Product Details
| Alternative Name: | COL1 |
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| MW: | 235kDa, 215kDa, 130kDa, 115kDa |
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| Source: | Isolated from rat tail tendons. |
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| Concentration: | 100µg/ml |
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| Formulation: | Sterile liquid. In 60% ethanol. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <100 EU/mg purified protein (LAL test) |
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| Appearance: | Clear liquid. |
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| Activity: | Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips. |
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| Application Notes: | Can be used for thin layer coating of surfaces (culture plates, slides, coverslips) |
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| Shipping: | Blue Ice Not Frozen - Dangerous Good |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Handling: | Do not expose to flames. Keep sterile. Do not freeze. |
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| Scientific Background: | Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment. |
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| Protocol: | Thin coating procedure - Ready to use formulation.
- Coat at 100µl/cm2.
- Place the collagen coated surface uncovered in a tissue culture hood overnight or until completely dry.
- Store at +4°C until use.
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1. Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.
Figure 2. Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Collagen I, Rat Tail.
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Product Literature References
Axon guidance receptor ROBO3 modulates subtype identity and prognosis via AXL-associated inflammatory network in pancreatic cancer: N. Krebs, et al.; JCI Insight
7, e154475 (2022),
Abstract;
A rapid and efficient method for dissociated cultures of mouse myenteric neurons: G. Wahba, et al.; J. Neurosci. Methods
261, 110 (2016),
Abstract;
General Literature References
Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tube: M.B. Bornstein, et al.; Lab. Invest. 134, (1958),
