Replaces Prod. #: ALX-430-159
Cyclopamine is a naturally occuring steroidal alkaloid that causes cyclopia by blocking sonic hedgehog signaling. It specifically inhibits the hedgehog (Hh) pathway by interaction with the Hh signaling protein smoothened. Cyclopamine blocks the oncogenic effects of mutations of Ptch in fibroblasts and inhibits the growth of cells lacking Ptch function. It arrested cell cycle at G0/G1 and induced apoptosis in several small-cell lung cancer cell lines at 5 µM. Cyclopamine also induces expression of the transcription factor pdx-1, which stimulates insulin secretion in differentiation protocols for mouse embryonic stem cells
Product Details
Formula: | C27H41NO2 |
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MW: | 411.6 |
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CAS: | 4449-51-8 |
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Purity: | ≥98% (TLC) |
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Appearance: | white to off-white powder |
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Solubility: | Soluble in 100% ethanol (20mg/ml), DMSO (4mg/ml), dimethyl formamide (10mg/ml), methanol (7mg/ml). Insoluble in water. |
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Shipping: | Ambient |
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Long Term Storage: | -20°C |
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Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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Handling: | Protect from light. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Abstract;
Full Text
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Abstract;
Internalization and induction of antioxidant messages by microvesicles contribute to the antiapoptotic effects on human endothelial cells: R. Soleti, et al.; Free Radic. Biol. Med.
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Abstract;
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Abstract;
Full Text
Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment: D. Qualtrough, et al.; Int. J. Cancer.
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Abstract;
In vitro directed differentiation of mouse embryonic stem cells into insulin-producing cells: T. Leon-Quinto, et al.; Diabetologia
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Abstract;
Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity: S. Tabs & O. Avci; Eur. J. Dermatol.
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Abstract;
Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway: X. Wu, et al.; Chem. Biol.
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Abstract;
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Abstract;
Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis: S.P. Thayer, et al.; Nature
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Abstract;
Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancer: D.N. Watkins, et al.; Nature
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Abstract;
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Abstract;
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Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport: J.P. Incardona, et al.; Dev. Biol.
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Abstract;
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Teratogen-mediated inhibition of target tissue response to Shh signaling: M.K. Cooper, et al.; Science
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