Suitable for previously limiting low input samples, including precious biopsy samples
Validated for blood, amniotic fluid, postnatal, and oncology samples
Provides excellent dynamic analytical range down to 50 ng
Shorter workflow has samples ready for hybridization in < 4 hours
Superior DLR scores (0.09-0.12) exceeding industry standards
Ability to perform total genomic DNA analysis without amplification or complexity reduction
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
With optimized, proprietary reagents, Enzo's CYTAG® SuperCGH Labeling Kits for oligo arrays produce high quality data using as little as 50 ng of genomic DNA, without a need for pre-amplification for precious low input samples.
Analysis of patient samples using 50 ng DNA input on oligonucleotide microarray (Agilent 4 x 180K, CGH); A) Patient with Trisomy 9 showing duplication of Chromosome 9 (9p24.2-p11.2): DLRs = 0.149; B) Patient with Jacobsen Syndrome showing deletion of Chromosome 11 (11q24.1-q25): DLRs = 0.123; C) Amniotic sample 3: DLRs = 0.121; D) Amniotic sample 4: DLRs = 0.125
50 ng DNA samples were labeled with Enzo's CYTAG® SuperCGH Labeling Kit for Oligo Arrays or a leading competitor's kit. Enzo's proprietary labeling technology generates the highest specific activity of labeling for Cy3 and Cy5.
50 ng DNA samples were labeled with Enzo's CYTAG® SuperCGH Labeling Kit for Oligo Arrays or a leading competitor's kits.
Comparative analysis of 50 ng DNA samples labeled with Enzo's CYTAG® SuperCGH Labeling Kit or that of a leading competitor demonstrates significantly lower DLR scores.
CYTAG® SuperCGH labels DNA across a dynamic analytical range
50 ng DNA input from 4 different amniotic prenatal samples were labeled using CYTAG® SuperCGH Labeling Kit and applied to an 8 x 60K array, showing consistently low DLR scores.
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Product Details
Applications:
CGH
Application Notes:
Validated on Agilent SurePrint Microarrays.
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Handling:
Protect Super Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
Shipping:
Dry Ice and Ambient Temperature
Long Term Storage:
-20°C
Contents:
-0010 size: ENZ-GEN120-0010: Primers/Reaction Buffer, 1 x 400 µL; Super Cyanine 3-dUTP Nucleotide Mix, 1 x 100 µL; Super Cyanine 5-dUTP Nucleotide Mix, 1 x 100 µL; Klenow DNA Polymerase, 1 x 40 µL; Stop Buffer, 1 x 100 µL; Nuclease-free Water, 1 x 1 mL; PCR & Gel Clean-up columns, 20 tests -0100 size: ENZ-GEN120-0100: Primers/Reaction Buffer, 1 x 4 mL; Super Cyanine 3-dUTP Nucleotide Mix, 1 x 1 mL; Super Cyanine 5-dUTP Nucleotide Mix, 1 x 1 mL; Klenow DNA Polymerase, 1 x 0.4 mL; Stop Buffer, 1 x 1 mL; Nuclease-free Water, 1 x 10 mL; PCR & Gel Clean-up columns, 200 tests
PCR & Gel Clean-up Columns can be re-ordered using ENZ-GEN100
Restriction enzyme for molecular biology applications
Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides., Southern Blot, Cloning, Genotyping, CGH | Print as PDF
