| Alternative Name: | GDF-5, CDMP-1, BMP-14, Bone morphogenetic protein 14, Cartilage-derived morphogenetic protein 1, LAP-4, LPS-associated protein 4, Radotermin |
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| MW: | ~28.8kDa |
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| Source: | Produced in E. coli. Non-glycosylated homodimer, containing two 117 amino acids chains. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys. |
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| UniProt ID: | P43026 |
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| Formulation: | Lyophilized. Sterile filtered. |
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| Purity: | ≥98% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells which is typically observed at a concentration of 0.1-1µg/ml. |
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| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Growth and differentiation factor 5 (GDF-5) is a growth factor that regulates cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is part of the TGF family of proteins and is closely related to the BMP family of proteins. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human GDF-5 is predicted to be a homodimer with a total MW of 26.8 kDa.
Figure 1: Human GDF-5 Bioactivity Data. Serial dilutions of human GDF-5, starting at 5µg/ml, were added to ATDC-5 cells growing in the presence of 4µg/ml heparin. Alkaline phosphate activity was measure after 67 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this specific lot Human GDF-5 was 0.01µg/ml. This value is comparable to the typical expected range of <1ng/ml.
