- Stable color coded reagents and pre-coated plates reduce errors
- Assay up to 38 samples in just 5 hours
- Save time, money, and precious sample compared to Western blot analysis
The TNF-α (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 5 hours. Absorbance is read at 450 nm.
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Product Details
| Alternative Name: | Tumor necrosis factor-α, TNFSF 2 |
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| Sensitivity: | 3.9 pg/ml (range 15.63 - 2,000 pg/ml) |
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| Assay Time: | 5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of mouse TNF-α in culture supernatants and serum. Cited sample type includes tissue (ref. 1) and plasma. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 16, Wash buffer concentrate, Standard, TMB Substrate, Stop solution |
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| Scientific Background: | Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P06804 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Biflavonoid quercetin protects against cyclophosphamide–induced organ toxicities via modulation of inflammatory cytokines, brain neurotransmitters, and astrocyte immunoreactivity: A.Y. Onaolapo, et al.; Food Chem. Toxicol.
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Exploring the therapeutic efficacy of Chlorella pyrenoidosa peptides in ameliorating Alzheimer’s disease: S.M. Wang, et al.; Heliyon
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Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice: I.O. Shmarakov, et al.; Nat. Commun.
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Abstract;
Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female Mice: C.R. García, et al.; Mol. Nutr. Food Res.
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Effects of Suberoylanilide Hydroxamic Acid (SAHA) on the Inflammatory Response in Lipopolysaccharide-Induced N9 Microglial Cells: Z.B. Çelik, et al.; Cureus
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Abstract;
Female reproductive life span is extended by targeted removal of fibrotic collagen from the mouse ovary: T. Umehara, et al.; Sci. Adv.
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Abstract;
Tumor Necrosis Factor-α Mediates Lung Injury in the Early Phase of Endotoxemia: K.Y. Chen, et al.; Pharmaceuticals
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Abstract;
4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci.
37, 604 (2021),
Application(s): IL-6 quantification in mouse blood,
Abstract;
Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells: A. Karthikeyan, et al.; Plants
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BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res.
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Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H. U. Shih, et al.; J. Pers. Med.
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Abstract;
The lymphocyte adapter protein: A negative regulator of myocardial ischemia/reperfusion injury: J. Huang, et al.; J. Mol. Cell. Cardiol.
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Application(s): ELISA using mouse serum,
Abstract;
Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway: Y. Lu, et al.; Hepatology
67, 1303 (2018),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection: Y.H. Li, et al.; Int. J. Mol. Med.
41, 3620 (2018),
Application(s): ELISA using mouse serum,
Abstract;
Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model: G.U. Hong, et al.; Toxicol. Appl. Pharmacol.
358, 10 (2018),
Application(s): ELISA using supernatants prepared from tissue homogenates,
Abstract;
Smad proteins differentially regulate obesity-induced glucose and lipid abnormalities and inflammation via class-specific control of AMPK-related kinase MPK38/MELK activity: H.A. Seong, et al.; Cell Death Dis.
9, 471 (2018),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
Immunomodulation of cell-penetrating tat-metallothionein for successful outcome of xenotransplanted pancreatic islet: M.J. Kim, et al.; J. Drug Target.
25, 350 (2017),
Application(s): Cell cutlure supernatant,
Abstract;
The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity: B. Shan, et al.; Nat. Immunol.
18, 519 (2017),
Abstract;
Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol.
20, 415 (2016),
Application(s): ELISA using culture supernatants,
Abstract;
Full Text
Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: Biochemical and histopathological studies: M. Abdel-Daim, et al. ; Biomed. Pharmacother.
77, 79 (2016),
Application(s): Measurement of TNF-α in mouse serum,
Abstract;
CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun.
478, 935 (2016),
Application(s): Measurement of cytokine levels in serum and cell culture medium,
Abstract;
Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata: C.G. Lee, et al.; Int. J. Biol. Macromol.
85, 505 (2016),
Application(s): Measured concentration of TNF-α released from the cells treated with T. cuspidata,
Abstract;
Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci.
10, 21 (2016),
Application(s): IL-6 measured in mouse blood samples,
Abstract;
Full Text
The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother.
81, 111 (2016),
Application(s): ELISA using mouse blood samples,
Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol.
29, 344 (2015),
Application(s): ELISA using culture supernatants,
Abstract;
The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol.
31, 88 (2015),
Application(s): ELISA using cell culture supernatants,
Abstract;
Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury: Y. Zhang, et al.; Basic Res. Cardiol.
109, 434 (2014),
Application(s): ELISA using mouse serum,
Abstract;
Naltrexone attenuates endoplasmic reticulum stress induced hepatic injury in mice: A. Moslehi, et al.; Acta Physiol. Hung.
101, 341 (2014),
Application(s): ELISA using mouse liver tissue,
Abstract;
Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep.
40, 269 (2013),
Application(s): EIA using culture supernatants from RAW264.7 macrophage cells,
Abstract;
Early systemic inflammatory response in mice after a single oral gavage with live Escherichia coli is evidenced by increased TNF-alpha and nitric oxide production: A. Nemec, et al.; Res. Vet. Sci.
92, 401 (2012),
Application(s): EIA using mouse plasma,
Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum.
62, 732 (2010),
Application(s): EIA using mouse serum and cell culture supernatant,
Abstract;
Full Text
Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab.
101, 66 (2010),
Application(s): EIA using mouse serum,
Abstract;
Full Text
Acid sphingomyelinase involvement in tumor necrosis factor α-regulated vascular and steroid disruption during luteolysis in vivo: L.E. Henkes, et al. ; PNAS
105, 7670 (2008), Application(s): EIA using mouse tissue (ref. 1),
Abstract;
Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes: O. Desy, et al. ; J. Immunol.
181, 2348 (2008),
Application(s): EIA using mouse serum,
Abstract;
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol.
150, 332 (2007),
Application(s): EIA using mouse tissue homogenate,
Abstract;
Full Text
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