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Cell Counting Kit-8

Simple, fast and accurate assay to measure cell proliferation and cytotoxicity
ALX-850-039-0100 100 tests 100.00 USD
ALX-850-039-KI01 500 tests 216.00 USD
ALX-850-039-KI02 5x500 tests 641.00 USD
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  • One-step, ready-to-use solution with no radioisotopes
  • High sensitivity that correlates with the [3H]-thymidine incorporation assay
  • High-throughput screening without a solubilization step
  • More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.

It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
Cell Counting Kit-8 Comparison table
Cell Counting kit-8 performance
Cell Counting Kit-8 Fig7
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
Cell Counting Kit-8 Fig6
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
Cell Counting Kit-8 web_Fig1
Cell Counting Kit-8 091517_reformat
CCK-8 sensitivity for HeLa and HL60 cells are more sensitive than MTT
Please mouse over
Cell Counting Kit-8 Comparison table Cell Counting Kit-8 Fig7 Cell Counting Kit-8 Fig6 Cell Counting Kit-8 web_Fig1 Cell Counting Kit-8 091517_reformat

Product Details

Alternative Name:CCKi-8
Applications:Colorimetric detection
Quantity:100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL
Handling:Protect from light. Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Contents:WST-8 solution, 1-methoxy-PMS
Technical Info/Product Notes:

Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium.

Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

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Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation: M.C. Moorer, et al.; J. Cell. Sci. 130, 531 (2017), Application(s): Cell proliferation using bone marrow stromal cells (BMSC), Abstract; Full Text
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ZEB1 is neither sufficient nor required for epithelial-mesenchymal transition in LS174T colorectal cancer cells: S. Jägle, et al.; Biochem. Biophys. Res. Commun. 482, 1226 (2017), Abstract;
Effect of EPHA2 gene mutation on congenital cataract and its underlying mechanism: Q. Dai, et al.; Int. J. Clin. Exp. Pathol. 9, 11099 (2016), Full Text
Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture: F. Angelini, et al.; Bioimpacts 6, 15 (2016), Application(s): WST-assay (cell proliferation and cytotoxicity assays), Abstract; Full Text
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The level of nitric oxide regulates lipocalin-2 expression under inflammatory condition in RINm5F beta-cells: S.Y. Chang, et al.; Biochem. Biophys. Res. Commun. 476, 7 (2016), Application(s): Cell viability assay, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Cell viability assay; Effect of VD2 and Aβ25–35 induction on BV2 cells, Abstract;
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Increased cytotoxicity of cadmium in fibroblasts lacking c-fos: M. Matsuoka, et al.; Biochem. Pharmacol. 59, 1573 (2000), Abstract;
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