Wnt-3a can induce β-catenin mediated response in a TCF/LEF reporter gene luciferase assay.
Reconstitution:
Prepare 5 μg/mL stock solution of Wnt3a by adding 200 μL deionized water into the vial of lyophilized Wnt3a.
Shipping:
Shipped on Dry Ice
Short Term Storage:
-80°C
Long Term Storage:
-80°C
Use/Stability:
Stable for at least 12 months after receipt when stored, as supplied, at -80°C. Following reconstitution, stable for three months when stored at or below -20°C.
Scientific Background:
Wnt expression and signal transduction have been identified as critical components in regulating embryogenesis, cell proliferation, and bone, glucose and lipid metabolism. Wnt-3a signalling also plays a role in regulating the differentation of stem cells. Wnt-3a is a member of the highly conserved, structurally related cysteine-rich Wnt family glycoproteins important for normal developmental processes and oncogenesis. Wnt-3a proteins are secreted and bind to receptors of the Frizzled and LRP families on the cell surface, which leads to the stabilization of β-catenin and increase of β-catenin translocation into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. The predicted mouse Wnt-3a protein of 352 amino acid residues and contains an N-terminal signal peptide for secretion. It shares 96% amino acid identity to human Wnt-3a protein. Due to glycosylation, it migrates at an apparent molecular weight of ~40 kDa by SDS-PAGE analysis under reducing conditions.
Produced in insect cells. Full length human glycogen synthase kinase-3ß is fused at the N-terminus to a GST-tag. Produced in a baculovirus expression system. | Print as PDF
