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Automated Liquid Handling for High Throughput DNA Labeling with CYTAG® SuperCGH Labeling Kit

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Jean-Michel Lapierre1, Valérie Malan1, Rosaria Esposito2, Morgan Mathieu2
1Hôpital Necker-Enfants Malades, Paris, France
2Enzo Life Sciences, Lausen, Switzerland

Featured Product: CYTAG® SuperCGH LABELING KIT


INTRODUCTION

Microarray-based comparative genomic hybridization (array CGH) is a fast genome-wide screening tool for the detection of a large number of chromosomal anomalies in the form of copy number variations (CNVs).

The cytogenetic department of the Hôpital Necker-Enfants Malades in Paris (France) carries out hundreds of prenatal, postnatal, and oncological CGH tests per year on a variety of specimens, including amniotic fluid, blood, bone marrow, fresh and formalin-fixed paraffin embedded (FFPE) tissues. Since the introduction of an automated liquid handling workstation (Freedom EVO 100, Tecan), this service handles almost 2,000 samples per year, keeping its usual high standards of reproducibility and security.

The objective of this study was to verify the compatibility of Enzo’s CYTAG® SuperCGH Labeling Kit with Tecan’s automated instrument.

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