| ENZ-60001-C001 | 1 µg | 199.00 USD |  |
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| MW: | ~40kDa (SDS-PAGE) |
| Source: | Cell culture |
| UniProt ID: | P27467 |
| Formulation: | Lyophilized solid. |
| Purity: | >75% by SDS-PAGE and >75% by HPLC analyses |
| Endotoxin Content: | Less than 0.2 ng per µg (2EU/µg) |
| Biological Activity: | Wnt-3a can induce β-catenin mediated response in a TCF/LEF reporter gene luciferase assay. |
| Reconstitution: | Prepare 5 μg/mL stock solution of Wnt3a by adding 200 μL deionized water into the vial of lyophilized Wnt3a. |
| Shipping: | Dry Ice |
| Short Term Storage: | -80°C |
| Long Term Storage: | -80°C |
| Use/Stability: | Stable for at least 12 months after receipt when stored, as supplied, at -80°C. Following reconstitution, stable for three months when stored at or below -20°C. |
| Scientific Background: | Wnt expression and signal transduction have been identified as critical components in regulating embryogenesis, cell proliferation, and bone, glucose and lipid metabolism. Wnt-3a signalling also plays a role in regulating the differentation of stem cells. Wnt-3a is a member of the highly conserved, structurally related cysteine-rich Wnt family glycoproteins important for normal developmental processes and oncogenesis. Wnt-3a proteins are secreted and bind to receptors of the Frizzled and LRP families on the cell surface, which leads to the stabilization of β-catenin and increase of β-catenin translocation into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. The predicted mouse Wnt-3a protein of 352 amino acid residues and contains an N-terminal signal peptide for secretion. It shares 96% amino acid identity to human Wnt-3a protein. Due to glycosylation, it migrates at an apparent molecular weight of ~40 kDa by SDS-PAGE analysis under reducing conditions. |
| Technical Info/Product Notes: | Application Note: New LEADING LIGHT Wnt Reporter Assay with Highest Sensitivity in Response to Wnt Effectors |
| Regulatory Status: | RUO - Research Use Only |
Dishevelled-1 polyclonal antibody |
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| Purified from rabbit serum., WB | Print as PDF | ||||||
| BML-DA4170-0100 | 100 µl | 445.00 USD | |
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Dishevelled-2 polyclonal antibody |
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| Purified from rabbit serum., IHC, WB | Print as PDF | ||||||
| BML-DA4270-0025 | 25 µl | 137.00 USD | |
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| BML-DA4270-0100 | 100 µl | 444.00 USD | |
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Dkk-1 (mouse), (recombinant) (His-tag) |
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| Produced in E. coli. Mouse Dkk-1 is fused at the N-terminus to a His-tag., ≥95% (SDS-PAGE; Western blot), WB | Print as PDF | ||||||
| ADI-KPR-CP100-D | 50 µg | 629.00 USD | |
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| ADI-KPR-CP100-F | 200 µg | 1,170.00 USD | |
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GSK-3β polyclonal antibody |
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| Purified from rabbit serum., ICC, IHC (PS), WB | Print as PDF | ||||||
| ADI-KAP-ST002-C | 25 µg | 170.00 USD | |
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| ADI-KAP-ST002-E | 100 µg | 367.00 USD | |
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GSK-3β (human), (recombinant) (GST-tag) |
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| Produced in insect cells. Full length human glycogen synthase kinase-3ß is fused at the N-terminus to a GST-tag. Produced in a baculovirus expression system. | Print as PDF | ||||||
| BML-SE355-0005 | 5 µg | 338.00 USD | |
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GSK3 substrate |
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| Print as PDF | ||||||
| BML-P151-0001 | 1 mg | 174.00 USD | |
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