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Cell Counting Kit-8

Colorimetric kit for simple and accurate cell proliferation and cytotoxicity assays.
ALX-850-039-0100 100 tests 93.00 USD
ALX-850-039-KI01 500 tests 210.00 USD
ALX-850-039-KI02 5x500 tests 635.00 USD
Do you need bulk/larger quantities?
  • One-step, ready-to-use solution with no radioisotopes
  • High sensitivity that correlates with the [3H]-thymidine incorporation assay
  • High-throughput screening without a solubilization step
  • More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.

It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
Cell Counting Kit-8 Fig7
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
Cell Counting Kit-8 Fig6
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
Cell Counting Kit-8 web_Fig1
Cell Counting Kit-8 091517_reformat
CCK-8 sensitivity for HeLa and HL60 cells are more sensitive than MTT
Please mouse over
Cell Counting Kit-8 Fig7 Cell Counting Kit-8 Fig6 Cell Counting Kit-8 web_Fig1 Cell Counting Kit-8 091517_reformat

Product Details

Alternative Name:CCKi-8
Applications:Colorimetric detection
Quantity:100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL
Handling:Protect from light. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Contents:WST-8 solution, 1-methoxy-PMS
Technical Info/Product Notes:

Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium.

Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

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ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells: S.G. Tilson, et al.; PLoS One 10, e0132823 (2015), Application(s): Toxicity Assay, Abstract; Full Text
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