MW:	~40kDa (SDS-PAGE)

Source:	Cell culture

UniProt ID:	P27467

Formulation:	Lyophilized solid.

Purity:	>75% by SDS-PAGE and >75% by HPLC analyses

Endotoxin Content:	Less than 0.2 ng per µg (2EU/µg)

Biological Activity:	Wnt-3a can induce β-catenin mediated response in a TCF/LEF reporter gene luciferase assay.

Reconstitution:	Prepare 5 μg/mL stock solution of Wnt3a by adding 200 μL deionized water into the vial of lyophilized Wnt3a.

Shipping:	Shipped on Dry Ice

Short Term Storage:	-80°C

Long Term Storage:	-80°C

Use/Stability:	Stable for at least 12 months after receipt when stored, as supplied, at -80°C. Following reconstitution, stable for three months when stored at or below -20°C.

Scientific Background:	Wnt expression and signal transduction have been identified as critical components in regulating embryogenesis, cell proliferation, and bone, glucose and lipid metabolism. Wnt-3a signalling also plays a role in regulating the differentation of stem cells. Wnt-3a is a member of the highly conserved, structurally related cysteine-rich Wnt family glycoproteins important for normal developmental processes and oncogenesis. Wnt-3a proteins are secreted and bind to receptors of the Frizzled and LRP families on the cell surface, which leads to the stabilization of β-catenin and increase of β-catenin translocation into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. The predicted mouse Wnt-3a protein of 352 amino acid residues and contains an N-terminal signal peptide for secretion. It shares 96% amino acid identity to human Wnt-3a protein. Due to glycosylation, it migrates at an apparent molecular weight of ~40 kDa by SDS-PAGE analysis under reducing conditions.

Technical Info/Product Notes:	Application Note: New LEADING LIGHT Wnt Reporter Assay with Highest Sensitivity in Response to Wnt Effectors

Regulatory Status:	RUO - Research Use Only

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