For everyday PCR applications with increased sensitivity
Advanced hot-start Taq DNA polymerase
Convenient 2x Taq DNA polymerase mix
AMPIGENE® Hot Start Taq Mix uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme uses advanced hot-start technology for superior sensitivity. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA. Due to enhanced efficiency and specificity the enzyme is perfectly suited to difficult PCR. AMPIGENE® Hot Start Taq Mix is a robust enzyme for all your everyday PCR applications. AMPIGENE® Hot Start Taq Mix can perform consistently well on a broad range of templates (including both GC and AT rich). AMPIGENE® Hot Start Taq Mix has an error rate of approximately 1 error per 2.0 x 105 nucleotides incorporated. PCR products generated with AMPIGENE® Hot Start Taq Mix are A-tailed and may be cloned into TA cloning vectors.
PCR amplification of human genomic DNA using AMPIGENE® Hot Start Taq Mix. PCR amplification was performed using two different primer sets. Amplification products were analyzed on 1.2% agarose gel. Human genomic DNA (25 ng) was used as template. Annealing temperature was 60°C. Left lane, 1 kb ladder.
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Product Details
Applications:
PCR
Application Notes:
For standard PCR applications that require higher sensitivity
Shipping:
Blue Ice
Long Term Storage:
-20°C
Contents:
2x AMPIGENE® Hot Start Taq Mix (AMPIGENE® Hot Start Taq DNA Polymerase, 3.5 mM MgCl2, 0.4 mM dNTPs, enhancers and stabilizers)
