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TNF-α (mouse), ELISA kit



ADI-900-047	96 wells	596.00 USD
Do you need bulk/larger quantities?

Stable color coded reagents and pre-coated plates reduce errors
Assay up to 38 samples in just 5 hours
Save time, money, and precious sample compared to Western blot analysis

The TNF-α (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 5 hours. Absorbance is read at 450 nm.

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Product Details

Alternative Name:	Tumor necrosis factor-α, TNFSF 2

Sensitivity:	3.9 pg/ml (range 15.63 - 2,000 pg/ml)

Assay Time:	5 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of mouse TNF-α in culture supernatants and serum. Cited sample type includes tissue (ref. 1) and plasma.

Wavelength:	450 nm

Species reactivity:	Mouse

Use/Stability:	Store all components at 4°C, except standard at -20°C.

Shipping:	Dry Ice and Blue Ice

Contents:	Microtiter plate, Conjugate, Antibody, Assay buffer 16, Wash buffer concentrate, Standard, TMB Substrate, Stop solution

Scientific Background:	Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.

Technical Info/Product Notes:	Cited samples: Cytokine ELISAs Cited Samples

UniProt ID:	P06804

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice: I.O. Shmarakov, et al.; Nat. Commun. 14, 1851 (2023), Abstract;

Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female Mice: C.R. García, et al.; Mol. Nutr. Food Res. 66, e2200082 (2022), Abstract;

Female reproductive life span is extended by targeted removal of fibrotic collagen from the mouse ovary: T. Umehara, et al.; Sci. Adv. 8, eabn4564 (2022), Abstract;

Tumor Necrosis Factor-α Mediates Lung Injury in the Early Phase of Endotoxemia: K.Y. Chen, et al.; Pharmaceuticals 15, 287 (2022), Abstract;

4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci. 37, 604 (2021), Application(s): IL-6 quantification in mouse blood, Abstract;

Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells: A. Karthikeyan, et al.; Plants 10, 2209 (2021), Abstract;

BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res. 169, 105611 (2021), Abstract;

Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H. U. Shih, et al.; J. Pers. Med. 11, 436 (2021), Abstract;

The lymphocyte adapter protein: A negative regulator of myocardial ischemia/reperfusion injury: J. Huang, et al.; J. Mol. Cell. Cardiol. 134, 107 (2019), Application(s): ELISA using mouse serum, Abstract;

Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway: Y. Lu, et al.; Hepatology 67, 1303 (2018), Application(s): ELISA using mouse serum, Abstract; Full Text

In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection: Y.H. Li, et al.; Int. J. Mol. Med. 41, 3620 (2018), Application(s): ELISA using mouse serum, Abstract;

Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model: G.U. Hong, et al.; Toxicol. Appl. Pharmacol. 358, 10 (2018), Application(s): ELISA using supernatants prepared from tissue homogenates, Abstract;

Smad proteins differentially regulate obesity-induced glucose and lipid abnormalities and inflammation via class-specific control of AMPK-related kinase MPK38/MELK activity: H.A. Seong, et al.; Cell Death Dis. 9, 471 (2018), Application(s): ELISA using mouse serum, Abstract; Full Text

Immunomodulation of cell-penetrating tat-metallothionein for successful outcome of xenotransplanted pancreatic islet: M.J. Kim, et al.; J. Drug Target. 25, 350 (2017), Application(s): Cell cutlure supernatant, Abstract;

The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity: B. Shan, et al.; Nat. Immunol. 18, 519 (2017), Abstract;

Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text

Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: Biochemical and histopathological studies: M. Abdel-Daim, et al. ; Biomed. Pharmacother. 77, 79 (2016), Application(s): Measurement of TNF-α in mouse serum, Abstract;

CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun. 478, 935 (2016), Application(s): Measurement of cytokine levels in serum and cell culture medium, Abstract;

Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata: C.G. Lee, et al.; Int. J. Biol. Macromol. 85, 505 (2016), Application(s): Measured concentration of TNF-α released from the cells treated with T. cuspidata, Abstract;

Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Application(s): IL-6 measured in mouse blood samples, Abstract; Full Text

The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother. 81, 111 (2016), Application(s): ELISA using mouse blood samples, Abstract;

Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol. 29, 344 (2015), Application(s): ELISA using culture supernatants, Abstract;

The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol. 31, 88 (2015), Application(s): ELISA using cell culture supernatants, Abstract;

Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury: Y. Zhang, et al.; Basic Res. Cardiol. 109, 434 (2014), Application(s): ELISA using mouse serum, Abstract;

Naltrexone attenuates endoplasmic reticulum stress induced hepatic injury in mice: A. Moslehi, et al.; Acta Physiol. Hung. 101, 341 (2014), Application(s): ELISA using mouse liver tissue, Abstract;

Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep. 40, 269 (2013), Application(s): EIA using culture supernatants from RAW264.7 macrophage cells, Abstract;

Early systemic inflammatory response in mice after a single oral gavage with live Escherichia coli is evidenced by increased TNF-alpha and nitric oxide production: A. Nemec, et al.; Res. Vet. Sci. 92, 401 (2012), Application(s): EIA using mouse plasma, Abstract;

Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Application(s): EIA using mouse serum and cell culture supernatant, Abstract; Full Text

Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab. 101, 66 (2010), Application(s): EIA using mouse serum, Abstract; Full Text

Acid sphingomyelinase involvement in tumor necrosis factor α-regulated vascular and steroid disruption during luteolysis in vivo: L.E. Henkes, et al. ; PNAS 105, 7670 (2008), Application(s): EIA using mouse tissue (ref. 1), Abstract;

Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes: O. Desy, et al. ; J. Immunol. 181, 2348 (2008), Application(s): EIA using mouse serum, Abstract;

Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol. 150, 332 (2007), Application(s): EIA using mouse tissue homogenate, Abstract; Full Text