One-step, ready-to-use solution with no radioisotopes
High sensitivity that correlates with the [3H]-thymidine incorporation assay
High-throughput screening without a solubilization step
More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.
It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
Cell Counting kit-8 performance
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
CCK-8 sensitivity for HeLa and HL60 cells are more sensitive than MTT
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Product Details
Alternative Name:
CCKi-8
Applications:
Colorimetric detection
Quantity:
100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL
Handling:
Protect from light. Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Contents:
WST-8 solution, 1-methoxy-PMS
Technical Info/Product Notes:
Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium.
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Synergistic effects of concurrent photodynamic therapy with indocyanine green and chemotherapy in hepatocellular carcinoma cell lines and mouse models: J.S. Park, et al.; J. Photochem. Photobiol. B 239, 112642 (2023), Abstract;
Synthesis and biological evaluation of novel N-benzyltriazolyl-hydroxamate derivatives as selective histone deacetylase 6 inhibitors: S.J. Kong, et al.; Bioorg. Med. Chem. 79, 117154 (2023), Abstract;
Control of hippocampal prothrombin kringle‐2 (pKr‐2) expression reduces neurotoxic symptoms in five familial Alzheimer's disease mice: S. Kim, et al.; Br. J. Pharmacol. 179, 998 (2022), Abstract;
Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading Capacity: A. Nair, et al.; Nanomaterials 12, 3 (2022), Abstract;
Enhancement of Biofunctionalization by Loading Manuka Oil on TiO2 Nanotubes: S.Y. Kim, et al.; Nanomaterials 12, 569 (2022), Abstract;
Human constitutive androstane receptor represses liver cancer development and hepatoma cell proliferation by inhibiting erythropoietin signaling: Z. Li, et al.; J. Biol. Chem. 298, 101885 (2022), Abstract;
Improvement of osseointegration of Ti–6Al–4V ELI alloy orthodontic mini-screws through anodization, cyclic pre-calcification, and heat treatments: C. Im, et al.; Prog. Orthog. 23, 11 (2022), Abstract;
lncRNA AGAP2-AS1 Facilitates Tumorigenesis and Ferroptosis Resistance through SLC7A11 by IGF2BP2 Pathway in Melanoma: L. An, et al.; Comput. Math. Methods Med. 2022, 1972516 (2022), Abstract;
lncRNA NUTM2A-AS1 Targets the SRSF1/Trim37 Signaling Pathway to Promote the Proliferation and Invasion of Breast Cancer: X. Ning, et al.; Comput. Math. Methods Med. 2022, 3299336 (2022), Abstract;
Loss of TSC1/TSC2 sensitizes immune checkpoint blockade in non-small cell lung cancer: Q. Huang, et al.; Sci. Adv. 8, eabi953 (2022), Abstract;
Mitochondrial metabolic reprogramming by SIRT3 regulation ameliorates drug resistance in renal cell carcinoma: Y.R. Gu, et al.; PLoS One 17, e0269432 (2022), Abstract;
Nitric Oxide-Releasing Gelatin Methacryloyl/Silk Fibroin Interpenetrating Polymer Network Hydrogels for Tissue Engineering Applications: S. Ghalei, et al.; ACS Biomater. Sci. Eng. 8, 273 (2022), Abstract;
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The effect of prolonged holding time on the mechanical property and microstructural property of lithium disilicate glass-ceramic: F. Lin, et al.; J. Mater. Sci. Med. 33, 69 (2022), Abstract;
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Therapeutic Effect of Melittin-dKLA Targeting Tumor-Associated Macrophages in Melanoma: I.H. Han, et al.; Int. J. Mol. Sci. 23, 3094 (2022), Abstract;
4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci. 37, 604 (2021), Application(s): Rheumatoid arthritis synovialfibroblasts (RASFSs) proliferation assay, Abstract;
Bioactive small molecules in calcium phosphate scaffold enhanced osteogenic differentiation of human induced pluripotent stem cells: B. Song, et al.; Dent. Mater. J. 40, 615 (2021), Abstract;
Cargo proteins in extracellular vesicles: potential for novel therapeutics in non-alcoholic steatohepatitis: J. Kim, et al.; J. Nanobiotechnology 19, 372 (2021), Abstract;
Effect of Slit/Robo signaling on regeneration in lung emphysema: J.S. Park, et al.; Exp. Mol. Med. 53, 986 (2021), Abstract; Full Text
Fabrication and Characterization of Biodegradable Gelatin Methacrylate/Biphasic Calcium Phosphate Composite Hydrogel for Bone Tissue Engineering: J.B. Choi, et al.; Nanomaterials 11, 617 (2021), Abstract; Full Text
Genetic and Functional Analysis of Glycosyltransferase 8 Domain–Containing Protein 1 in Taiwanese Patients With Amyotrophic Lateral Sclerosis: P.C. Tsai, et al.; Neurol. Genet. 7, e267 (2021), Abstract;
Overexpression of SIRT3 Suppresses Oxidative Stress-induced Neurotoxicity and Mitochondrial Dysfunction in Dopaminergic Neuronal Cells: S. Lee, et al.; Exp. Neurobiol. 30, 341 (2021), Abstract;
Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice: Y. C. Lee, et al.; Sci. Rep. 11, 21874 (2021), Abstract;
TrkB/C-induced HOXC6 activation enhances the ADAM8-mediated metastasis of chemoresistant colon cancer cells: G.B. Park, et al.; Mol. Med. Rep. 23, 423 (2021), Abstract;
Vitamin B12 Reduces TDP-43 Toxicity by Alleviating Oxidative Stress and Mitochondrial Dysfunction: Y.M. Jeon, et al.; Antioxidants 11, 82 (2021), Abstract;
A simultaneous grafting/vinyl polymerization process generates a polycationic surface for enhanced antibacterial activity of bacterial cellulose: H. Liu, et al.; Int. J. Biol. Macromol. 143, 224 (2020), Abstract;
Coating M-CSF on plastic surface results in the generation of increased numbers of macrophages in vitro: S. Sadh, et al.; J. Immunol. Methods 481-482, 112788 (2020), Abstract;
Curcumol Suppresses Triple-negative Breast Cancer Metastasis by Attenuating Anoikis Resistance via Inhibition of Skp2-mediated Transcriptional Addiction: C.L. Li, et al.; Anticancer Res. 40, 5529 (2020), Abstract;
Cytotoxicity and the Effect of Temperature on Physical Properties and Chemical Composition of a New Calcium Silicate-based Root Canal Sealer: B. Chen, et al.; J. Endod. 46, 531 (2020), Application(s): Viability of human periodontal ligament fibroblasts., Abstract;
Decellularized liver matrix as substrates for rescue of acute hepatocytes toxicity: Y.T. Hou, et al.; J. Biomed. Mater. Res. B. Appl. Biomater. 108, 1592 (2020), Abstract;
Development of a 3D porous chitosan/gelatin liver scaffold for a bioartificial liver device: Y.T. Hou, et al.; J. Biosci. Bioeng. 129, 741 (2020), Abstract;
In vivo epigenetic CRISPR screen identifies Asf1a as an immunotherapeutic target in Kras-mutant lung adenocarcinoma: F. Li, et al.; Cancer Discov. 10, 270 (2020), Abstract;
Polarization of M2 Macrophages by Interaction between Prostate Cancer Cells Treated with Trichomonas vaginalis and Adipocytes: H.Y. Chung, et al.; Korean J. Parasitol. 58, 217 (2020), Application(s): Proliferation of prostate cancer cells (TRAMP-C2)., Abstract; Full Text
Tristetraprolin regulates necroptosis during tonic Toll-like receptor 4 (TLR4) signaling in murine macrophages: A. Ariana, et al.; J. Biol. Chem. 295, 4661 (2020), Abstract; Full Text
Alterations in IL-4, IL-10 and IFN-γ levels synergistically decrease lipid content and protein expression of FAS and mature SREBP-1 in human sebocytes: J. Shin, et al.; Arch. Dermatol. Res. 311, 563 (2019), Abstract;
Effect of strontium doping on the biocompatibility of calcium phosphate-coated titanium substrates: T.T. Nguyen, et al.; J. Appl. Biomater. Funct. Mater. 17, 2280800019826517 (2019), Abstract; Full Text
Expression of TMBIM6 in Cancers: The Involvement of Sp1 and PKC: R.P. Junjappaa, et al.; Cancers 11, 974 (2019), Abstract; Full Text
Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology: S.M. Ahmed, et al.; Mol. Oncol. 13, 2062 (2019), Abstract; Full Text
The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity: S.M. Ahmed, et al.; PLoS One. 14, e0215696 (2019), Abstract; Full Text
Characterization of minoxidil/hydroxypropyl-β-cyclodextrin inclusion complex in aqueous alginate gel useful for alopecia management: Efficacy evaluation in male rat: D. Tricarico, et al.; Eur. J. Pharmacol. 122, 146 (2018), Abstract;
Gomisin A Suppresses Colorectal Lung Metastasis by Inducing AMPK/p38-Mediated Apoptosis and Decreasing Metastatic Abilities of Colorectal Cancer Cells: J.K. Kee, et al.; Front. Pharmacol. 9, 986 (2018), Abstract; Full Text
Honokiol inhibits in vitro and in vivo growth of oral squamous cell carcinoma through induction of apoptosis, cell cycle arrest and autophagy: K.J. Huang, et al.; J. Cell. Mol. Med. 22, 1894 (2018), Abstract;
LncRNA snaR upregulates GRB2-associated binding protein 2 and promotes proliferation of ovarian carcinoma cells: Y. Huang, et al.; Biochem. Biophys. Res. Commun. 503, 2028 (2018), Abstract;
NF-κB signaling is key in the wound healing processes of silk fibroin: Y.R. Park, et al.; Acta Biomater. 67, 183 (2018), Abstract;
Role of Klf4 in the regulation of apoptosis and cell cycle in rat granulosa cells during the periovulatory period: H. Choi, et al.; Int. J. Mol. Sci. 20, 87 (2018), Abstract; Full Text
Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation: M.C. Moorer, et al.; J. Cell. Sci. 130, 531 (2017), Application(s): Cell proliferation using bone marrow stromal cells (BMSC), Abstract; Full Text
The influence of oxygen concentration on the extracellular matrix production of human nucleus pulposus cells during isolation‐expansion process: S.H. Yang, et al.; J. Biomed. Mater. Res. A 105, 1575 (2017), Abstract;
ZEB1 is neither sufficient nor required for epithelial-mesenchymal transition in LS174T colorectal cancer cells: S. Jägle, et al.; Biochem. Biophys. Res. Commun. 482, 1226 (2017), Abstract;
Effect of EPHA2 gene mutation on congenital cataract and its underlying mechanism: Q. Dai, et al.; Int. J. Clin. Exp. Pathol. 9, 11099 (2016), Full Text
Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture: F. Angelini, et al.; Bioimpacts 6, 15 (2016), Application(s): WST-assay (cell proliferation and cytotoxicity assays), Abstract; Full Text
In vitro inhibition of Toxoplasma gondii by the anti-malarial candidate, 6-(1,2,6,7-tetraoxaspiro[7.11]nonadec-4-yl)hexan-1-ol: C.F. Xin, et al.; Parasitol. Int. 16, 30212 (2016), Application(s): Cell viability analysis to measure anti-T. gondii activity and cytotoxicity in vitro, Abstract;
Inhibitory effect of quercetin on colorectal lung metastasis through inducing apoptosis, and suppression of metastatic ability: J.Y. Kee, et al.; Phytomedicine 23, 1680 (2016), Application(s): WST assay, mouse colon carcinoma cell line , Abstract;
The level of nitric oxide regulates lipocalin-2 expression under inflammatory condition in RINm5F beta-cells: S.Y. Chang, et al.; Biochem. Biophys. Res. Commun. 476, 7 (2016), Application(s): Cell viability assay, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Cell viability assay; Effect of VD2 and Aβ25–35 induction on BV2 cells, Abstract;
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells: J.H. Kim, et al.; Int. J. Nanomedicine 10, 5513 (2015), Application(s): Cytotoxicity measurement, Abstract; Full Text
Circulating Pneumolysin Is a Potent Inducer of Cardiac Injury during Pneumococcal Infection: Y. Alhamdi, et al.; PLoS Pathog. 11, e1004836 (2015), Application(s): Assay using murine cardiomyocytes, Abstract; Full Text
Fabrication of Duck's feet collagen-Silk hybrid biomaterial for Tissue Engineering: S.H. Kim, et al.; Int. J. of Biol. Macromol. 85, 442 (2015), Application(s): Cytotoxicity test, Abstract;
Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells: Y.C. Yoon, et al.; Molecules 20, 22128 (2015), Application(s): Determined the viability of cells, Abstract; Full Text
ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells: S.G. Tilson, et al.; PLoS One 10, e0132823 (2015), Application(s): Toxicity Assay, Abstract; Full Text
The anti-inflammatory effect of prunus yedoensis bark extract on adipose tissue in diet-induced obese mice: H. Kang, et al.; Evid. Based Complement. Alternat. Med. 2015, Article ID 937904 (2015), Application(s): Cell viability assay, Abstract; Full Text
3D silk fibroin scaffold incorporating titanium dioxide (TiO2) nanoparticle (NPs) for tissue engineering: J.H. Kim, et al.; Int. J. Biol. Macromol. 68, 158 (2014), Abstract;
Dietary fat sources differentially modulate intestinal barrier and hepatic inflammation in alcohol-induced liver injury in rats: W. Zhong, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 305, G919 (2013), Abstract; Full Text
Hypoxia-inducible factor (HIF)-1α suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction: P. Storti, et al.; Leukemia 27, 1697 (2013), Abstract;
Solomonsterol A, a marine pregnane-X-receptor agonist, attenuates inflammation and immune dysfunction in a mouse model of arthritis: A. Mencarelli, et al.; Mar. Drugs 12, 36 (2013), Abstract; Full Text
Increased osteocyte death in multiple myeloma patients: role in myeloma-induced osteoclast formation: N. Giuliani, et al.; Leukemia 26, 1391 (2012), Abstract;
Biobased poly(propylene sebacate) as shape memory polymer with tunable switching temperature for potential biomedical applications: B. Guo, et al.; Biomacromolecules 12, 1312 (2011), Abstract; Full Text
Circulating Toll-like receptor (TLR) 2, TLR4, and regulatory T cells in patients with chronic hepatitis C: J.P. Wang, et al.; APMIS 118, 261 (2010), Abstract;
Effect of proteasome inhibitors with different chemical structures on the ubiquitin-proteasome system in vitro: V. Csizmadia, et al.; Vet. Pathol. 47, 358 (2010), Abstract;
Markers of tumor-initiating cells predict chemoresistance in breast cancer: C. Gong, et al.; PLoS One 5, e15630 (2010), Abstract; Full Text
Overexpression of Toll-like receptor 2/4 on monocytes modulates the activities of CD4(+)CD25(+) regulatory T cells in chronic hepatitis B virus infection: Y. Zhang, et al.; Virology 397, 34 (2010), Abstract;
Synthesis, properties and in vitro photodynamic activity of water-soluble azaphthalocyanines and azanaphthalocyanines: P. Zimcik, et al.; Photochem. Photobiol. 86, 168 (2010), Abstract;
COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells: H. Radilova, et al.; Mol. Cell Biochem. 330, 131 (2009), Abstract;
Interleukin-1beta increases gap junctional communication among synovial fibroblasts via the extracellular-signal-regulated kinase pathway: C. Niger, et al.; Biol. Cell 102, 37 (2009), Abstract; Full Text
Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect: Y. Kim, et al.; J. Biol. Chem. 280, 23273 (2005), Abstract; Full Text
Cyclin G2 dysregulation in human oral cancer: Y. Kim, et al.; Cancer Res. 64, 8980 (2004), Abstract; Full Text
Mitogen-activated protein kinases mediate peroxynitrite-induced cell death in human bronchial epithelial cells: E. Nabeyrat, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 284, L1112 (2003), Abstract; Full Text
Increased cytotoxicity of cadmium in fibroblasts lacking c-fos: M. Matsuoka, et al.; Biochem. Pharmacol. 59, 1573 (2000), Abstract;
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