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TNF-α (mouse), ELISA kit

ADI-900-047 96 wells 596.00 USD
Do you need bulk/larger quantities?
  • Stable color coded reagents and pre-coated plates reduce errors
  • Assay up to 38 samples in just 5 hours
  • Save time, money, and precious sample compared to Western blot analysis
The TNF-α (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 5 hours. Absorbance is read at 450 nm.
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Product Details

Alternative Name:Tumor necrosis factor-α, TNFSF 2
Sensitivity:3.9 pg/ml (range 15.63 - 2,000 pg/ml)
Assay Time:5 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of mouse TNF-α in culture supernatants and serum. Cited sample type includes tissue (ref. 1) and plasma.
Wavelength:450 nm
Species reactivity:Mouse
Use/Stability:Store all components at 4°C, except standard at -20°C.
Shipping:Dry Ice and Blue Ice
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 16, Wash buffer concentrate, Standard, TMB Substrate, Stop solution
Scientific Background:Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
UniProt ID:P06804
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice: I.O. Shmarakov, et al.; Nat. Commun. 14, 1851 (2023), Abstract;
Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female Mice: C.R. García, et al.; Mol. Nutr. Food Res. 66, e2200082 (2022), Abstract;
Female reproductive life span is extended by targeted removal of fibrotic collagen from the mouse ovary: T. Umehara, et al.; Sci. Adv. 8, eabn4564 (2022), Abstract;
Tumor Necrosis Factor-α Mediates Lung Injury in the Early Phase of Endotoxemia: K.Y. Chen, et al.; Pharmaceuticals 15, 287 (2022), Abstract;
4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci. 37, 604 (2021), Application(s): IL-6 quantification in mouse blood, Abstract;
Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells: A. Karthikeyan, et al.; Plants 10, 2209 (2021), Abstract;
BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res. 169, 105611 (2021), Abstract;
Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H. U. Shih, et al.; J. Pers. Med. 11, 436 (2021), Abstract;
The lymphocyte adapter protein: A negative regulator of myocardial ischemia/reperfusion injury: J. Huang, et al.; J. Mol. Cell. Cardiol. 134, 107 (2019), Application(s): ELISA using mouse serum, Abstract;
Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway: Y. Lu, et al.; Hepatology 67, 1303 (2018), Application(s): ELISA using mouse serum, Abstract; Full Text
In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection: Y.H. Li, et al.; Int. J. Mol. Med. 41, 3620 (2018), Application(s): ELISA using mouse serum, Abstract;
Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model: G.U. Hong, et al.; Toxicol. Appl. Pharmacol. 358, 10 (2018), Application(s): ELISA using supernatants prepared from tissue homogenates, Abstract;
Smad proteins differentially regulate obesity-induced glucose and lipid abnormalities and inflammation via class-specific control of AMPK-related kinase MPK38/MELK activity: H.A. Seong, et al.; Cell Death Dis. 9, 471 (2018), Application(s): ELISA using mouse serum, Abstract; Full Text
Immunomodulation of cell-penetrating tat-metallothionein for successful outcome of xenotransplanted pancreatic islet: M.J. Kim, et al.; J. Drug Target. 25, 350 (2017), Application(s): Cell cutlure supernatant, Abstract;
The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity: B. Shan, et al.; Nat. Immunol. 18, 519 (2017), Abstract;
Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text
Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: Biochemical and histopathological studies: M. Abdel-Daim, et al. ; Biomed. Pharmacother. 77, 79 (2016), Application(s): Measurement of TNF-α in mouse serum, Abstract;
CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun. 478, 935 (2016), Application(s): Measurement of cytokine levels in serum and cell culture medium, Abstract;
Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata: C.G. Lee, et al.; Int. J. Biol. Macromol. 85, 505 (2016), Application(s): Measured concentration of TNF-α released from the cells treated with T. cuspidata, Abstract;
Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Application(s): IL-6 measured in mouse blood samples, Abstract; Full Text
The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother. 81, 111 (2016), Application(s): ELISA using mouse blood samples, Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol. 29, 344 (2015), Application(s): ELISA using culture supernatants, Abstract;
The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol. 31, 88 (2015), Application(s): ELISA using cell culture supernatants, Abstract;
Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury: Y. Zhang, et al.; Basic Res. Cardiol. 109, 434 (2014), Application(s): ELISA using mouse serum, Abstract;
Naltrexone attenuates endoplasmic reticulum stress induced hepatic injury in mice: A. Moslehi, et al.; Acta Physiol. Hung. 101, 341 (2014), Application(s): ELISA using mouse liver tissue, Abstract;
Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep. 40, 269 (2013), Application(s): EIA using culture supernatants from RAW264.7 macrophage cells, Abstract;
Early systemic inflammatory response in mice after a single oral gavage with live Escherichia coli is evidenced by increased TNF-alpha and nitric oxide production: A. Nemec, et al.; Res. Vet. Sci. 92, 401 (2012), Application(s): EIA using mouse plasma, Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Application(s): EIA using mouse serum and cell culture supernatant, Abstract; Full Text
Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab. 101, 66 (2010), Application(s): EIA using mouse serum, Abstract; Full Text
Acid sphingomyelinase involvement in tumor necrosis factor α-regulated vascular and steroid disruption during luteolysis in vivo: L.E. Henkes, et al. ; PNAS 105, 7670 (2008), Application(s): EIA using mouse tissue (ref. 1), Abstract;
Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes: O. Desy, et al. ; J. Immunol. 181, 2348 (2008), Application(s): EIA using mouse serum, Abstract;
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol. 150, 332 (2007), Application(s): EIA using mouse tissue homogenate, Abstract; Full Text

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