| Alternative Name: | FS, Activin-binding protein, FSH-suppressing protein |
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| MW: | ~31.7kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 289 amino acids. |
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| UniProt ID: | P19883 |
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| Formulation: | Lyophilized from 10mM sodium phosphate and 50mM sodium chloride, pH 7.5, sterile filtered. |
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| Purity: | ≥95% (HPLC, Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <0.1ng/ml. |
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| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-β family members (activin, BMPs, myostatin, GDF-11 and TGF-β1). Mechanistically, follistatin works as an antagonist by complexing with TGF-β family members to prevent them from interacting with their signaling receptors. |
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| Regulatory Status: | RUO - Research Use Only |
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