Alternative Name: | SCF, c-Kit ligand, KL, Steel factor, MGF |
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MW: | ~18.3kDa |
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Source: | Produced in E. coli. Non-glycosylated protein, containing 164 amino acids. |
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UniProt ID: | P20826 |
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Formulation: | Lyophilized from a sterile aqueous solution containing 20mM sodium phospate, pH 6.5. |
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Purity: | ≥95% (reducing and non-reducing SDS-PAGE) |
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Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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Appearance: | White lyophilized (freeze-dried) powder. |
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Biological Activity: | The activity is determined by the dose-dependent proliferation of human TF-1 cells which is typically observed at concentrations <10ng/ml. |
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Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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Shipping: | Ambient Temperature |
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Long Term Storage: | -20°C |
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Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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Scientific Background: | Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells. |
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Regulatory Status: | RUO - Research Use Only |
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