| ENZ-GEN108-1000 | 1000 U | 106.00 USD |  |
||
| Do you need bulk/larger quantities? | |||||
| Source: | Produced in an E. coli strain that carries the cloned AluI gene from Arthrobacter luteus. |
| Concentration: | AluI 10,000 units/ml Includes 10X Cutting Buffer |
| Formulation: | Liquid. In 100mM KCl, containing 10mM Tris-HCl, pH 7.4, 1mM DTT, 0.1mM EDTA, 50% glycerol and 200µg/ml BSA. After reconstitution, 1X Cutting Buffer: 50mM potassium acetate, 20mM Tris-acetate, 10mM magnesium acetate, 100µg/ml BSA, pH 7.9, at 25°C. |
| Quality Control: | Exonuclease Activity (Radioactivity Release): A 50 µl reaction in 1X Cutting Buffer containing 1 µg of a mixture of single and double stranded [3H] E. coli DNA and a minimum of 30 units of AluI incubated for 4 hours at 37°C releases <0.1% of the total radioactivity. Ligation and Recutting (Terminal Integrity): After a 10-fold over digestion of λ DNA with AluI, ~75% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16°C. Of these ligated fragments, >95% can be recut with AluI. Non-Specific DNase Activity (16 Hour): A 50 µl reaction in 1X Cutting Buffer containing 1 µg of λ DNA and a minimum of 50 Units of AluI incubated for 16 hours at 37°C results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. |
| Activity: | One unit is defined as the amount of enzyme required to digest 1µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50µl. |
| Applications: | Southern Blot, Cloning, Genotyping, CGH |
| Application Notes: | For molecular cloning, restriction site mapping, genotyping, Southern blotting, SNP. |
| Reconstitution: | Reconstitute 10X Cutting Buffer with nuclease-free water. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Protocol: | Reaction conditions: 1X Cutting Buffer 37°C Heat inactivation: 80°C for 20 min |
| Regulatory Status: | RUO - Research Use Only |
CYTAG® CGH Labeling kit |
||||||
| Superior labeling efficiency and better dye incorporation results in less failed runs | ||||||
| CGH | Print as PDF | ||||||
| ENZ-42671-K010 | 2x10 Reactions | Inquire for pricing | ||||
| ENZ-42671-K100 | 2x100 Reactions | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
CGH labeling kit for BAC arrays |
||||||
| CGH | Print as PDF | ||||||
| ENZ-42670 | 20 Reactions | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
RsaI |
||||||
| Restriction enzyme for molecular biology applications | ||||||
| Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides., Southern Blot, Cloning, Genotyping, CGH | Print as PDF | ||||||
| ENZ-GEN109-1000 | 1000 U | 74.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
CYTAG® TotalCGH Labeling kit |
||||||
| Complete kit to digest and label DNA for CGH+SNP arrays | ||||||
| CGH | Print as PDF | ||||||
| ENZ-42674-0010 | 2x10 Reactions | Inquire for pricing | ||||
| ENZ-42674-0100 | 2x100 Reactions | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
