Validated for use in aCGH microarrays and FISH assays
Decreases background noise to improve signal fidelity
Prevents nonspecific binding and cross-hybridization in samples
Human Cot DNA blocks repetitive sequences of DNA to suppress the level of cross-hybridization as well as non-specific binding to array surfaces. Utilizing Human Cot DNA in hybridizations increases overall sensitivity and improves experimental results.
Product Details
Alternative Name:
C0t DNA
Quantity:
500 μL
Concentration:
1 μg/μL
Formulation:
Liquid. In 10 mM TrisHCl, pH 7.5, containing 1 mM EDTA.
Applications:
ISH (in situ hybridization), Microarray, Southern Blot, FISH, CGH
Application Notes:
Human Cot DNA can be used to block repetitive DNA sequences in a variety of genomics applications.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-20°C
Scientific Background:
Repetitive sequences of DNA are found throughout the human genome. These repetitive DNA elements often present difficulties in acquiring clean results from hybridization processes due to nonspecific binding of probes. Cot DNA limits the background effects of nonspecific binding by suppressing cross-hybridization of these repetitive sequences.
Human Cot DNA is derived from the placental DNA and is prepared by shearing, denaturing, and reannealing under conditions that enrich repetitive elements.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis: J.K. Eykelenboom, et al.; J. Cell Biol. 218, 1531 (2019), Application(s): Generation of FISH probes, Abstract; Full Text
