A Suppresses Colorectal Lung Metastasis by Inducing AMPK/p38-Mediated Apoptosis and Decreasing Metastatic Abilities of Colorectal Cancer Cells: J.K. Kee, et al.; Front. Pharmacol. 9, 986 (2018), Abstract; Full Text
Honokiol inhibits in vitro and in vivo growth of oral squamous cell carcinoma through induction of apoptosis, cell cycle arrest and autophagy: K.J. Huang, et al.; J. Cell. Mol. Med. 22, 1894 (2018), Abstract;
LncRNA snaR upregulates GRB2-associated binding protein 2 and promotes proliferation of ovarian carcinoma cells: Y. Huang, et al.; Biochem. Biophys. Res. Commun. 503, 2028 (2018), Abstract;
NF-κB signaling is key in the wound healing processes of silk fibroin: Y.R. Park, et al.; Acta Biomater. 67, 183 (2018), Abstract;
Role of Klf4 in the regulation of apoptosis and cell cycle in rat granulosa cells during the periovulatory period: H. Choi, et al.; Int. J. Mol. Sci. 20, 87 (2018), Abstract; Full Text
Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation: M.C. Moorer, et al.; J. Cell. Sci. 130, 531 (2017), Application(s): Cell proliferation using bone marrow stromal cells (BMSC), Abstract; Full Text
The influence of oxygen concentration on the extracellular matrix production of human nucleus pulposus cells during isolation‐expansion process: S.H. Yang, et al.; J. Biomed. Mater. Res. A 105, 1575 (2017), Abstract;
ZEB1 is neither sufficient nor required for epithelial-mesenchymal transition in LS174T colorectal cancer cells: S. Jägle, et al.; Biochem. Biophys. Res. Commun. 482, 1226 (2017), Abstract;
Effect of EPHA2 gene mutation on congenital cataract and its underlying mechanism: Q. Dai, et al.; Int. J. Clin. Exp. Pathol. 9, 11099 (2016), Full Text
Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture: F. Angelini, et al.; Bioimpacts 6, 15 (2016), Application(s): WST-assay (cell proliferation and cytotoxicity assays), Abstract; Full Text
In vitro inhibition of Toxoplasma gondii by the anti-malarial candidate, 6-(1,2,6,7-tetraoxaspiro[7.11]nonadec-4-yl)hexan-1-ol: C.F. Xin, et al.; Parasitol. Int. 16, 30212 (2016), Application(s): Cell viability analysis to measure anti-T. gondii activity and cytotoxicity in vitro, Abstract;
Inhibitory effect of quercetin on colorectal lung metastasis through inducing apoptosis, and suppression of metastatic ability: J.Y. Kee, et al.; Phytomedicine 23, 1680 (2016), Application(s): WST assay, mouse colon carcinoma cell line , Abstract;
The level of nitric oxide regulates lipocalin-2 expression under inflammatory condition in RINm5F beta-cells: S.Y. Chang, et al.; Biochem. Biophys. Res. Commun. 476, 7 (2016), Application(s): Cell viability assay, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Cell viability assay; Effect of VD2 and Aβ25–35 induction on BV2 cells, Abstract;
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells: J.H. Kim, et al.; Int. J. Nanomedicine 10, 5513 (2015), Application(s): Cytotoxicity measurement, Abstract; Full Text
Circulating Pneumolysin Is a Potent Inducer of Cardiac Injury during Pneumococcal Infection: Y. Alhamdi, et al.; PLoS Pathog. 11, e1004836 (2015), Application(s): Assay using murine cardiomyocytes, Abstract; Full Text
Fabrication of Duck's feet collagen-Silk hybrid biomaterial for Tissue Engineering: S.H. Kim, et al.; Int. J. of Biol. Macromol. 85, 442 (2015), Application(s): Cytotoxicity test, Abstract;
Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells: Y.C. Yoon, et al.; Molecules 20, 22128 (2015), Application(s): Determined the viability of cells, Abstract; Full Text
ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells: S.G. Tilson, et al.; PLoS One 10, e0132823 (2015), Application(s): Toxicity Assay, Abstract; Full Text
The anti-inflammatory effect of prunus yedoensis bark extract on adipose tissue in diet-induced obese mice: H. Kang, et al.; Evid. Based Complement. Alternat. Med. 2015, Article ID 937904 (2015), Application(s): Cell viability assay, Abstract; Full Text
3D silk fibroin scaffold incorporating titanium dioxide (TiO2) nanoparticle (NPs) for tissue engineering: J.H. Kim, et al.; Int. J. Biol. Macromol. 68, 158 (2014), Abstract;
Dietary fat sources differentially modulate intestinal barrier and hepatic inflammation in alcohol-induced liver injury in rats: W. Zhong, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 305, G919 (2013), Abstract; Full Text
Hypoxia-inducible factor (HIF)-1α suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction: P. Storti, et al.; Leukemia 27, 1697 (2013), Abstract;
Solomonsterol A, a marine pregnane-X-receptor agonist, attenuates inflammation and immune dysfunction in a mouse model of arthritis: A. Mencarelli, et al.; Mar. Drugs 12, 36 (2013), Abstract; Full Text
Increased osteocyte death in multiple myeloma patients: role in myeloma-induced osteoclast formation: N. Giuliani, et al.; Leukemia 26, 1391 (2012), Abstract;
Biobased poly(propylene sebacate) as shape memory polymer with tunable switching temperature for potential biomedical applications: B. Guo, et al.; Biomacromolecules 12, 1312 (2011), Abstract; Full Text
Circulating Toll-like receptor (TLR) 2, TLR4, and regulatory T cells in patients with chronic hepatitis C: J.P. Wang, et al.; APMIS 118, 261 (2010), Abstract;
Effect of proteasome inhibitors with different chemical structures on the ubiquitin-proteasome system in vitro: V. Csizmadia, et al.; Vet. Pathol. 47, 358 (2010), Abstract;
Markers of tumor-initiating cells predict chemoresistance in breast cancer: C. Gong, et al.; PLoS One 5, e15630 (2010), Abstract; Full Text
Overexpression of Toll-like receptor 2/4 on monocytes modulates the activities of CD4(+)CD25(+) regulatory T cells in chronic hepatitis B virus infection: Y. Zhang, et al.; Virology 397, 34 (2010), Abstract;
Synthesis, properties and in vitro photodynamic activity of water-soluble azaphthalocyanines and azanaphthalocyanines: P. Zimcik, et al.; Photochem. Photobiol. 86, 168 (2010), Abstract;
COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells: H. Radilova, et al.; Mol. Cell Biochem. 330, 131 (2009), Abstract;
Interleukin-1beta increases gap junctional communication among synovial fibroblasts via the extracellular-signal-regulated kinase pathway: C. Niger, et al.; Biol. Cell 102, 37 (2009), Abstract; Full Text
Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect: Y. Kim, et al.; J. Biol. Chem. 280, 23273 (2005), Abstract; Full Text
Cyclin G2 dysregulation in human oral cancer: Y. Kim, et al.; Cancer Res. 64, 8980 (2004), Abstract; Full Text
Mitogen-activated protein kinases mediate peroxynitrite-induced cell death in human bronchial epithelial cells: E. Nabeyrat, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 284, L1112 (2003), Abstract; Full Text
Increased cytotoxicity of cadmium in fibroblasts lacking c-fos: M. Matsuoka, et al.; Biochem. Pharmacol. 59, 1573 (2000), Abstract;
A water-soluble tetrazolium salt useful for colorimetric cell viability assay: H. Tominaga, et al.; Anal. Commun. 36, 47 (1999),
Enhancement of MTT, a tetrazolium salt, exocytosis by amyloid beta- protein and chloroquine in cultured rat astrocytes: I. Isobe, et al.; Neurosci. Lett. 266, 129 (1999), Abstract;
A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability: M. Ishiyama, et al.; Talanta 44, 1299 (1997),
Antenatal hydronephrosis. Fetal and neonatal management: J.S. Elder; Pediatr. Clin. North Am. 44, 1299 (1997), Abstract;
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet: M. Ishiyama, et al.; Biol. Pharm. Bull. 19, 1518 (1996), Abstract;

Related Products

Erk1/2 ELISA kit 

ELISA, Colorimetric detection | Print as PDF
ADI-900-152 96 wells 402.00 USD
Do you need bulk/larger quantities?

[pThr202/Tyr204]Erk1/2 ELISA kit 

Sensitive ELISA kit for this key cell signaling molecule for kinase, proliferation, apoptosis, and differentiation research.
ELISA, Colorimetric detection | Print as PDF
ADI-900-098A-0001 96 wells 690.00 USD
Do you need bulk/larger quantities?

IGF-1 (human), ELISA kit 

Highly sensitive ELISA, measuring as little as 34.2 pg/ml of IGF-1 in just 4 hours.
ELISA, Colorimetric detection | Print as PDF
ADI-900-150 96 wells 585.00 USD
Do you need bulk/larger quantities?

TGF-β1 ELISA kit 

ELISA, Colorimetric detection | Print as PDF
ADI-900-155 96 wells 529.00 USD
Do you need bulk/larger quantities?

Tyrphostin AG 1024 

IGF-1 inhibitor
65678-07-1, ≥98% (HPLC (UV)) | Print as PDF
ALX-270-217-M001 1 mg 88.00 USD
ALX-270-217-M005 5 mg 325.00 USD
Do you need bulk/larger quantities?

Reveromycin D 

Cell cycle inhibitor
144860-70-8, Isolated from Streptomyces sp. MST-AS5344., ≥95% (HPLC) | Print as PDF
ALX-380-219-MC25 0.25 mg 251.00 USD
Do you need bulk/larger quantities?

NUCLEAR-ID® Red cell cycle kit (GFP-CERTIFIED®) 

Convenient kit for studying cell cycle progression by various applications
Flow Cytometry, Fluorescence microscopy | Print as PDF
ENZ-51008-100 1 Kit 369.00 USD
Do you need bulk/larger quantities?

LDH Cytotoxicity WST Assay 

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity
Colorimetric detection | Print as PDF
ENZ-KIT157-0500 500 tests 221.00 USD
ENZ-KIT157-2500 5x500 tests 830.00 USD
Do you need bulk/larger quantities?

Absorbance 96 Plate Reader 

Personal, Compact Plate Reader
ELISA, Colorimetric detection | Print as PDF
ENZ-INS-A96 1 ea Inquire for pricing

Product Toolbox


Certificate of Analysis


By catalog section:


Technical Service
Customer Service

Related Literature

Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

All new literature pieces

Recommend this page

Keep in touch

©2021 Enzo Life Sciences, Inc.,